Lactoferrin Expression by Bovine Ocular Surface Epithelia: A Primary Cell Culture Model to Study Lactoferrin Gene Promoter Activity

2005 ◽  
Vol 37 (5) ◽  
pp. 270-278 ◽  
Author(s):  
Maria Grazia Santagati ◽  
Simona La Terra Mulè ◽  
Carla Amico ◽  
Matteo Pistone ◽  
Dario Rusciano ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ocular Surface ◽  
Promoter Activity ◽  
Primary Cell Culture ◽  
Gene Promoter ◽  
Primary Cell ◽  
Cell Culture Model ◽  
Culture Model ◽  
Lactoferrin Gene

scholarly journals Subretinal Pigment Epithelial Deposition of Drusen Components Including Hydroxyapatite in a Primary Cell Culture Model

2017 ◽  
Vol 58 (2) ◽  
pp. 708 ◽  
Author(s):  
Matthew G. Pilgrim ◽  
Imre Lengyel ◽  
Antonio Lanzirotti ◽  
Matt Newville ◽  
Sarah Fearn ◽  
...  
Keyword(s):  
Cell Culture ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Cell Culture Model ◽  
Pigment Epithelial ◽  
Culture Model

A primary cell culture model for defective cardiac myofibrillogenesis in mexican axolotl embryos

1997 ◽  
Vol 33 (9) ◽  
pp. 677-680
Author(s):  
Robert W. Zajdel ◽  
Yongze Zhu ◽  
Margaret E. Fransen ◽  
Larry F. Lemanski
Keyword(s):  
Cell Culture ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Cell Culture Model ◽  
Mexican Axolotl ◽  
Culture Model

Hyperphosphorylation of MEF2A in primary adipocytes correlates with downregulation of human GLUT4 gene promoter activity

2007 ◽  
Vol 292 (4) ◽  
pp. E1149-E1156 ◽  
Author(s):  
David P. Sparling ◽  
Beth A. Griesel ◽  
Ann Louise Olson
Keyword(s):  
Cell Culture ◽  
Transcription Factor ◽  
Promoter Activity ◽  
Model Systems ◽  
Regulatory Control ◽  
Cell Culture Model ◽  
In Vitro System ◽  
Culture Model ◽  
Enhancer Factor

GLUT4 promoter activity is regulated by hormonal, metabolic, and tissue-specific controls. This complicates the study of GLUT4 gene transcription, as no cell culture model adequately recapitulates these extracellular regulators. While investigating cultured primary adipocytes as a model system for GLUT4 transcription, we observed that GLUT4 mRNA was specifically and rapidly downregulated upon tissue dispersal. Downregulation of GLUT4 mRNA was mediated in part by loss of regulatory control by the trans-acting factors that control GLUT4 transcriptional activity [the myocyte enhancer factor 2 (MEF2) transcription factor family and the GLUT4 enhancer factor] and their cognate DNA binding sites in transgenic mice. The differences in GLUT4 transcription when whole adipose tissue and cell culture model systems are compared can be correlated to a posttranslational phosphorylation of the transcription factor MEF2A. The difference in the MEF2A phosphorylation state in whole tissue vs. isolated cells may provide a further basis for the development of an in vitro system that could recapitulate fully regulated GLUT4 promoter activity. Development of an in vitro system to reconstitute GLUT4 transcriptional regulation will further efforts to discern the molecular mechanisms that underlie GLUT4 expression.

1120: Citrate and Vitamin E Blunt the SWL-Induced Free Radical Surge in an In-Vitro MDCK Cell Culture Model

2004 ◽  
Vol 171 (4S) ◽  
pp. 295-295
Author(s):  
Fernando C. Delvecchio ◽  
Ricardo M. Brizuela ◽  
Karen J. Byer ◽  
W. Patrick Springhart ◽  
Saeed R. Khan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Free Radical ◽  
Vitamin E ◽  
Mdck Cell ◽  
Cell Culture Model ◽  
Culture Model
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