Interleukin-10 Suppresses Proliferation and Remodeling of Extracellular Matrix of Cultured Human Skin Fibroblasts

2004 ◽  
Vol 36 (1) ◽  
pp. 39-44 ◽  
Author(s):  
A. Moroguchi ◽  
K. Ishimura ◽  
K. Okano ◽  
H. Wakabayashi ◽  
T. Maeba ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Human Skin ◽  
Interleukin 10 ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Remodeling Of Extracellular Matrix

Apremilast interferes with the TGFβ1-induced transition of human skin fibroblasts into profibrotic myofibroblasts: in vitro study

Rheumatology ◽  
2020 ◽  
Vol 59 (12) ◽  
pp. 3927-3938
Author(s):  
Maurizio Cutolo ◽  
Stefano Soldano ◽  
Paola Montagna ◽  
Giulia Martinelli ◽  
Samuele Tardito ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Protein Synthesis ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Intracellular Signalling ◽  
Pde4 Inhibitor ◽  
Signalling Pathways ◽  
Human Skin Fibroblasts ◽  
Intracellular Signalling Pathways

Abstract Objectives Fibroblast-to-myofibroblast transition and extracellular matrix overproduction represent progressive events in chronic inflammatory and fibrotic diseases, in which TGFβ1 is one of the key mediators. Phosphodiesterase 4 (PDE4) acts as a proinflammatory enzyme through the degradation of cyclic adenosine monophosphate and it is overexpressed in skin fibroblasts. The study investigated how apremilast (a PDE4 inhibitor) interferes with the intracellular signalling pathways responsible for the TGFβ1-induced fibroblast-to-myofibroblast transition and profibrotic extracellular matrix protein synthesis. Methods Cultured human skin fibroblasts were stimulated with TGFβ1 (10 ng/ml) alone or combined with apremilast (1 and 10 μM) for 4, 16 and 24 h. Other aliquots of the same cells were previously stimulated with TGFβ1 and then treated with apremilast (1 and 10 μM) for 4, 16 and 24 h, always under stimulation with TGFβ1. Gene and protein expression of αSMA, type I collagen (COL1) and fibronectin were evaluated, together with the activation of small mothers against decapentaplegic 2 and 3 (Smad2/3) and extracellular signal-regulated kinase (Erk1/2) proteins. Results Apremilast reduced the TGFβ1-induced increase in αSMA, COL1 and fibronectin gene expression at 4 and 16 h, and protein synthesis at 24 h of treatment in cultured fibroblasts, even for cells already differentiated into myofibroblasts by way of a previous stimulation with TGFβ1. Apremilast inhibited the TGFβ1-induced Smad2/3 and Erk1/2 phosphorylation at 15 and 30 min. Conclusion Apremilast seems to inhibit in vitro the fibroblast-to-myofibroblast transition and the profibrotic activity induced by TGFβ1 in cultured human skin fibroblasts by downregulating Smad2/3 and Erk1/2 intracellular signalling pathways.

Hyaluronan knockdown extracellular matrix of cultured human skin fibroblasts by use of 4-methylumbelliferone

2001 ◽  
Vol 1223 ◽  
pp. 265-268
Author(s):  
Yasufumi Endo ◽  
Masaru Funahashi ◽  
Ikuko Kakizaki ◽  
Keiichi Takagaki ◽  
Masahiko Endo ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Shell Extracts from the Marine Bivalve Pecten maximus Regulate the Synthesis of Extracellular Matrix in Primary Cultured Human Skin Fibroblasts

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99931 ◽  
Author(s):  
Thomas Latire ◽  
Florence Legendre ◽  
Nicolas Bigot ◽  
Ludovic Carduner ◽  
Sabrina Kellouche ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Marine Bivalve ◽  
Pecten Maximus ◽  
Human Skin Fibroblasts

Long term UVA exposure inhibits extracellular matrix formation of human skin fibroblasts

1998 ◽  
Vol 16 ◽  
pp. S227
Author(s):  
Munehiro Iketani ◽  
Yukiko Kurihara ◽  
Toshio Nishiyama ◽  
Yasuhisa Nakayama
Keyword(s):  
Extracellular Matrix ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts
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