scholarly journals Characterization of Novel Airway Submucosal Gland Cell Models for Cystic Fibrosis

2005 ◽  
Vol 15 (6) ◽  
pp. 251-262 ◽  
Author(s):  
Ana da Paula ◽  
Anabela Ramalho ◽  
Carlos Farinha ◽  
Judy Cheung ◽  
Rosalie Maurisse ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gland Cell ◽  
Cell Models ◽  
Submucosal Gland

scholarly journals Functional insertion of the SV40 large T oncogene in cystic fibrosis intestinal epithelium. Characterization of CFI-3 cells.

1991 ◽  
Vol 266 (31) ◽  
pp. 21239-21246
Author(s):  
E. Chastre ◽  
Y. Di Gioia ◽  
P. Barbry ◽  
B. Simon-Bouy ◽  
E. Mornet ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Intestinal Epithelium

scholarly journals Molecular Characterization of Achromobacter Isolates from Cystic Fibrosis and Non-Cystic Fibrosis Patients in Madrid, Spain

2013 ◽  
Vol 51 (6) ◽  
pp. 1927-1930 ◽  
Author(s):  
L. Barrado ◽  
P. Branas ◽  
M. A. Orellana ◽  
M. T. Martinez ◽  
G. Garcia ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Molecular Characterization

scholarly journals Genomic, Tissue Expression, and Protein Characterization of pCLCA1, a Putative Modulator of Cystic Fibrosis in the Pig

2009 ◽  
Vol 57 (12) ◽  
pp. 1169-1181 ◽  
Author(s):  
Stephanie Plog ◽  
Lars Mundhenk ◽  
Nikolai Klymiuk ◽  
Achim D. Gruber
Keyword(s):  
Cystic Fibrosis ◽  
Tissue Expression ◽  
Protein Characterization

Cystic Fibrosis: Characterization of the Inhibitor to Ciliary Action in Oyster Gills

Science ◽  
1970 ◽  
Vol 167 (3919) ◽  
pp. 871-873 ◽  
Author(s):  
B. H. Bowman ◽  
M. L. McCombs ◽  
L. H. Lockhart
Keyword(s):  
Cystic Fibrosis ◽  
Ciliary Action

CFTR involvement in chloride, bicarbonate, and liquid secretion by airway submucosal glands

1999 ◽  
Vol 277 (4) ◽  
pp. L694-L699 ◽  
Author(s):  
Stephen T. Ballard ◽  
Laura Trout ◽  
Zsuzsa Bebök ◽  
E. J. Sorscher ◽  
Angela Crews
Keyword(s):  
Cystic Fibrosis ◽  
Channel Blocker ◽  
Polyclonal Antibodies ◽  
Surface Epithelium ◽  
Transmembrane Conductance Regulator ◽  
Submucosal Gland ◽  
Transmembrane Conductance ◽  
Active Secretion ◽  
Submucosal Glands ◽  
Cystic Fibrosis Transmembrane

Previous studies demonstrated that ACh-induced liquid secretion by porcine bronchi is driven by active Cl− and H[Formula: see text] secretion. The present study was undertaken to determine whether this process was localized to submucosal glands and mediated by the cystic fibrosis transmembrane conductance regulator (CFTR). When excised, cannulated, and treated with ACh, porcine bronchi secreted 15.6 ± 0.6 μl ⋅ cm−2 ⋅ h−1. Removal of the surface epithelium did not significantly affect the rate of secretion, indicating that the source of the liquid was the submucosal glands. Pretreatment with diphenylamine-2-carboxylate, a relatively nonselective Cl−-channel blocker, significantly reduced liquid secretion by 86%, whereas pretreatment with DIDS, which inhibits a variety of Cl− channels but not CFTR, had no effect. When bronchi were pretreated with glibenclamide or 5-nitro-2-(3-phenylpropylamino)benzoic acid (both inhibitors of CFTR), the rate of ACh-induced liquid secretion was significantly reduced by 39 and 91%, respectively, compared with controls. Agents that blocked liquid secretion also caused disproportionate reductions in H[Formula: see text] secretion. Polyclonal antibodies to the CFTR bound preferentially to submucosal gland ducts and the surface epithelium, suggesting that this channel was localized to these sites. These data suggest that ACh-induced gland liquid secretion by porcine bronchi is driven by active secretion of both Cl− and H[Formula: see text] and is mediated by the CFTR.

A characterization of the ZFL cell line and primary hepatocytes as in vitro liver cell models for the zebrafish (Danio rerio)

2014 ◽  
Vol 147 ◽  
pp. 7-17 ◽  
Author(s):  
Marta Eide ◽  
Marte Rusten ◽  
Rune Male ◽  
Knut Helge Midtbø Jensen ◽  
Anders Goksøyr
Keyword(s):  
Cell Line ◽  
Danio Rerio ◽  
Liver Cell ◽  
Cell Models ◽  
Primary Hepatocytes
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