Lateral Diffusion of Ibuprofen in Human Skin during Permeation Studies

2004 ◽  
Vol 17 (2) ◽  
pp. 84-90 ◽  
Author(s):  
G. Schicksnus ◽  
C.C. Müller-Goymann
Keyword(s):  
Human Skin ◽  
Lateral Diffusion ◽  
Permeation Studies

scholarly journals Penetration of Chlorhexidine into Human Skin

2008 ◽  
Vol 52 (10) ◽  
pp. 3633-3636 ◽  
Author(s):  
T. J. Karpanen ◽  
T. Worthington ◽  
B. R. Conway ◽  
A. C. Hilton ◽  
T. S. J. Elliott ◽  
...  
Keyword(s):  
Human Skin ◽  
High Performance ◽  
Skin Permeation ◽  
Full Thickness ◽  
Alternative Strategies ◽  
Thickness Skin ◽  
Skin Antisepsis ◽  
Permeation Studies ◽  
Franz Diffusion Cells

ABSTRACT This study evaluated a model of skin permeation to determine the depth of delivery of chlorhexidine into full-thickness excised human skin following topical application of 2% (wt/vol) aqueous chlorhexidine digluconate. Skin permeation studies were performed on full-thickness human skin using Franz diffusion cells with exposure to chlorhexidine for 2 min, 30 min, and 24 h. The concentration of chlorhexidine extracted from skin sections was determined to a depth of 1,500 μm following serial sectioning of the skin using a microtome and analysis by high-performance liquid chromatography. Poor penetration of chlorhexidine into skin following 2-min and 30-min exposures to chlorhexidine was observed (0.157 ± 0.047 and 0.077 ± 0.015 μg/mg tissue within the top 100 μm), and levels of chlorhexidine were minimal at deeper skin depths (less than 0.002 μg/mg tissue below 300 μm). After 24 h of exposure, there was more chlorhexidine within the upper 100-μm sections (7.88 ± 1.37 μg/mg tissue); however, the levels remained low (less than 1 μg/mg tissue) at depths below 300 μm. There was no detectable penetration through the full-thickness skin. The model presented in this study can be used to assess the permeation of antiseptic agents through various layers of skin in vitro. Aqueous chlorhexidine demonstrated poor permeation into the deeper layers of the skin, which may restrict the efficacy of skin antisepsis with this agent. This study lays the foundation for further research in adopting alternative strategies for enhanced skin antisepsis in clinical practice.

scholarly journals Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

2017 ◽  
Vol 18 (12) ◽  
pp. 2548 ◽  
Author(s):  
Marcelle Silva-Abreu ◽  
Lupe Espinoza ◽  
María Rodríguez-Lagunas ◽  
María-José Fábrega ◽  
Marta Espina ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Permeation ◽  
Pparγ Agonist ◽  
Inflammatory Processes ◽  
Permeation Studies

scholarly journals Alternatives to Biological Skin in Permeation Studies: Current Trends and Possibilities

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 152 ◽  
Author(s):  
Rabin Neupane ◽  
Sai H.S. Boddu ◽  
Jwala Renukuntla ◽  
R. Jayachandra Babu ◽  
Amit K. Tiwari
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
European Medicines Agency ◽  
Application Site ◽  
Artificial Membranes ◽  
Permeation Study ◽  
Ex Vivo Permeation ◽  
Permeation Studies ◽  
Animal Skin ◽  
Human Skin Models

The transdermal route of drugs has received increased attention in recent years due to numerous advantages over the oral and injectable routes, such as avoidance of the hepatic metabolism, protection of drugs from the gastrointestinal tract, sustained drug delivery, and good patient compliance. The assessment of ex vivo permeation during the pharmaceutical development process helps in understanding the product quality and performance of a transdermal delivery system. Generally, excised human skin relevant to the application site or animal skin is recommended for ex vivo permeation studies. However, the limited availability of the human skin and ethical issues surrounding the use of animal skin rendered these models less attractive in the permeation study. In the last three decades, enormous efforts have been put into developing artificial membranes and 3D cultured human skin models as surrogates to the human skin. This manuscript provides an insight on the European Medicines Agency (EMA) guidelines for permeation studies and the parameters affected when using Franz diffusion cells in the permeation study. The need and possibilities for skin alternatives, such as artificially cultured human skin models, parallel artificial membrane permeability assays (PAMPA), and artificial membranes for penetration and permeation studies, are comprehensively discussed.

scholarly journals Comparative Permeation Studies between Scale Region of Shed Snake Skin and Human Skin In vitro

2008 ◽  
Vol 3 (1) ◽  
pp. 444-450 ◽  
Author(s):  
Aroonsri Priprem ◽  
Chidchanok Khamlert ◽  
Thaned Pongjanyak ◽  
Sarayut Radapong ◽  
Theera Rittirod ◽  
...  
Keyword(s):  
Human Skin ◽  
Snake Skin ◽  
Permeation Studies

Qualitative and quantitative analysis of lateral diffusion of drugs in human skin

2018 ◽  
Vol 544 (1) ◽  
pp. 62-74 ◽  
Author(s):  
Hiep X. Nguyen ◽  
Ashana Puri ◽  
Sonalika Arup Bhattaccharjee ◽  
Ajay K. Banga
Keyword(s):  
Quantitative Analysis ◽  
Human Skin ◽  
Lateral Diffusion ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative
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