New Gastric Epithelial Cell Lines from Mice Transgenic for Temperature-Sensitive Simian Virus 40 Large T Antigen Show Distinct Types of Cell Differentiation

Digestion ◽  
2003 ◽  
Vol 67 (1-2) ◽  
pp. 71-81 ◽  
Author(s):  
Yoshiaki Tabuchi ◽  
Yuko Arai ◽  
Hiroki Shioya ◽  
Ryosuke Kuribayashi ◽  
Kotaro Ishibashi ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Gastric Epithelial Cell ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Epithelial Cell Lines

scholarly journals Cell lines established by a temperature-sensitive simian virus 40 large-T-antigen gene are growth restricted at the nonpermissive temperature.

1989 ◽  
Vol 9 (4) ◽  
pp. 1672-1681 ◽  
Author(s):  
P S Jat ◽  
P A Sharp
Keyword(s):  
Cell Lines ◽  
Growth Arrest ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Nonpermissive Temperature ◽  
Large T Antigen ◽  
Clonal Cell Lines

The thermolabile large T antigen, encoded by the simian virus 40 early-region mutant tsA58, was used to establish clonal cell lines derived from rat embryo fibroblasts. These cell lines grew continuously at the permissive temperature but upon shift-up to the nonpermissive temperature showed rapidly arrested growth. The growth arrest occurred in either the G1 or G2 phase of the cell cycle. After growth arrest, the cells remained metabolically active as assayed by general protein synthesis and the ability to exclude trypan blue. The inability of these cell lines to divide at the nonpermissive temperature was not readily complemented by the exogenous introduction of other nuclear oncogenes. This finding suggests that either these genes establish cells via different pathways or that immortalization by one oncogene results in a finely balanced cellular state which cannot be adequately complemented by another establishment gene.

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