Resistance of Cultured Human Skin Fibroblasts from Old and Young Donors to Oxidative Stress and Their Glutathione Peroxidase Activity

Gerontology ◽  
2004 ◽  
Vol 50 (4) ◽  
pp. 193-199 ◽  
Author(s):  
Mitsuyoshi Matsuo ◽  
Hidefumi Ikeda ◽  
Takehiko Sugihara ◽  
Satomi Horiike ◽  
Yuri Okano ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Human Skin ◽  
Peroxidase Activity ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Glutathione Peroxidase Activity

scholarly journals Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Itana Gomes Alves Andrade ◽  
Fabíola Isabel Suano-Souza ◽  
Fernando Luiz Affonso Fonseca ◽  
Carolina Sanchez Aranda Lago ◽  
Roseli Oselka Saccardo Sarni
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Oxidized Ldl ◽  
Reference Value ◽  
Glutathione Peroxidase Activity ◽  
Oxidative Stress Biomarkers ◽  
Apo B ◽  
Lipid Status ◽  
And Oxidative Stress

Abstract Introduction Ataxia-Telangiectasia (A-T) is a multi-system disorder that may be associated with endocrine changes, oxidative stress in addition to inflammation. Studies suggest that selenium is a trace element related to protection against damage caused by oxidative stress. Objective To describe the plasma levels of selenium and erythrocyte glutathione peroxidase activity in A-T patients and to relate them to oxidative stress and lipid status biomarkers. Methods This is a cross-sectional and controlled study evaluating 22 A-T patients (age median, 12.2 years old) matched by gender and age with 18 healthy controls. We evaluated: nutritional status, food intake, plasma selenium levels, erythrocyte glutathione peroxidase activity, lipid status, inflammation and oxidative stress biomarkers. Results Adequate levels of selenium were observed in 24/36 (66.7%) in this evaluated population. There was no statistically significant difference between the groups in selenium levels [47.6 μg/L (43.2–57.0) vs 54.6 (45.2–62.6) μg/dL, p = 0.242]. Nine of A-T patients (41%) had selenium levels below the reference value. The A-T group presented higher levels of LDL-c, non-HDL-c, oxidized LDL, Apo B, Apo-B/Apo-A-I1, LDL-c/HDL-c ratio, malondialdehyde [3.8 µg/L vs 2.8 µg/L, p = 0.029] and lower Apo-A-I1/HDL-c and glutathione peroxidase activity [7300 U/L vs 8686 U/L, p = 0.005]. Selenium levels were influenced, in both groups, independently, by the concentrations of oxidized LDL, malonaldehyde and non-HDL-c. The oxidized LDL (AUC = 0.849) and ALT (AUC = 0.854) were the variables that showed the greatest discriminatory power between groups. Conclusion In conclusion, we observed the presence of selenium below the reference value in nearly 40% and low GPx activity in A-T patients. There was a significant, inverse and independent association between selenium concentrations and oxidative stress biomarkers. Those data reinforce the importance of assessing the nutritional status of selenium in those patients.

scholarly journals OXIDATIVE STRESS MARKERS IN THE LUNGS UNDER EXPERIMENTAL HYPEROXIA WITH INHALED LIPOSOMES BASED ON EGG LECITHIN

Biomeditsina ◽  
2019 ◽  
pp. 49-58
Author(s):  
I. L. Kotovich ◽  
Zh. A. Rutkovskaya ◽  
A. D. Taganovich
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Alpha Tocopherol ◽  
Oxygen Species ◽  
Glutathione Peroxidase Activity ◽  
Diene Conjugates ◽  
Hyperoxia Exposure ◽  
Egg Lecithin

Oxidative stress is considered to be a factor leading to lung damage in premature infants. The aim of this study was to investigate the effect of inhaled antioxidants incorporated into egg lecithin liposomes on the indicators of oxidative stress in the lungs of newborn guinea pigs under experimental hyperoxia (3 days). Bronchoalveolar lavage fl uid (BALF) was used as a material for the study. Under hyperoxia exposure, inhalation of liposomes containing N-acetylcysteine and alpha-tocopherol contributed to the suppression of the reactive oxygen species production by cells, normalization of glutathione peroxidase activity and carbonyls content, while not affecting the level of diene conjugates in BALF. The introduction of retinoid-containing liposomes (retinol and retinoic acid) under hyperoxia was accompanied by normalization of glutathione peroxidase activity as well as the content of protein oxidation products in BALF, while the generation of reactive oxygen species remained enhanced, and the diene conjugates and thiobarbituric acid reactive products exceeded the levels in animals exposed to hyperoxia alone. Thus, the inhaled liposomes containing retinoids and egg lecithin exhibit not only anti-, but also a prooxidant effect in the lungs under hyperoxia exposure, unlike the liposomal forms of N-acetylcysteine and alpha-tocopherol.

scholarly journals Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI

2019 ◽  
Vol 42 (1) ◽  
pp. 1-8 ◽  
Author(s):  
José Araújo de Oliveira-Silva ◽  
Joyce Umbelino Pinto Yamamoto ◽  
Renata Bernardes de Oliveira ◽  
Vaneisse Cristina Lima Monteiro ◽  
Beatriz Jurkiewcz Frangipani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Selenium Supplementation ◽  
Glutathione Peroxidase Activity ◽  
Stress Assessment ◽  
Mucopolysaccharidosis I

Silver ions induce oxidative stress and intracellular zinc release in human skin fibroblasts

2009 ◽  
Vol 47 (11) ◽  
pp. 1570-1577 ◽  
Author(s):  
Miriam M. Cortese-Krott ◽  
Meike Münchow ◽  
Elvis Pirev ◽  
Florian Heβner ◽  
Ahmed Bozkurt ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Skin ◽  
Silver Ions ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Intracellular Zinc ◽  
Zinc Release

Stimulation of Glutathione Peroxidase Activity Decreases HIV Type 1 Activation after Oxidative Stress

1994 ◽  
Vol 10 (11) ◽  
pp. 1451-1461 ◽  
Author(s):  
CHRISTINE SAPPEY ◽  
SYLVIE LEGRAND-POELS ◽  
MARTIN BEST-BELPOMME ◽  
ALAIN FAVIER ◽  
BERNARD RENTIER ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Glutathione Peroxidase Activity ◽  
Hiv Type 1 ◽  
Stimulation Of
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