Role of Isoprenoids in Cytoskeleton Integrity and Albumin Endocytosis by Opossum Kidney Cells

2004 ◽  
Vol 97 (3) ◽  
pp. e77-e85 ◽  
Author(s):  
Steven J. McTaggart ◽  
Eveline Eitle ◽  
Peter J. Harris ◽  
Colin L. Jones
Keyword(s):  
Kidney Cells ◽  
Opossum Kidney ◽  
Opossum Kidney Cells ◽  
Cytoskeleton Integrity ◽  
Albumin Endocytosis

scholarly journals Ouabain-insensitive acidification by dopamine in renal OK cells: primary control of the Na+/H+exchanger

2001 ◽  
Vol 281 (1) ◽  
pp. R10-R18 ◽  
Author(s):  
Pedro Gomes ◽  
M. A. Vieira-Coelho ◽  
P. Soares-da-Silva
Keyword(s):  
Adenylyl Cyclase ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Kidney Cells ◽  
Primary Control ◽  
Intracellular Acidification ◽  
Opossum Kidney ◽  
Opossum Kidney Cells ◽  
Stimulation Of

The present study was aimed at evaluating the role of D1- and D2-like receptors and investigating whether inhibition of Na+ transepithelial flux by dopamine is primarily dependent on inhibition of the apical Na+/H+ exchanger, inhibition of the basolateral Na+-K+-ATPase, or both. The data presented here show that opossum kidney cells are endowed with D1- and D2-like receptors, the activation of the former, but not the latter, accompanied by stimulation of adenylyl cyclase (EC50 = 220 ± 2 nM), marked intracellular acidification (IC50 = 58 ± 2 nM), and attenuation of amphotericin B-induced decreases in short-circuit current (28.6 ± 4.5% reduction) without affecting intracellular pH recovery after CO2 removal. These results agree with the view that dopamine, through the activation of D1- but not D2-like receptors, inhibits both the Na+/H+ exchanger (0.001933 ± 0.000121 vs. 0.000887 ± 0.000073 pH unit/s) and Na+-K+-ATPase without interfering with the Na+-independent HCO[Formula: see text] transporter. It is concluded that dopamine, through the action of D1-like receptors, inhibits both the Na+/H+ exchanger and Na+-K+-ATPase, but its marked acidifying effects result from inhibition of the Na+/H+exchanger only, without interfering with the Na+-independent HCO[Formula: see text] transporter and Na+-K+-ATPase.

scholarly journals Role of membrane microdomains in PTH-mediated down-regulation of NaPi-IIa in opossum kidney cells

2005 ◽  
Vol 68 (3) ◽  
pp. 1137-1147 ◽  
Author(s):  
Kunitaka Nashiki ◽  
Yutaka Taketani ◽  
Tomoko Takeichi ◽  
Naoki Sawada ◽  
Hironori Yamamoto ◽  
...  
Keyword(s):  
Membrane Microdomains ◽  
Kidney Cells ◽  
Down Regulation ◽  
Opossum Kidney ◽  
Opossum Kidney Cells
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