Frequency of Chromosome 17 Aneuploidy in Primary and Recurrent Pterygium by Interphase-Fluorescence in situ Hybridization

2006 ◽  
Vol 38 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Umit Kamis ◽  
Hurkan Kerimoglu ◽  
Ahmet Ozkagnici ◽  
Hasan Acar
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome 17 ◽  
Recurrent Pterygium

Multiplex Fluorescence In Situ Hybridization (M-FISH) in the Detection of Complex Karyotypic Abnormalities of Acute Myeloid Leukemia and Myelodysplastic Syndromes.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4504-4504
Author(s):  
Jianyong Li ◽  
Jinlan Pan ◽  
Bing Xiao ◽  
Li Ma ◽  
Hairong Qiu ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Myelodysplastic Syndromes ◽  
Myeloid Leukemia ◽  
Chromosome Analysis ◽  
Chromosome 17 ◽  
Structural Aberrations ◽  
Acute Myeloid

Abstract The complex chromosome abnormalities (CCAs) were one of the most important poor prognostic risk factors in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Chromosome analysis using classical cytogenetic banding techniques fails to completely resolve complex karyotypes and cryptic translocations. The technique of multiplex fluorescence in situ hybridization (M-FISH) allow for the simultaneous visualization of all chromosomes of a metaphase in a single hybridization step and thereby enable to comprehensively analyze complex karyotypes and the identification of new and cryptic translocations. To investigate the value of M-FISH in the detection of complex karyotypic abnormalities of AML and MDS. M-FISH was used in combination with interphase-FISH to study 24 cases of AML and MDS with CCAs showed by R-banding of conventional cytogenetics (CC). In 14 cases of AML with CCAs, 4 gains of whole chromosome and 4 losses of whole chromosome were confirmed by M-FISH, while 12 losses of whole chromosome were revised as derivative chromosomes resulted from various structural aberrations. 26 derivative chromosomes and 19 marker chromosomes were characterized precisely by M-FISH. Most of them were unbalanced translocations, including 2 complex t(8;21), which have not been previously described:t(8;21), der(8) t(8;21) (8pter→8q22::21q22→21qter), der(21) t(8;21;8) (8qter→ 8q22::21p13→ 21q22::8q22→ 8qter) and t(21;8;18;1), der(8) t(8;21) (8pter→ 8q22::21q22→ 21qter), der(21) t(21;8;18;1) (21p13→ 21q22::8q22→ 8q24::18?::1q?q?). In 10 cases of MDS, 37 kinds of structural rearrangements were detected by M-FISH including insertion, deletion, translocation and derivative chromosomes, and among them 34 kinds were unbalanced rearrangements, only 3 were balanced rearrangements including t(6;22)(q21;q12), t(9;19)(q13;p13) and t(3;5)( ?;?), 7 abnormalities were never reported before. The CCAs invloved nearly all chromosomes, of which the chromosome 17, 5 and 7 were invloved more frequent than the rest. Chromosomes 5, 17, 7 were involved in 15 cases (62.5%), 12 cases (50%) and 6 cases (25%) respecrively. We conclude that M-FISH could refine CCAs of AML and MDS patients, find or correct the missed or misidentified aberrations by CC analysis. Our findings confirm that M-FISH is a powerful tool to characterize complex karyotypes in AML and MDS.

Genetic alterations of chromosome 17 in human breast carcinoma studied by fluorescence in situ hybridization and molecular DNA techniques

1994 ◽  
Vol 75 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Carla Rosenberg ◽  
Tone Ikdahl Andersen ◽  
Jahn Marthin Nesland ◽  
Mette Eknaes Lier ◽  
Anton Brøgger ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Breast Carcinoma ◽  
Fluorescence In Situ Hybridization ◽  
Genetic Alterations ◽  
Human Breast Carcinoma ◽  
Chromosome 17 ◽  
Human Breast

scholarly journals Reply: Diagnostic Utility of Chromosome 17 and p16 Abnormalities in Fluorescence In Situ Hybridization Tests in Primary Sclerosing Cholangitis

Hepatology ◽  
2010 ◽  
Vol 52 (1) ◽  
pp. 394-395
Author(s):  
Sanjay Y. Bangarulingam ◽  
Einar Björnsson ◽  
Felicity Enders ◽  
Emily G. Barr Fritcher ◽  
Gregory Gores ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Primary Sclerosing Cholangitis ◽  
Sclerosing Cholangitis ◽  
Chromosome 17 ◽  
Diagnostic Utility

scholarly journals Clinicopathologic features of breast cancer reclassified as HER2-amplified by fluorescence in situ hybridization with alternative chromosome 17 probes

2020 ◽  
Vol 48 ◽  
pp. 151576
Author(s):  
Kristen C. Blanton ◽  
Allison M. Deal ◽  
Kathleen A. Kaiser-Rogers ◽  
Carey K. Anders ◽  
Siobhan M. O'Connor ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome 17 ◽  
Clinicopathologic Features

Evaluation of chromosome 17 aneuploidy and p53 loss in endometriosis by fluorescence in situ hybridization (FISH).

2001 ◽  
Vol 76 (3) ◽  
pp. S45
Author(s):  
F.Z Bischoff ◽  
M.J Heard ◽  
D.X Dang ◽  
S.A Carson ◽  
J.E Buster ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome 17

Fluorescence in situ hybridization analysis of allelic losses involving the long arm of chromosome 17 in NF1-associated neurofibromas

2004 ◽  
Vol 150 (2) ◽  
pp. 168-172 ◽  
Author(s):  
Alessandro De Luca ◽  
Laura Bernardini ◽  
Caterina Ceccarini ◽  
Lorenzo Sinibaldi ◽  
Antonio Novelli ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Hybridization Analysis ◽  
Chromosome 17

Evaluation of HER-2/neu Gene Status in Osteosarcoma by Fluorescence In Situ Hybridization and Multiplex and Monoplex Polymerase Chain Reactions

2006 ◽  
Vol 130 (5) ◽  
pp. 691-698 ◽  
Author(s):  
Carlynn Willmore-Payne ◽  
Joseph A. Holden ◽  
Holly Zhou ◽  
Dilip Gupta ◽  
Sharon Hirschowitz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Gene Amplification ◽  
Chromosome 17 ◽  
Fish Analysis ◽  
Chain Reaction ◽  
Her 2 ◽  
Polymerase Chain

Abstract Context.—Previous reports suggest that the human epidermal growth factor 2 (HER-2/neu) receptor may be overexpressed in osteosarcoma. Objective.—To determine whether osteosarcomas have amplifications of the HER-2/neu gene. Design.—We studied a series of osteosarcomas by fluorescence in situ hybridization (FISH) and by 2 real-time polymerase chain reaction assays that measure the amount of HER-2/neu DNA relative to a control gene. The HER-2/ neu monoplex and multiplex assays were capable of identifying those cases of breast cancer that were known to overexpress HER-2/neu as assessed by FISH. We initially studied 21 cases of osteosarcoma by FISH analysis (using a technique that included a probe for chromosome 17), 11 of which had their HER-2/neu gene amplification status previously reported. Results.—None of these osteosarcoma cases showed HER-2/neu amplification by our FISH analysis and subsequent quantitative (multiplex) polymerase chain reaction. Apparent expression of HER-2/neu protein was observed in several of the cases but the immunoreactivity was localized to the cytoplasm and was not membranous in character. An additional 35 osteosarcoma specimens were subjected to monoplex polymerase chain reaction analysis, and amplifiable DNA was recovered from 19 specimens (54%). None of these samples had HER-2/neu amplification by monoplex PCR analysis and only one case had membranous immunoreactivity graded as 1+. Conclusion.—Although a small subset of osteosarcomas had weak noncircumferential membranous immunoreactivity for HER-2/neu protein, no osteosarcomas demonstrated positive (2+ or 3+) immunoreactivity for HER-2/ neu protein and none showed HER-2/neu gene amplification by either FISH or polymerase chain reaction.

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