Effect of Salivary Stimulation on Erosion of Human and Bovine Enamel Subjected or Not to Subsequent Abrasion: An in situ/ex vivo Study

2006 ◽  
Vol 40 (3) ◽  
pp. 218-223 ◽  
Author(s):  
D. Rios ◽  
H.M. Honório ◽  
A.C. Magalhães ◽  
A.C.B. Delbem ◽  
M.A.A.M. Machado ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Bovine Enamel ◽  
Ex Vivo Study

scholarly journals Light cola drink is less erosive than the regular one: An in situ/ex vivo study

2009 ◽  
Vol 37 (2) ◽  
pp. 163-166 ◽  
Author(s):  
Daniela Rios ◽  
Heitor Marques Honório ◽  
Ana Carolina Magalhães ◽  
Annette Wiegand ◽  
Maria Aparecida de Andrade Moreira Machado ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Ex Vivo Study

scholarly journals Assessment of intraductal carcinoma in situ (DCIS) using grating-based X-ray phase-contrast CT at conventional X-ray sources: An experimental ex-vivo study

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210291 ◽  
Author(s):  
Karin Hellerhoff ◽  
Lorenz Birnbacher ◽  
Anikó Sztrókay-Gaul ◽  
Susanne Grandl ◽  
Sigrid Auweter ◽  
...  
Keyword(s):  
Carcinoma In Situ ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Intraductal Carcinoma ◽  
X Ray ◽  
Contrast Ct ◽  
Ex Vivo Study

Is the dentifrice containing calcium silicate, sodium phosphate, and fluoride able to protect enamel against chemical mechanical wear? An in situ/ex vivo study

2019 ◽  
Vol 23 (10) ◽  
pp. 3713-3720 ◽  
Author(s):  
Franciny Querobim Ionta ◽  
Natália Mello dos Santos ◽  
Isabela Maníglia Mesquita ◽  
Evandro José Dionísio ◽  
Thiago Cruvinel ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Sodium Phosphate ◽  
Ex Vivo ◽  
Mechanical Wear ◽  
Ex Vivo Study

scholarly journals Effect of 4% titanium tetrafluoride solution on the erosion of permanent and deciduous human enamel: an in situ/ex vivo study

2009 ◽  
Vol 17 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Ana Carolina Magalhães ◽  
Daniela Rios ◽  
Heitor Marques Honório ◽  
Alberto Carlos Botazzo Delbem ◽  
Marília Afonso Rabelo Buzalaf
Keyword(s):  
Ex Vivo ◽  
Titanium Tetrafluoride ◽  
Human Enamel ◽  
Ex Vivo Study

scholarly journals Effect of Tannic Acid on the Protective Properties of the in situ Formed Pellicle

2016 ◽  
Vol 51 (1) ◽  
pp. 34-45 ◽  
Author(s):  
Susann Hertel ◽  
Sandra Pötschke ◽  
Sabine Basche ◽  
Judith Delius ◽  
Wiebke Hoth-Hannig ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Ex Vivo ◽  
Oral Exposure ◽  
Dependent Manner ◽  
Pellicle Formation ◽  
Protective Properties ◽  
Transmission Electron ◽  
Bovine Enamel ◽  
The Impact

Objectives: In the present in situ/ex vivo study the impact of tannic acid on the erosion-protective properties of the enamel pellicle was tested. Additionally, the antiadherent and antibacterial effects of tannic acid were evaluated. Methods: The pellicle was formed in situ on bovine enamel samples fixed on individual splints worn by 6 subjects. Following 1 min of pellicle formation the volunteers rinsed for 10 min with tannic acid. After further oral exposure for 19 min, 109 min, and 8 h overnight, respectively, slabs were incubated in HCl ex vivo (pH 2.0, 2.3, 3.0) over 120 s. Subsequently, kinetics of calcium and phosphate release were measured photometrically. Samples after a 1-min fluoride mouth rinse as well as enamel samples with and without a 30-min in situ pellicle served as controls. Antiadherent effects were evaluated after a 1-min rinse with tannic acid and oral exposure of the slabs overnight. DAPI (4′,6-diamidino-2-phenylindole) combined with concanavalin A staining and live/dead staining was used for fluorescence microscopic visualization and quantification of adherent bacteria and glucans. Modification of the pellicle's ultrastructure by tannic acid was evaluated by transmission electron microscopy (TEM). Results: Tannic acid significantly improved the erosion-protective properties of the pellicle in a pH-dependent manner. Bacterial adherence and glucan formation on enamel were significantly reduced after rinses with tannic acid as investigated by fluorescence microscopy. TEM imaging indicated that rinsing with tannic acid yielded a sustainable modification of the pellicle; it was distinctly more electron dense. Conclusion: Tannic acid offers an effective and sustainable approach for the prevention of caries and erosion.

scholarly journals In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 67
Author(s):  
Richard Krombholz ◽  
Yali Liu ◽  
Dominique Jasmin Lunter
Keyword(s):  
Raman Spectroscopy ◽  
Ex Vivo ◽  
Skin Penetration ◽  
Confocal Raman Spectroscopy ◽  
Line Measurement ◽  
Lauryl Ether ◽  
Confocal Raman ◽  
Ex Vivo Study

Ex-vivo and in-vivo skin analysis has been extensively evaluated by confocal Raman spectroscopy (CRS). The off-line measurement with a CRS-suited skin-mounted device after Franz-cell incubations is the most popular choice. However, real-time monitoring of in-line measurement has clear advantages for obtaining dynamic and more timely results. In our study, a custom-built setup suitable for in-line measurements was implemented, which ensures constant skin incubation and in-situ skin detections. We aim to compare the differences between using in-line and off-line devices for monitoring skin drug penetrations. A well-assessed formulation gel with procaine-HCl as the active ingredient was used as reference. The PEG-23 lauryl ether was added to the formulation as a penetration enhancer to evaluate the enhancement effects of procaine on skin. After incubation times of 14, 20, and 24 h, skin penetration profiles were assessed. Comparable results between off-line and in-line measurements were obtained. Remarkable improvements in penetrated procaine amount and depth were observed. Based on the significant differences of their enhanced penetration amounts, fairly similar estimations were achieved from both methods. A slight difference of 14 h incubation between these two setups can still be found, which may be due to the different detection conditions and affected skin properties. Overall, in-line measurements could provide a more time- and labor-saving alternative for off-line measurements in ex-vivo study.

scholarly journals Effect of Toothpastes with Different Abrasives on Eroded Human Enamel: An in situ/ex vivo Study

2013 ◽  
Vol 7 (1) ◽  
pp. 132-139 ◽  
Author(s):  
Meire Coelho Ferreira ◽  
Maria Letícia Ramos-Jorge ◽  
Alberto Carlos Botazzo Delbem ◽  
Ricardo de Sousa Vieirac
Keyword(s):  
Ex Vivo ◽  
Soft Drink ◽  
Dental Enamel ◽  
Additional Treatment ◽  
Control Group ◽  
Wear Depth ◽  
Human Enamel ◽  
Waiting Periods ◽  
Ex Vivo Study

The aim of the present study was to investigate the abrasive effect of CaCO3 and SiO2-based fluoride-free experimental toothpastes on eroded human permanent dental enamel and evaluate the effectiveness of waiting periods between acid exposure and tooth brushing. Twelve volunteers wore palatal appliances containing human enamel blocks for two periods of five days each. The appliances were immersed in a soft drink for five minutes four times a day (9:00 am, 11:00 am, 2:00 pm and 4:00 pm). On two occasions, two blocks were not submitted to additional treatment; two blocks were brushed (30 s) either with a CaCO3 or SiO2 toothpaste immediately after erosion and two blocks were brushed 1 h after erosion. Thus, the sample was divided into six groups: erosion alone (CaCO3 and SiO2 control); brushing with fluoride-free toothpaste (CaCO3 immediate and 1 h after erosion; SiO2 immediate and 1 h after erosion). Significant differences in wear depth were found between the enamel blocks in the CaCO3 immediate and 1 h after erosion groups and the blocks in the CaCO3 control group (p=0.001; p=0.022). No significant differences were found regarding the change in roughness and wear depth between blocks submitted to immediate abrasion and 1 h after erosion (CaCO3 and SiO2). The data revealed that surface roughness and wear depth is increased when erosion is combined with dental abrasion, regardless of the abrasive used. Waiting for 1 h to brush the eroded blocks offered no protective effect.

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