Molecular characterization of three gonad cell lines

2003 ◽  
Vol 101 (3-4) ◽  
pp. 242-249 ◽  
Author(s):  
A. Beverdam ◽  
D. Wilhelm ◽  
P. Koopman
Keyword(s):  
Molecular Characterization ◽  
Cell Lines ◽  
Gonad Cell

Abstract A45: In depth molecular characterization of novel PI3K-mTOR inhibitor resistant NSCLC cell lines

2015 ◽  
Author(s):  
Susan Heavey ◽  
Paul Dowling ◽  
Sinéad Toomey ◽  
Aoife Carr ◽  
Bryan Hennessy ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cell Lines ◽  
Mtor Inhibitor ◽  
Nsclc Cell

scholarly journals Molecular characterization of conditionally immortalized cell lines derived from mouse early embryonic inner ear

2004 ◽  
Vol 231 (4) ◽  
pp. 815-827 ◽  
Author(s):  
John A. Germiller ◽  
Elizabeth C. Smiley ◽  
Amanda D. Ellis ◽  
Jessica S. Hoff ◽  
Ian Deshmukh ◽  
...  
Keyword(s):  
Inner Ear ◽  
Molecular Characterization ◽  
Cell Lines ◽  
Immortalized Cell Lines ◽  
Immortalized Cell

Phenotypic and molecular characterization of inducible human neuroblastoma cell lines

1988 ◽  
Vol 39 (3) ◽  
pp. 216-227 ◽  
Author(s):  
Herman Yeger ◽  
Reuben Baumal ◽  
Gladys Pawlin ◽  
Patricia Tonin ◽  
Lynette Nissen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Neuroblastoma Cell Lines

Biological and Molecular Characterization by Integrative Genomics Approach of CMA-03/06, a Newly Established Interleukin-6 Independent Variant of the CMA-03 Human Myeloma Cell Line.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1814-1814
Author(s):  
Donata Verdelli ◽  
Lucia Nobili ◽  
Katia Todoerti ◽  
Laura Mosca ◽  
Sonia Fabris ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Molecular Characterization ◽  
Cell Lines ◽  
Stromal Cells ◽  
Interleukin 6 ◽  
Myeloma Cell ◽  
Growth And Survival ◽  
Genome Wide

Abstract Abstract 1814 Poster Board I-840 Background The growth and survival of multiple myeloma (MM) cells in the bone marrow microenvironment is regulated by functional complex interactions between the tumor cells and the surrounding bone marrow stromal cells mediated by adhesion molecules and the production of several cytokines of which interleukin-6 (IL-6) has been identified as the most important. Major advances in the investigation of MM biology were made possible by the availability of human myeloma cell lines (HMCLs). The IL-6-dependent CMA-03 cell line was established in our laboratory from a peritoneal effusion of a refractory relapsed MM patient. By gradually decreasing the IL-6 added to the culture, an IL-6-independent variant, CMA-03/06, could be obtained. Aims. To perform a biological and molecular characterization of this novel cell line, and to provide insights into the signaling pathways and target genes involved in the growth and survival of CMA-03/06. Methods. The growth, immunophenotypic, cytogenetic and fluorescence in situ hybridization (FISH) characterization of CMA-03/06 cell line was performed by means of standard procedures. IL-6 production into the culture media was determined using a high sensitivity IL-6 specific ELISA. Genome-wide profiling data were generated by means of Affymetrix GeneChip® Human Mapping 250K Nsp arrays; copy number (CN) alterations were calculated using the DNAcopy Bioconductor package, based on circular binary segmentation method. Global gene expression profiling (GEP) was performed by means of the GeneChip® Human Gene 1.0 ST Arrays (Affymetrix); the supervised analyses were done using the SAM software version 3.0. Results Unlike CMA-03, the addition of IL-6 to the culture medium of CMA-03/06 cells or co-culture with multipotent mesenchymal stromal cells did not induce an increase in CMA-03/06 proliferation. IL-6 was not detected in the supernatants from either CMA-03 or CMA-03/06 cell lines within 48 h, suggesting that the IL-6 independence of CMA03/06 cells is not a result of the development of an autocrine IL-6 loop. Nevertheless, IL-6 induced the activation of STAT3 and STAT1 in both cell lines, even if a slight constitutive STAT3 phosphorylation was found in CMA-03/06. The immunophenotypic analysis showed a significant difference in the expression of three antigens in the 2 cell lines: CD45 was considerably reduced in CMA-03/06 cells, whereas they were found positive for both chains of IL-6 receptor, CD126 and CD130, almost undetectable in CMA-03. Conventional cytogenetic and FISH analyses did not reveal differences between the 2 HMCLs. The genome-wide analysis allowed the identification of about 100 altered chromosomal regions common to both HMCLs, mostly DNA gains. Comparison of CMA-03/06 and CMA-03 cells evidenced a different CN in only 15 small chromosomal regions, 8 of which did not contain any transcript, whereas few genes were located on the other ones. GEP analysis of CMA-03/06 compared with CMA-03 identified 21 upregulated and 47 downregulated genes, many of which particularly relevant for MM biology, mainly involved in cellular signaling, cell cycle, cell adhesion, cell development, regulation of transcription, immunologic, inflammatory or defense activity, apoptosis. None of the genes differentially expressed in CMA-03/06 compared with CMA-03 except 1 were positioned on the chromosomal regions showing a different CN. Finally, CMA-03/06 cell line showed a lower susceptibility to camptothecin-induced apoptosis compared to CMA-03 cells. Conclusions Our data show the IL-6 independence of CMA-03/06 cell line in the absence of an autocrine IL-6 loop; the cells, however, maintain the IL-6 signaling pathway responsiveness. A consistent number of genes particularly relevant for MM biology were found deregulated in CMA-03/06 cell line compared with CMA-03. Furthermore, CMA-03/06 cell line shows an increased resistance to apoptosis. The novel CMA03/06 cell line may thus represent a suitable model for studies investigating molecular mechanisms involved in clonal evolution towards IL-6 and/or stroma-independent growth and survival of myeloma cells. Disclosures No relevant conflicts of interest to declare.

scholarly journals Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes

2015 ◽  
Vol 131 ◽  
pp. 42-55 ◽  
Author(s):  
Anne M. Terrell ◽  
Deepti Anand ◽  
Sylvie F. Smith ◽  
Christine A. Dang ◽  
Stephanie M. Waters ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Molecular Characterization ◽  
Cell Lines ◽  
Lens Epithelial Cell ◽  
Rna Granules ◽  
Epithelial Cell Lines ◽  
Mouse Lens

Pharmacological and molecular characterization of intrinsic and acquired doxorubicin resistance in murine tumor cell lines

1993 ◽  
Vol 119 (9) ◽  
pp. 527-532 ◽  
Author(s):  
Brigitte Schott ◽  
Danielle Londos-Gagliardi ◽  
Colette Ries ◽  
Sylvie Huet ◽  
Jacques Robert
Keyword(s):  
Tumor Cell ◽  
Molecular Characterization ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Doxorubicin Resistance ◽  
Murine Tumor
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