Corneal Cell Density Measurement in vivo by Scanning Slit Confocal Microscopy: Method and Validation

2004 ◽  
Vol 36 (5) ◽  
pp. 270-276 ◽  
Author(s):  
Mónika Popper ◽  
António Miguel Morgado ◽  
Maria João Quadrado ◽  
Jaap A. van Best
Keyword(s):  
Confocal Microscopy ◽  
Cell Density ◽  
Density Measurement ◽  
Corneal Cell ◽  
Microscopy Method

scholarly journals In Vivo Confocal Microscopy Shows Alterations in Nerve Density and Dendritiform Cell Density in Fuchs’ Endothelial Corneal Dystrophy

2018 ◽  
Vol 196 ◽  
pp. 136-144 ◽  
Author(s):  
Shruti Aggarwal ◽  
Bernardo M. Cavalcanti ◽  
Laura Regali ◽  
Andrea Cruzat ◽  
Monique Trinidad ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Cell Density ◽  
Corneal Dystrophy ◽  
In Vivo Confocal Microscopy

Diabetes and Corneal Cell Densities in Humans by In Vivo Confocal Microscopy

Cornea ◽  
2006 ◽  
Vol 25 (7) ◽  
pp. 761-768 ◽  
Author(s):  
Maria João Quadrado ◽  
Monika Popper ◽  
António Miguel Morgado ◽  
Joaquim Neto Murta ◽  
Jaap A Van Best
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy ◽  
Corneal Cell ◽  
Cell Densities

Corneal epithelial dendritic cell density in the healthy human cornea: A meta-analysis of in-vivo confocal microscopy data

2019 ◽  
Vol 17 (4) ◽  
pp. 753-762 ◽  
Author(s):  
Rabia Mobeen ◽  
Fiona Stapleton ◽  
Cecilia Chao ◽  
Michele C. Madigan ◽  
Nancy Briggs ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Confocal Microscopy ◽  
Cell Density ◽  
Meta Analysis ◽  
Human Cornea ◽  
In Vivo Confocal Microscopy ◽  
Healthy Human ◽  
Corneal Epithelial ◽  
Microscopy Data

scholarly journals Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome

Diagnostics ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 497 ◽  
Author(s):  
Yukihiro Matsumoto ◽  
Osama M. A. Ibrahim ◽  
Takashi Kojima ◽  
Murat Dogru ◽  
Jun Shimazaki ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Cell Density ◽  
Laser Scanning ◽  
Inflammatory Cell ◽  
Laser Scanning Confocal Microscopy ◽  
Fiber Density ◽  
Scanning Confocal Microscopy ◽  
Control Subjects ◽  
Nerve Fiber Density

Purpose: To evaluate the changes in cornea in Sjögren’s syndrome (SS) with a novel confocal microscopy device. Methods: Twenty-three right eyes of patients with SS (23 women; mean age, 65.4 ± 11.4 years) and 13 right eyes of 13 age- and sex-matched control subjects (13 women; mean age, 68.8 ± 9.8 years) were studied. Furthermore, eight right eyes of patients with SS (8 women; mean age, 66.9 ± 9.6 years) were studied to evaluate the corneal microscopic alterations after the treatment with topical 3% diquafosol sodium eye drops. All cases had tear quantity, tear breakup time (BUT), ocular surface staining measurements, and corneal in vivo laser-scanning confocal microscopy examinations. The density and area of corneal epithelial cells (superficial, wing, and basal), density of corneal stromal cells (anterior, intermediate, and posterior), density and area of corneal endothelial cells, density and morphology of corneal sub-basal nerve plexus, density of corneal sub-basal inflammatory cells were also assessed. Results: The tear quantity, stability, and vital staining scores were significantly worse in patients with SS than in control subjects (p < 0.0001). Corneal superficial epithelial cell density was significantly lower in SS compared with control subjects (p < 0.0001). Corneal superficial epithelial cell area was significantly larger in SS compared with control subjects (p = 0.007). Corneal sub-basal nerve fiber density was lower in SS compared with control subjects (p < 0.0001). Morphological abnormality of nerve fibers was observed in SS patients. Corneal sub-basal inflammatory cell density was significantly higher in SS patients compared with control subjects (p < 0.0001). Furthermore, the mean corneal superficial epithelial cell density and area, inflammatory cell density, corneal sub-basal nerve fiber density, and morphological abnormality of nerve fibers, were improved with topical 3% diquafosol sodium treatment in the dry eye patients with SS (p < 0.05). Conclusions: The diagnostic modality using in vivo laser-scanning confocal microscopy was a useful method for the evaluation of the corneal cell density and area, nerve fiber density and morphology, and inflammatory cell density in patients with SS and also a useful tool in the assessment of treatment effect with topical 3% diquafosol sodium in the SS patients.

scholarly journals The influence of pseudoexfoliative syndrome on corneal morphology based on in vivo confocal microscopy

2017 ◽  
Vol 10 (2) ◽  
pp. 49-55 ◽  
Author(s):  
Vitaly V Potemkin ◽  
Tatyana S Varganova ◽  
Evgeniy L Akopov ◽  
Elena V Ageeva
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Real Time ◽  
Cell Density ◽  
In Vivo Imaging ◽  
Morphological Changes ◽  
In Vivo Confocal Microscopy ◽  
Examination Method ◽  
Mode A

Confocal microscopy is a modern examination method, which provides in real-time mode a noninvasive in vivo imaging of the cornea, limb, and conjunctiva. Purpose. To evaluate main morphological changes observed by confocal microscopy in patients with pseudoexfoliation (PEX) syndrome. Methods. 21 patients were examined. 12 patients with PEX syndrome were enrolled as the main group, and 9 patients without PEX - as controls. Results. In patients with PEX, there was a decreased cell density in the epithelium and the stroma of the cornea, as well as a lot of hyperreflective intercellular microdeposits and dendritic cells (p < 0.05).

In vivo confocal microscopy indicates an inverse relationship between the sub-basal corneal plexus and the conjunctivalisation in patients with limbal stem cell deficiency

2018 ◽  
Vol 103 (3) ◽  
pp. 327-331 ◽  
Author(s):  
Manuel Caro-Magdaleno ◽  
Asunción Alfaro-Juárez ◽  
Jesús Montero-Iruzubieta ◽  
Ana Fernández-Palacín ◽  
Ana Muñoz-Morales ◽  
...  
Keyword(s):  
Stem Cell ◽  
Confocal Microscopy ◽  
Cell Density ◽  
Sensitivity And Specificity ◽  
Basal Cell ◽  
Limbal Stem Cell Deficiency ◽  
Nerve Plexus ◽  
In Vivo Confocal Microscopy ◽  
Limbal Stem Cell

Background/aimsLimbal stem cell deficiency (LSCD) is characterised by a marked decrease in limbal stem cells. It is classified primarily using subjective slit-lamp observations. In vivo confocal microscopy (IVCM) can non-invasively provide objective information on the condition of the limbal niche, the corneal epithelial basal cell density and the corneal sub-basal nerve plexus density (SND). We here used IVCM to evaluate changes in SND to improve LSCD classification.MethodsWe evaluated and classified 38 patients (76 eyes, 44 with LSC and 32 control eyes) using the Rama, López-García and Deng (clinical and confocal) classifications and evaluated the concordance of the confocal and clinical classifications. We constructed a logistic regression model using multivariate analysis to correlate different degrees of conjunctivalisation with IVCM parameters and used receiver operating characteristic (ROC) curve analysis to establish the SND cut-off value with maximum diagnostic sensitivity and specificity.ResultsThe classification systems correlated moderately at best (kappa, 0.449). The corneal SND of cases (6469±6295 µm/mm2) was less (p<0.001) than in controls (20911±4142 µm/mm2). The SND, but not basal cell density, played a protective role against conjunctivalisation (OR, 0.069; 95% CI 0.008–0.619; p=0.01). An SND cut-off value of 17 215 µm/mm2 yielded a sensitivity and specificity of 95.5% and 90.6%, respectively, for LSCD diagnosis.ConclusionThe density of the corneal sub-basal nerve plexus was inversely related to conjunctivalisation in LSCD. Further studies are needed to verify this and to elucidate the directionality between these factors.

Normal Human Corneal Cell Populations Evaluated by In Vivo Scanning Slit Confocal Microscopy

Cornea ◽  
1998 ◽  
Vol 17 (5) ◽  
pp. 485-492 ◽  
Author(s):  
Raine K. Mustonen ◽  
Marguerite B. McDonald ◽  
Sabong Srivannaboon ◽  
Alnette L. Tan ◽  
Mark W. Doubrava ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Cell Populations ◽  
Corneal Cell ◽  
Normal Human
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