Ethanol-Induced Changes in Lipid Composition of Intestinal Microvillus Membrane in Rats Fed Different Dietary Fats

2004 ◽  
Vol 48 (4) ◽  
pp. 221-227 ◽  
Author(s):  
Meenu Kaur ◽  
Jyotdeep Kaur ◽  
Reena Gupta ◽  
Sudarshan Ojha ◽  
Akhtar Mahmood
Keyword(s):  
Lipid Composition ◽  
Dietary Fats ◽  
Microvillus Membrane ◽  
Induced Changes

Impaired alanine uptake by basolateral liver plasma membrane vesicles from rats consuming ethanol

1991 ◽  
Vol 261 (6) ◽  
pp. G913-G920
Author(s):  
D. J. Smith ◽  
S. A. Ploch
Keyword(s):  
Plasma Membrane ◽  
Lipid Composition ◽  
Cholesterol Content ◽  
Ethanol Consumption ◽  
Chronic Ethanol ◽  
Transport Systems ◽  
Liver Plasma Membrane ◽  
Alanine Transport ◽  
Chronic Ethanol Consumption ◽  
Induced Changes

Chronic ethanol consumption reduces alanine transport by rat basolateral liver plasma membrane (blLPM) vesicles; however, the mechanism for this effect remains uncertain. It may be related to the ethanol-induced changes in blLPM fluidity and lipid composition; alternatively ethanol might reduce the number of transporters in the blLPM. To investigate the effect of blLPM fluidity and lipid composition on Na(+)-dependent alanine uptake these parameters were altered in vitro. Increasing the blLPM fluidity had no effect on Na(+)-dependent alanine uptake by blLPM vesicles or the activity of amino acid transport systems, A and ASC. Because ethanol is known to reduce the blLPM cholesterol content, the influence of altering blLPM cholesterol on alanine transport by these membranes was investigated next. Neither an increase nor a decrease of the cholesterol content of the blLPM altered Na(+)-dependent alanine uptake or the activity of system A or ASC. Finally, the influence of chronic ethanol consumption on the specific binding of [3H]alanine to blLPM was studied. The dissociation constant for alanine binding to blLPM from ethanol-fed rats and their pair-fed controls was similar (1.9 +/- 0.2 vs. 2.0 +/- 0.3 mM); however, the maximal binding capacity for alanine was significantly (P less than 0.05) lower in the blLPM from ethanol-fed rats (316 +/- 53 pmol/mg protein) compared with their pair-fed controls (527 +/- 79 pmol/mg protein). These studies do not support the hypothesis that ethanol-induced changes in blLPM fluidity are responsible for the impaired alanine transport; they do suggest that ethanol may reduce the

scholarly journals Lipid composition of the isolated rat intestinal microvillus membrane

1968 ◽  
Vol 109 (1) ◽  
pp. 51-59 ◽  
Author(s):  
G. G. Forstner ◽  
K. Tanaka ◽  
K. J. Isselbacher
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Composition ◽  
Plasma Membranes ◽  
Membrane Lipids ◽  
Saturated Fatty Acids ◽  
Polar Lipids ◽  
Published Data ◽  
Microvillus Membrane

1. Rat intestinal microvillus plasma membranes were prepared from previously isolated brush borders and the lipid composition was analysed. 2. The molar ratio of cholesterol to phospholipid was greatest in the membranes and closely resembled that reported for myelin. 3. Unesterified cholesterol was the major neutral lipid. However, 30% of the neutral lipid fraction was accounted for by glycerides and fatty acid. 4. Five phospholipid components were identified and measured, including phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, sphingomyelin and lysophosphatidylcholine. Though phosphatidylethanolamine was the chief phospholipid, no plasmalogen was detected. 5. In contrast with other plasma membranes in the rat, the polar lipids of the microvillus membrane were rich in glycolipid. The cholesterol:polar lipid (phospholipid+glycolipid) ratio was about 1:3 for the microvillus membrane. Published data suggest that this ratio resembles that of the liver plasma membrane more closely than myelin or the erythrocyte membrane. 6. The fatty acid composition of membrane lipids was altered markedly by a single feeding of safflower oil. Membrane polar lipids did not contain significantly more saturated fatty acids than cellular polar lipids. Differences in the proportion of some fatty acids in membrane and cellular glycerides were noted. These differences may reflect the presence of specific membrane glycerides.

Detergent induced changes in serum lipid composition in rats

Lipids ◽  
1989 ◽  
Vol 24 (11) ◽  
pp. 915-918 ◽  
Author(s):  
Yoshiro Miura ◽  
Harumi Hisaki ◽  
Benzo Fukushima ◽  
Tatsuo Nagai ◽  
Tohru Ikeda
Keyword(s):  
Lipid Composition ◽  
Serum Lipid ◽  
Induced Changes

Effects of Dietary Fats on Lipid Composition of Rabbit Molar Dentin

1978 ◽  
Vol 57 (2) ◽  
pp. 420-422 ◽  
Author(s):  
S.K. Das ◽  
D.K. Bhattacharyya
Keyword(s):  
Lipid Composition ◽  
Dietary Fats
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