Regulation of Follicle-Stimulating Hormone Secretion by the Interactions of Activin-A, Dexamethasone and Testosterone in Anterior Pituitary Cell Cultures of Male Rats

2003 ◽  
Vol 77 (5) ◽  
pp. 298-304 ◽  
Author(s):  
Angela M.O. Leal ◽  
Amy L. Blount ◽  
Cynthia J. Donaldson ◽  
Louise M. Bilezikjian ◽  
Wylie W. Vale
Keyword(s):  
Cell Cultures ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Hormone Secretion ◽  
Activin A ◽  
Pituitary Cell ◽  
Male Rats ◽  
Anterior Pituitary Cell ◽  
Stimulating Hormone

Des-acetylated variants of α-melanocyte-stimulating hormone and β-endorphin can antagonize the mammotrope-recruiting activity of their acetylated forms

1993 ◽  
Vol 139 (2) ◽  
pp. 295-300 ◽  
Author(s):  
E. Ellerkmann ◽  
R. D. Kineman ◽  
T. E. Porter ◽  
L. S. Frawley
Keyword(s):  
Cell Cultures ◽  
Anterior Pituitary ◽  
Relative Number ◽  
Pituitary Cell ◽  
Ovariectomized Rats ◽  
Anterior Pituitary Cell ◽  
Melanocyte Stimulating Hormone ◽  
Absolute Requirement ◽  
Stimulating Hormone

ABSTRACT We have previously reported that hypophysial neurointermediate lobe peptides, di-acetylated α-melanocyte-stimulating hormone (di-ac-α-MSH) and N-acetylated β-endorphin (N-ac-β-END), can acutely increase the relative number of prolactin-secreting cells in anterior pituitary cell cultures from ovariectomized rats. Inasmuch as the des-acetylated forms of these peptides (des-ac-α-MSH and β-END) were not effective in this regard, we concluded that acetylation was an absolute requirement for manifestation of the recruitment response. The aim of the present study was to determine whether these des-acetylated variants could antagonize the mammotrope-recruiting activity of their acetylated congeners. Treatment of anterior pituitary cell cultures with di-ac-α-MSH and N-ac-β-END increased the relative amount of prolactin secretors above control values. Interestingly, des-acetylated variants of α-MSH and β-END blocked the mammotrope-recruitment activity of their respective acetylated forms. In addition, β-END antagonized the mammotrope-recruitment activity of di-ac-α-MSH while des-ac-α-MSH did not attenuate the stimulatory effect of N-ac-β-END. Given that mammotropes maintained in vivo are exposed to all these peptides, it is possible that these acetylated and non-acetylated congeners may act in an opposing manner to regulate dynamic prolactin release. Journal of Endocrinology (1993) 139, 295–300

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