Decreased Production of Collagen Type III in Cultured Smooth Muscle Cells from Varicose Vein Patients Is due to a Degradation by MMPs: Possible Implication of MMP-3

2005 ◽  
Vol 42 (5) ◽  
pp. 388-398 ◽  
Author(s):  
Patricia Sansilvestri-Morel ◽  
Alain Rupin ◽  
Nicolas D. Jullien ◽  
Nathalie Lembrez ◽  
Patricia Mestries-Dubois ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Varicose Vein ◽  
Collagen Type ◽  
Muscle Cells ◽  
Collagen Type Iii ◽  
Type Iii ◽  
Cultured Smooth Muscle Cells

Imbalance in the Synthesis of Collagen Type I and Collagen Type III in Smooth Muscle Cells Derived from Human Varicose Veins

2001 ◽  
Vol 38 (6) ◽  
pp. 560-568 ◽  
Author(s):  
Patricia Sansilvestri-Morel ◽  
Alain Rupin ◽  
Cécile Badier-Commander ◽  
Patrick Kern ◽  
Jean-Noël Fabiani ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Collagen Type ◽  
Varicose Veins ◽  
Muscle Cells ◽  
Collagen Type I ◽  
Type I ◽  
Collagen Type Iii ◽  
Type Iii

A Novel In-Vitro System for the Simultaneous Exposure of Bladder Smooth Muscle Cells to Mechanical Strain and Sustained Hydrostatic Pressure

2002 ◽  
Vol 124 (2) ◽  
pp. 208-213 ◽  
Author(s):  
Karen M. Haberstroh ◽  
Martin Kaefer ◽  
Natacha DePaola ◽  
Sarah A. Frommer ◽  
Rena Bizios
Keyword(s):  
Smooth Muscle ◽  
Hydrostatic Pressure ◽  
Smooth Muscle Cells ◽  
Collagen Type ◽  
Mechanical Strain ◽  
Muscle Cells ◽  
Collagen Type Iii ◽  
Bladder Smooth Muscle ◽  
Simultaneous Exposure

The novel hydrostrain system was designed in an effort to establish and maintain conditions that simulate the in-vivo mechanical environment of the bladder. In this laboratory system, ovine bladder smooth muscle cells on flexible, 10-cm-dia silastic membranes were exposed simultaneously to hydrostatic pressure (40 cm H2O, a pressure level currently associated with bladder pathologies) and mechanical strains (up to 25 percent) under standard cell culture conditions for 7 h. Under these conditions, Heparin Binding-Epidermal Growth Factor and Collagen Type III mRNA expression were significantly increased (p<0.01 and 0.1, respectively); however, no changes were observed in Collagen Type I mRNA expression. Decreases in the Collagen Type I:Type III ratio following simultaneous exposure of bladder smooth muscle cells to pathological levels of hydrostatic pressure and mechanical strain in vitro are in agreement with clinically observed increases in Collagen Type III with concomitant decreased human bladder compliance. The results of the present study, therefore, provide cellular/molecular level information relevant to bladder pathology that could have significant implications in the field of clinical urology.

Autocrine Induction of DNA Synthesis by Mechanical Injury of Cultured Smooth Muscle Cells

1996 ◽  
Vol 16 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Federico Calara ◽  
Sean Ameli ◽  
Anna Hultgårdh-Nilsson ◽  
Bojan Cercek ◽  
Joel Kupfer ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Muscle Cells ◽  
Mechanical Injury ◽  
Cultured Smooth Muscle Cells

Abnormal Deposition of Extracellular Matrix Proteins by Cultured Smooth Muscle Cells from Human Varicose Veins

1998 ◽  
Vol 35 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Patricia Sansilvestri-Morel ◽  
Isabelle Nonotte ◽  
Marie-Pierre Fournet-Bourguignon ◽  
Alain Rupin ◽  
Jean-Noël Fabiani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Varicose Veins ◽  
Extracellular Matrix Proteins ◽  
Muscle Cells ◽  
Matrix Proteins ◽  
Cultured Smooth Muscle Cells

An ATP-sensitive conductance in cultured smooth muscle cells from pregnant rat myometrium

1989 ◽  
Vol 257 (2) ◽  
pp. C297-C305 ◽  
Author(s):  
E. Honore ◽  
C. Martin ◽  
C. Mironneau ◽  
J. Mironneau
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Voltage Clamp ◽  
Free Acid ◽  
Muscle Cells ◽  
Monovalent Cation ◽  
Acid Form ◽  
Pregnant Rat ◽  
Free Acid Form ◽  
Cultured Smooth Muscle Cells

The whole cell voltage-clamp technique was used to study the effects of extracellular ATP in cultured smooth muscle cells isolated from pregnant rat myometrium. An inward current was elicited by ATP (IATP) in cells held at -70 mV under voltage clamp. The amplitude of IATP was reduced by estrogen pretreatment and by the end of pregnancy. IATP not only did not undergo any desensitization but showed facilitation. The current-voltage relationship of IATP was linear and reversed close to 0 mV. Changing the sodium electrochemical gradient by decreasing extracellular or intracellular sodium resulted in a linear relationship between the reversal potential of IATP and Na equilibrium potential that, however, differed from the predicted curve for a purely sodium conductance. The conductance activated by ATP was monovalent cation selective with little discrimination between potassium, cesium, and sodium ions. IATP was depressed by divalent cations, and the rank order of potency was Co greater than Mg greater than Ca greater than Ba, suggesting that the free-acid form of ATP was the effective ligand. Adenosine, AMP, and ADP were ineffective in eliciting IATP, whereas ATP gamma S and alpha,beta-methylene ATP were capable of mimicking the effects of ATP, although they were less potent. These results are consistent with the free-acid form of ATP activating a monovalent cation-selective and estrogen-sensitive conductance in myometrium.

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