Molecular Cloning of cDNA, Recombinant Protein Expression and Characterization of a Buckwheat 16-kDa Major Allergen

2006 ◽  
Vol 140 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Satoru Koyano ◽  
Kayoko Takagi ◽  
Reiko Teshima ◽  
Jun-ichi Sawada
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Molecular Cloning ◽  
Recombinant Protein Expression ◽  
Major Allergen

Molecular cloning, recombinant protein expression, tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen

2014 ◽  
Vol 94 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Peng Zhang ◽  
Yong Wang ◽  
Mingfeng Jiang ◽  
Lianlian Zhu ◽  
Jianbo Li ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Amino Acid ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Tissue Distribution ◽  
Molecular Mass ◽  
Molecular Cloning ◽  
Recombinant Protein Expression ◽  
Black Goat ◽  
Goat Rumen

Zhang, P., Wang, Y., Jiang, M., Zhu, L., Li, J., Luo, M., Ren, H. and Liu, L. 2014. Molecular cloning, recombinant protein expression, tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen. Can. J. Anim. Sci. 94: 27–34. Three major distinct types of lysozymes have been identified in the animal kingdom and most lysozymes cloned from ruminants belong to the chicken-type (c-type). In this study, a new c-type lysozyme gene, named LZRLyz, was cloned and sequenced from the Lezhi black goat rumen. The LZRLyz cDNA has a 444 bp open reading frame (ORF) encoding a 147 amino acid polypeptide. The encoded polypeptide is predicted to have an 18 amino acid signal peptide, and a 129 amino acid mature protein with an isoelectric point (pI) of 6.08. The LZRLyz amino acid sequence shares 70.27% identity with the Capra hircus blood lysozyme and is grouped with other ruminants c-type lysozymes using the phylogenetic tree estimated by Neighbor-Jointing method. The recombinant expressed LZRLyz protein (pET-rLZR) shows a molecular mass of ∼33 kDa, which is consistent with the predicted fusion protein molecular mass and shows antimicrobial activity. Quantitative real-time RT-PCR analyses revealed that LZRLyz transcripts are expressed in all tested tissues with the predominant expression being observed in rumen and the weakest one in spleen. Results of this study suggest that the LZRLyz gene represents a new c-type lysozyme gene that likely functions in Lezhi black goat host immunity and digestive systems.

scholarly journals Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum

2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Erdenedolgor Erdene‐Ochir ◽  
Bok‐Kyu Shin ◽  
Md Nazmul Huda ◽  
Eun Ha Lee ◽  
Dae‐Geun Song ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Phaeodactylum Tricornutum ◽  
Recombinant Protein Expression
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