Notch Signaling Is Required to Maintain All Neural Stem Cell Populations – Irrespective of Spatial or Temporal Niche

2006 ◽  
Vol 28 (1-2) ◽  
pp. 34-48 ◽  
Author(s):  
Tania O. Alexson ◽  
Seiji Hitoshi ◽  
Brenda L. Coles ◽  
Alan Bernstein ◽  
Derek van der Kooy
Keyword(s):  
Stem Cell ◽  
Notch Signaling ◽  
Neural Stem Cell ◽  
Cell Populations ◽  
Temporal Niche ◽  
Stem Cell Populations

scholarly journals Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

2013 ◽  
Vol 22 (20) ◽  
pp. 2777-2793 ◽  
Author(s):  
Barbara Orsolits ◽  
Adrienn Borsy ◽  
Emília Madarász ◽  
Zsófia Mészáros ◽  
Tímea Kőhidi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations

scholarly journals Designing signaling environments to steer transcriptional diversity in neural progenitor cell populations

2019 ◽  
Author(s):  
Jong H. Park ◽  
Tiffany Tsou ◽  
Paul Rivaud ◽  
Matt Thomson ◽  
Sisi Chen
Keyword(s):  
Cell Culture ◽  
Population Structure ◽  
Stem Cell ◽  
Single Cell ◽  
Neural Stem Cell ◽  
Cell Populations ◽  
List Type ◽  
Developmental Potential ◽  
Stem Cell Populations

AbstractStem cell populations within developing embryos are diverse, composed of many different sub-populations of cells with varying developmental potential. The structure of stem cell populations in cell culture remains poorly understood and presents a barrier to differentiating stem cells for therapeutic applications. In this paper we develop a framework for controlling the architecture of stem cell populations in cell culture using high-throughput single cell mRNA-seq and computational analysis. We find that the transcriptional diversity of neural stem cell populations collapses in cell culture. Cell populations are depleted of committed neuron progenitor cells and become dominated by a single pre-astrocytic cell population. By analyzing the response of neural stem cell populations to forty distinct signaling conditions, we demonstrate that signaling environments can restructure cell populations by modulating the relative abundance of pre-astrocyte and pre-neuron subpopulations according to a simple linear code. One specific combination of BMP4, EGF, and FGF2 ligands switches the default population balance such that 70% of cells correspond to the committed neurons. Our work demonstrates that single-cell RNA-seq can be applied to modulate the diversity of in vitro stem cell populations providing a new strategy for population-level stem cell control.HighlightsNatural progenitor diversity in the brain collapses during in vitro culture to a single progenitor typeLoss of progenitor diversity alters fate potential of cells during differentiationLarge scale single-cell signaling screen identifies signals that reshape population structure towards neuronal cell typesSignals regulate population structure according to a simple log-linear model

scholarly journals Distinct Neural Stem Cell Populations Give Rise to Disparate Brain Tumors in Response to N-MYC

Cancer Cell ◽  
2012 ◽  
Vol 21 (5) ◽  
pp. 601-613 ◽  
Author(s):  
Fredrik J. Swartling ◽  
Vasil Savov ◽  
Anders I. Persson ◽  
Justin Chen ◽  
Christopher S. Hackett ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Tumors ◽  
Neural Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations

scholarly journals Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag

2021 ◽  
Vol 2 (3) ◽  
pp. 100751
Author(s):  
Yuefeng Li ◽  
Kiran Nakka ◽  
Thomas Olender ◽  
Philippe Gingras-Gelinas ◽  
Matthew Man-Kin Wong ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations
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