No Evidence of IFN-γ Increase in the Serum of HIV-Positive and Healthy Subjects after Subcutaneous Injection of a Non-Fermented Viscum album L. Extract

1998 ◽  
Vol 16 (4) ◽  
pp. 157-164 ◽  
Author(s):  
Matthias Stoss ◽  
Robert W. Gorter
Keyword(s):  
Subcutaneous Injection ◽  
Healthy Subjects ◽  
Viscum Album ◽  
Hiv Positive ◽  
Ifn Γ

scholarly journals Th17/Treg-Related Transcriptional Factor Expression and Cytokine Profile in Patients With Rheumatoid Arthritis

2020 ◽  
Vol 11 ◽  
Author(s):  
Agnieszka Paradowska-Gorycka ◽  
Anna Wajda ◽  
Katarzyna Romanowska-Próchnicka ◽  
Ewa Walczuk ◽  
Ewa Kuca-Warnawin ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Healthy Subjects ◽  
Treg Cells ◽  
Cytokine Profile ◽  
Diagnostic Biomarkers ◽  
Diagnostic Potential ◽  
Potential Biomarker ◽  
Cytokine Levels ◽  
Ifn Γ ◽  
Very High

ObjectivesThe aim of our study was to determine whether there is a correlation between transcription factors expression and Th17/Treg ratio, cytokine profile in the RA phenotype as well as to identify transcription factors that could be a potential biomarker for RA.MethodsThe study was conducted on 45 patients with RA, 27 patients with OA and 46 healthy controls (HCs). Th17 and Treg frequency was determined by flow cytometry (15 patients with RA/OA and 15 subjects of HC). Gene expression was estimated by qPCR, and the serum cytokine levels were determined by ELISA.ResultsThe percentage of Treg (CD4+CD25highCD127-) cells in RA patients was lower than in OA patients or HCs. Proportions of Th17 (CD4+CCR6+CXCR3-) cells were higher in RA and OA in comparison to HCs. STAT5 showed a very high expression in the blood of RA patients compared to healthy subjects. The expression of STAT5 and HELIOS was not detected in Th17 cells. A positive correlation between SMAD3 and STAT3 in RA patients was observed. Negative correlations between HIF-1A and SMAD2 in RA Treg cells and DAS-28 score were observed. The range of serum of IL-17 and IL-21 were higher in RA patients than in OA patients. Concentrations of serum IL-2 and IFN-γ were higher in RA and OA patients than in healthy subjects. Based on the ROC analysis, the diagnostic potential of the combination of HIF1A, SMAD3 and STAT3, was determined at AUC 0.95 for distinguishing RA patients from HCs. For distinguishing RA patients from OA patients the diagnostic potential of the combination of SMAD2, SMAD3, SMAD4 and STAT3, was determined at AUC 0.95.ConclusionBased on our study, we conclude that SMAD3 and STAT3 could be potential diagnostic biomarkers for RA.

scholarly journals Heparin-Binding Hemagglutinin Induces IFN-γ+IL-2+IL-17+Multifunctional CD4+T Cells during Latent but Not Active Tuberculosis Disease

2012 ◽  
Vol 19 (5) ◽  
pp. 746-751 ◽  
Author(s):  
André G. Loxton ◽  
Gillian F. Black ◽  
Kim Stanley ◽  
Gerhard Walzl
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Culture Supernatant ◽  
Interleukin 2 ◽  
Latent Tuberculosis ◽  
Hiv Positive ◽  
Heparin Binding ◽  
Household Contacts ◽  
Ifn Γ ◽  
Tuberculosis Disease

ABSTRACTThe mycobacterial heparin-binding hemagglutinin (HBHA) protein induces a potent gamma interferon (IFN-γ) response in latent tuberculosis (TB) infection and is a candidate vaccine and diagnostic antigen. We have assessed HBHA-specific intracellular IFN-γ, interleukin-2 (IL-2), and IL-17 production by CD4+T cells in TB cases and household contacts (HHCs) as well as the level of secreted IFN-γ in whole-blood culture supernatant. HHCs were further classified as tuberculin skin test (TST) positive or negative, and the group was also divided as HIV positive or negative. Our study revealed that HBHA induces multifunctional IFN-γ-, IL-2-, and IL-17-coexpressing CD4+T cells in HHCs but not in active TB cases; however, IFN-γ levels in culture supernatant did not differ between participant groups. Further studies are needed to completely understand how HBHA induces immune responses in different disease groups.

FTY720 (fingolimod) treatment tips the balance towards less immunogenic antigen-presenting cells in patients with multiple sclerosis

2015 ◽  
Vol 21 (14) ◽  
pp. 1811-1822 ◽  
Author(s):  
Felix Luessi ◽  
Stefan Kraus ◽  
Bettina Trinschek ◽  
Steffen Lerch ◽  
Robert Ploen ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Dendritic Cells ◽  
Healthy Subjects ◽  
Ex Vivo ◽  
Antigen Presenting Cells ◽  
Direct Effects ◽  
Quantitative Analyses ◽  
Ifn Γ ◽  
Multiple Sclerosis Patients ◽  
Antigen Presenting

Objective: We aimed to clarify whether fingolimod has direct effects on antigen-presenting cells in multiple sclerosis patients. Methods: Frequency and phenotype of directly ex vivo dendritic cells and monocytes were analyzed in 43 individuals, including fingolimod-treated and untreated multiple sclerosis patients as well as healthy subjects. These cells were further stimulated with lipopolysaccharide to determine functional effects of fingolimod treatment. Results: Absolute numbers of CD1c+ dendritic cells and monocytes were not significantly reduced in fingolimod-treated patients indicating that fingolimod did not block the migration of antigen-presenting cells to peripheral blood. CD86 was upregulated on CD1c+ dendritic cells and thus their activation was not impaired under fingolimod treatment. Quantitative analyses of gene transcription in cells and protein content in supernatants from ex vivo CD1c+ dendritic cells and monocytes, however, showed lower secretion of TNFα, IL1-β and IL-6 upon lipopolysaccharide-stimulation. These results could be matched with CD4+MOG-specific transgenic T cells exhibiting reduced levels of TNFα and IFN-γ but not IL-4 upon stimulation with murine dendritic cells loaded with MOG, when treated with fingolimod. Conclusions: Our data indicate that fingolimod – apart from trapping lymphocytes in lymph nodes – exerts its disease-modulating activity by rebalancing the immune tolerance networks by modulation of antigen-presenting cells.

scholarly journals INTERFERON GAMMA EXPRESSION CD8+-T LYMPHOCYTE WITH ESAT-6-CFP-10 FUSION ANTIGEN STIMULATION BETWEEN ACTIVE TUBERCULOSIS, LATENT TUBERCULOSIS AND HEALTHY PEOPLE

2018 ◽  
Vol 24 (3) ◽  
Author(s):  
Holland Lydia Marpaung ◽  
Betty Agustina ◽  
Jusak Nugraha ◽  
Fransiska Fransiska
Keyword(s):  
Interferon Gamma ◽  
T Lymphocyte ◽  
Healthy Subjects ◽  
Vaccine Candidate ◽  
Healthy People ◽  
Antigen Stimulation ◽  
Cd8 T Lymphocyte ◽  
Latent Tb ◽  
Ifn Γ ◽  
Fusion Antigen

Tuberculosis (TB) is an infectious disease in the world causing a global problem. Vaccination with Purified Protein Derivative (PPD) still cannot prevent tuberculosis in Indonesia. Interferon-gamma (IFN-γ) produced by CD8+-T lymphocyte has an important role in eliminating Mycobacterium tuberculosis. The vaccine candidate antigenic test was done to observe the inducible ability of IFN-γ as a main protection cytokine. This study aim was to research the difference of IFN-γ expression CD8+-T lymphocyte percentage with ESAT-6-CFP-10 fusion antigen stimulations as a TB vaccine candidate. This research is a quasi-experimental study design in the laboratory by ESAT-6-CFP-10 fusion antigen-stimulated Peripheral Blood Mononuclear Cells (PBMC) culture in vitro in TB patients, latent TB, and healthy subjects’ groups. IFN-γ expression CD8+-T lymphocyte percentage were examined by flow cytometry BDFACSCalibur with results: without antigen fusion stimulation IFN-γ expression CD8+-T lymphocyte percentage mean (TB patients 2,560, latent TB 2,173, and healthy people 2,153) and with antigen fusion (TB patients 3,039, latent TB 2,471, and healthy people 2,405). There was no significant difference in fusion antigen stimulation PBMC between TB patients, latent TB, and healthy subjects’ group, and also within groups.

scholarly journals Analysis of tear inflammatory molecules and clinical correlations in evaporative dry eye disease caused by meibomian gland dysfunction

2020 ◽  
Vol 40 (11) ◽  
pp. 3049-3058
Author(s):  
Xingdi Wu ◽  
Xiang Chen ◽  
Yajuan Ma ◽  
Xueqi Lin ◽  
Xuewen Yu ◽  
...  
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Healthy Subjects ◽  
Meibomian Gland ◽  
Meibomian Gland Dysfunction ◽  
Surface Parameters ◽  
Tnf Α ◽  
Evaporative Dry Eye ◽  
Ifn Γ ◽  
Inflammatory Molecules

Abstract Purpose To compare the levels of inflammatory molecules in tear samples between patients with meibomian gland dysfunction (MGD)-related evaporative dry eye (EDE) and healthy subjects and to analyze the correlations between the levels of tear inflammatory molecules and ocular surface parameters. Methods A total of 30 MGD-related EDE patients (48 eyes) and ten healthy volunteers (15 eyes) were enrolled. Dry eye-related examinations and questionnaires were obtained from all participants. The levels of nine inflammatory molecules were determined through multiplex bead analysis. Results Inflammatory molecules including ICAM-1, IFN-γ, CXCL8/IL-8, IL-6, TNF-α and IL-12p70 were detected in 100% of the patients, while IL-1α, IL-1β and IL-10 were detected in 56.25%, 13.60% and 45.83% of the patients, respectively. Moreover, ICAM-1, IL-8, IL-6, TNF-α, IL-12p70 and IFN-γ were detected in 86.67–100% of the healthy subjects, and the detection rates of IL-10, IL-1α and IL-1β were below 50%. The levels of IL-8, IL-6, IFN-γ and ICAM-1 were significantly higher in the patient group compared with the control group. In addition, IL-8 and IL-6 were negatively correlated with Schirmer I test. Besides, IFN-γ was negatively correlated with tear film breakup time. Furthermore, ICAM-1 and IL-6 were positively correlated with meibography score. Conclusions Collectively, patients with MGD-related EDE had higher levels of inflammatory molecules in their tears, and some molecules were correlated with ocular surface parameters. These findings suggested that inflammation played an important role in MGD-related EDE, and several inflammatory molecules could be used in the diagnosis and the treatment of MGD-related EDE.

Cytokine Production Profile of CD4+ T Cells From Patients with Immune Thrombocytopenia.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4460-4460
Author(s):  
Shanhua Zou ◽  
Feng Li ◽  
Weiguang Wang ◽  
Yunfeng Cheng
Keyword(s):  
T Cells ◽  
Real Time ◽  
Real Time Pcr ◽  
Cd4 T Cells ◽  
Healthy Subjects ◽  
Immune Thrombocytopenia ◽  
Plasma Concentrations ◽  
Platelet Destruction ◽  
Expression Levels ◽  
Ifn Γ

Abstract Abstract 4460 Introduction To balance self-tolerance and immunity, the immune system depends on both activation mechanisms and down regulatory mechanisms. CD4+ T cells are important for the regulation of immune responses and they exert their effects through the secretion of cytokines. Immune thrombocytopenia(ITP) is a common hematologic disorder manifested by immune-mediated platelet destruction. ITP in adults often has a persistent course and frequently requires treatment to prevent bleeding. The mechanism of ITP has historically been attributed to platelet autoantibody production and the resultant platelet destruction. However, more recent evidence suggests that cell immunity pathogenesis plays an important role in ITP. Methods Real-time PCR assays was used for the quantification of mRNA of cytokines IL-2, IL-4, IL-6, IL-10, IL-12, IL-18, IL-23, IL-17, IFN-γ, TNF-α and TGF-β in CD4+ T cells from adult ITP patients and healthy subjects. The mRNA of T-bet, GATA-3, RORγt, and FOXP3 were also analyzed using Real-time PCR. The plasma concentrations of IL-2, IL-4, IL-6, IL-10, IL-12, IL-18, IL-23, IFN-γ, TNF and IL-17 were determined by enzyme-linked immunosorbent assay at the same time. Results The plasma concentrations of IL-2, IL-17 and IFN-γ in CD4+T cells were increased, while levels of IL-4, IL-6, IL-10 and TGF-β were decreased, comparing adult patients with ITP with healthy subjects. Patients with ITP presented significant increased mRNA expression levels on IL-2,IL-17,T-bet and RORγt. Conclusions In adult ITP patients, the expression levels of IL-2 and IL-17 were up-regulated, expression levels of TGF-β and IL-10 were down-regulated, suggesting that ITP is a Th1 and Th17 predominant disease although the precise mechanisms await further study. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Flavonoid Mixture Inhibits Mycobacterium tuberculosis Survival and Infectivity

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 851 ◽  
Author(s):  
Ruoqiong Cao ◽  
Garrett Teskey ◽  
Hicret Islamoglu ◽  
Myra Gutierrez ◽  
Oscar Salaiz ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Healthy Subjects ◽  
Granuloma Formation ◽  
Pathogenic Potential ◽  
Flavonoid Intake ◽  
Favorable Influence ◽  
Ifn Γ ◽  
Healthy Participants

Background: Flavonoids have been shown to exert anti-pathogenic potential, but few studies have investigated their effects on Mycobacterium tuberculosis (Mtb) infectivity. We hypothesized that a flavonoid mixture would have a favorable influence on cell death and the resolution of Mtb infection in THP-1 macrophages and in granulomas derived from both healthy participants and those with type 2 diabetes mellitus (T2DM). METHODS: THP-1 macrophages, and in vitro granulomas from healthy participants (N = 8) and individuals with T2DM (N = 5) were infected with Mtb. A mixed flavonoid supplement (MFS) at a concentration of 0.69 mg per ml was added as treatment to Mtb infected THP-1 macrophages and granulomas for 8 to 15 days. RESULTS: MFS treatment significantly reduced the intracellular Mtb survival, increased cell density, aggregation, and granuloma formation, and increased glutathione (GSH) levels. IL-12 and IFN-γ levels tended to be higher and IL-10 lower when Mtb infected THP-1 macrophages and granulomas obtained from healthy subjects were treated with MFS compared to control. CONCLUSIONS: MFS treatment exerted a strong influence against Mtb infectivity in THP-1 macrophages and in granulomas including antimycobacterial effects, GSH enrichment, cytokine regulation, and augmented granuloma formation. Our data support the strategy of increased flavonoid intake for managing tuberculosis.

scholarly journals SOLUBLE ST2 AND CD163 AS POTENTIALBIOMARKERS TO DIFFERENTIATE PRIMARY HEMOPHAGOCYTIC LYMPHOHISTIOCYTOSIS FROM MACROPHAGE ACTIVATION SYNDROME

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhuo Gao ◽  
Yini Wang ◽  
Jingshi Wang ◽  
Jia Zhang ◽  
Zhao Wang
Keyword(s):  
Healthy Subjects ◽  
Macrophage Activation Syndrome ◽  
Macrophage Activation ◽  
Serum Levels ◽  
Haemophagocytic Lymphohistiocytosis ◽  
Soluble St2 ◽  
Tnf Α ◽  
Significant Difference ◽  
Ifn Γ ◽  
Activation Syndrome

Abstract Background and Objective: The differentiation of primary haemophagocytic lymphohistiocytosis (pHLH) and macrophage activation syndrome (MAS) poses a challenge to hematologists. The aim of this study was (1) to compare the levels of soluble ST2 (sST2), sCD163, IL-10, IFN-γ, TNF-α and IL-18 in patients with pHLH and MAS and (2) to investigate whether they can help differentiate the two diseases. Methods: A total of 54 participants were recruited in this study, including 12 pHLH patients, 22 MAS patients and 20 healthy subjects. We measured the levels of sST2 and sCD163 in serum by ELISA. The serum levels of IL-10, IFN-γ, TNF-α and IL-18 were detected using a Luminex 200 instrument. Results: The serum levels of sST2 and sCD163 in MAS patients were markedly higher than that in pHLH patients (363.13 ± 307.24 ng/ml vs 80.75 ± 87.04 ng/ml, P = 0.004; 3532.72 ± 2479.68 ng/ml vs 1731.96 ± 1262.07 ng/ml, P = 0.046). There was no significant difference in the expression of IFN-γ (306.89 ± 281.60 pg/ml vs 562.43 ± 399.86 pg/ml), IL-10 (20.40 ± 30.49 pg/ml vs 8.3 ± 13.14 pg/ml), IL-18 (463.33 ± 597.04 pg/ml vs 1247.82 ± 1318.58 pg/ml) and TNF-α (61.48 ± 84.69 pg/ml vs 106.10 ±77.21 pg/ml) between pHLH and MAS. Conclusion: Patients with pHLH and MAS show some differences in cytokine profiles. The elevated levels of IFN-γ, IL-10, IL-18 and TNF-α can contribute to the diagnosis of HLH, but may not discriminate pHLH from MAS. Levels of sST2 and sCD163 may serve as markers to distinguish pHLH from MAS.

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