Expansion and Proliferation of Skin-Homing T Cells in Atopic Dermatitis as Assessed at the Single Cell Level

2003 ◽  
Vol 130 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Thomas Jung ◽  
Stephanie Schulz ◽  
Karolin Zachmann ◽  
Christine Neumann
Keyword(s):  
T Cells ◽  
Atopic Dermatitis ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Skin Homing

Detection of intracellular expression and secretion of interferon-γ at the single-cell level after activation of human T cells with tetanus toxoidin vitro

1990 ◽  
Vol 20 (5) ◽  
pp. 1085-1089 ◽  
Author(s):  
Lalitha Kabilan ◽  
Gudrun Andersson ◽  
Francesco Lolli ◽  
Hans-peter Ekre ◽  
Tomas Olsson ◽  
...  
Keyword(s):  
T Cells ◽  
Single Cell ◽  
Interferon Γ ◽  
Single Cell Level ◽  
Cell Level ◽  
Human T Cells ◽  
Intracellular Expression

Enhanced IFN-γ Production in Vitro by CD8+ T Cells in Hemophiliacs with AIDS as Demonstrated on the Single-Cell Level

1999 ◽  
Vol 92 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Y. Pae ◽  
H. Minagawa ◽  
J. Hayashi ◽  
S. Kashiwagi ◽  
Y. Yanagi
Keyword(s):  
T Cells ◽  
Single Cell ◽  
Cd8 T Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Ifn Γ

scholarly journals Single-cell RNA-Seq reveals the link between CD45 isoforms and tumor-infiltrating T cells heterogeneity in liver cancer

2020 ◽  
Author(s):  
Biaofeng Zhou ◽  
Shang Liu ◽  
Liang Wu ◽  
Yan Sun ◽  
Jie Chen ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Single Cell ◽  
Cell Function ◽  
Effector Memory ◽  
Single Cell Level ◽  
Cell Level ◽  
Cd45 Isoforms ◽  
And Function

AbstractCD45 isoforms play a major role in characterizing T cell function, phenotype, and development. However, there is lacking comprehensive interrogation about the relationship between CD45 isoforms and T lymphocytes from cancer patients at the single-cell level yet. Here, we investigated the CD45 isoforms component of published 5,063 T cells of hepatocellular carcinoma (HCC), which has been assigned functional states. We found that the distribution of CD45 isoforms in T lymphocytes cells depended on tissue resource, cell type, and functional state. Further, we demonstrated that CD45RO and CD45RA dominate in characterizing the phenotype and function of T cell though multiple CD45 isoforms coexist in T cells, through a novel alternative splicing pattern analysis. We identified a novel development trajectory of tumor-infiltrating T cells from Tcm to Temra (effector memory T cells re-expresses CD45RA) after detecting two subpopulations in state of transition, Tcm (central memory T) and Tem (effector memory T). Temra, capable of high cytotoxic characteristics, was discovered to be associated with the stage of HCC and may be a target of immunotherapy. Our study presents a comprehension of the connection between CD45 isoforms and the function, states, sources of T lymphocytes cells in HCC patients at the single-cell level, providing novel insight for the effect of CD45 isoforms on T cell heterogeneity.

scholarly journals Quantifying the efficacy of checkpoint inhibitors on CD8+ cytotoxic T cells for immunotherapeutic applications via single-cell interaction

2020 ◽  
Vol 11 (11) ◽  
Author(s):  
Matthew Ryan Sullivan ◽  
Giovanni Stefano Ugolini ◽  
Saheli Sarkar ◽  
Wenjing Kang ◽  
Evan Carlton Smith ◽  
...  
Keyword(s):  
T Cells ◽  
Combination Therapy ◽  
Single Cell ◽  
Checkpoint Inhibitors ◽  
Cell Killing ◽  
Calcium Flux ◽  
Single Cell Level ◽  
Cellular Interactions ◽  
Cell Level ◽  
The Impact

AbstractThe inhibition of the PD1/PDL1 pathway has led to remarkable clinical success for cancer treatment in some patients. Many, however, exhibit little to no response to this treatment. To increase the efficacy of PD1 inhibition, additional checkpoint inhibitors are being explored as combination therapy options. TSR-042 and TSR-033 are novel antibodies for the inhibition of the PD1 and LAG3 pathways, respectively, and are intended for combination therapy. Here, we explore the effect on cellular interactions of TSR-042 and TSR-033 alone and in combination at the single-cell level. Utilizing our droplet microfluidic platform, we use time-lapse microscopy to observe the effects of these antibodies on calcium flux in CD8+ T cells upon antigen presentation, as well as their effect on the cytotoxic potential of CD8+ T cells on human breast cancer cells. This platform allowed us to investigate the interactions between these treatments and their impacts on T-cell activity in greater detail than previously applied in vitro tests. The novel parameters we were able to observe included effects on the exact time to target cell killing, contact times, and potential for serial-killing by CD8+ T cells. We found that inhibition of LAG3 with TSR-033 resulted in a significant increase in calcium fluctuations of CD8+ T cells in contact with dendritic cells. We also found that the combination of TSR-042 and TSR-033 appears to synergistically increase tumor cell killing and the single-cell level. This study provides a novel single-cell-based assessment of the impact these checkpoint inhibitors have on cellular interactions with CD8+ T cells.

Hepatitis B vaccine-specific CD4+ T cells can be detected and characterised at the single cell level: Limited usefulness of dendritic cells as signal enhancers

2008 ◽  
Vol 330 (1-2) ◽  
pp. 1-11 ◽  
Author(s):  
Nicolle H.R. Litjens ◽  
Martin Huisman ◽  
Carla C. Baan ◽  
Corné J. van Druningen ◽  
Michiel G.H. Betjes
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Hepatitis B ◽  
Single Cell ◽  
Cd4 T Cells ◽  
Hepatitis B Vaccine ◽  
Single Cell Level ◽  
Cell Level ◽  
Limited Usefulness

Phagocytic dendritic cells from myelomas activate tumor-specific T cells at a single cell level

Blood ◽  
2001 ◽  
Vol 97 (9) ◽  
pp. 2808-2814 ◽  
Author(s):  
Zlatko Dembic ◽  
John-Arne Røttingen ◽  
Jérôme Dellacasagrande ◽  
Karl Schenck ◽  
Bjarne Bogen
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Single Cell ◽  
Mhc Class Ii ◽  
Messenger Rna ◽  
Magnetic Beads ◽  
Specific Antigen ◽  
Class Ii ◽  
Single Cell Level ◽  
Cell Level

Abstract Antigen-presenting cells (APCs) from subcutaneous mouse MOPC315 plasmacytoma phagocytosed immunoglobulin G–coated magnetic beads, enabling efficient isolation within 2 hours by magnetic separation (APC-MB). Cell morphology was heterogeneous, with some of the cells having dendrites. The surface phenotype of purified tumor APCs-MB was CD11b+, CD11c+, CD40+, CD80+, CD86+, and MHC class II+. Tumor APCs-MB expressed messenger RNA for fractalkine and ABCD-1 chemokines, and for CC-type chemokine receptors CCR5 and CCR7, indicating the presence of mature dendritic cells (DCs). Visualized at a single cell level within 4 hours after disruption of the tumor, APCs-MB induced rapid Ca++ mobilization in MHC class II–restricted tumor idiotype (Id)–specific cloned CD4+ T cells. In long-term assays, tumor APCs-MB induced proliferation of naive T cells from Id-specific T-cell receptor transgenic mice. The results suggest that tumor APCs-MB represent a heterogeneous cell population that includes myeloid-derived DCs of various stages of maturation. A considerable fraction (≥ 15%) of DCs is spontaneously primed with tumor-specific antigen.

Assessment of caspase mediated degradation of linker for activation of T cells (LAT) at a single cell level

2013 ◽  
Vol 389 (1-2) ◽  
pp. 9-17 ◽  
Author(s):  
Mikołaj Kłossowicz ◽  
Bożena Ścirka ◽  
Joanna Suchanek ◽  
Karolina Marek-Bukowiec ◽  
Pawel Kisielow ◽  
...  
Keyword(s):  
T Cells ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level
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