Reactivity of Secretory IgA Antibodies in Breast Milk from 107 Japanese Mothers to 20 Environmental Antigens

Neonatology ◽  
2002 ◽  
Vol 82 (4) ◽  
pp. 238-242 ◽  
Author(s):  
Takeshi Takahashi ◽  
Yumi Yoshida ◽  
Seiko Hatano ◽  
Yoshiko Sugita-Konishi ◽  
Shizunobu Igimi ◽  
...  
Keyword(s):  
Breast Milk ◽  
Secretory Iga ◽  
Iga Antibodies ◽  
Japanese Mothers ◽  
Environmental Antigens

Differences in Level and Avidity of Secretory IgA Antibodies in Breast Milk of Swedish, Indian and Japanese Mothers to Soybean Protein

1991 ◽  
Vol 95 (1) ◽  
pp. 13-16 ◽  
Author(s):  
A. Morikawa ◽  
U. Dahlgren ◽  
B. Carlsson ◽  
I. Narayanan ◽  
M. Hahn-Zoric ◽  
...  
Keyword(s):  
Breast Milk ◽  
Soybean Protein ◽  
Secretory Iga ◽  
Iga Antibodies ◽  
Japanese Mothers

Quantification of Vaccine-induced Antipertussis Toxin Secretory IgA Antibodies in Breast Milk

2015 ◽  
Vol 34 (6) ◽  
pp. e149-e152 ◽  
Author(s):  
Sara De Schutter ◽  
Kirsten Maertens ◽  
Lesley Baerts ◽  
Ingrid De Meester ◽  
Pierre Van Damme ◽  
...  
Keyword(s):  
Breast Milk ◽  
Secretory Iga ◽  
Iga Antibodies

scholarly journals Antibody in Middle Ear Fluid of Children Originates Predominantly from Sera and Nasopharyngeal Secretions

2012 ◽  
Vol 19 (10) ◽  
pp. 1593-1596 ◽  
Author(s):  
Ravinder Kaur ◽  
Thomas Kim ◽  
Janet R. Casey ◽  
Michael E. Pichichero
Keyword(s):  
Middle Ear ◽  
Acute Otitis Media ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Two Dimensional Gel Electrophoresis ◽  
Middle Ear Fluid ◽  
Iga Antibodies ◽  
Iga Antibody ◽  
Entire Array

ABSTRACTThe human middle ear is devoid of any immunocompetent cells in normal mucosa. We sought to determine the source of antibody present in the middle ear of children. Total IgG, IgA, and secretory IgA antibodies were determined by enzyme-linked immunosorbent assay from the nasopharyngeal, middle ear, and serum samples of children with acute otitis media. The two-dimensional gel electrophoresis pattern of the entire array of IgA antibodies in the nasal wash (NW) and middle ear fluid (MEF) was compared from the MEF and NW samples using isoelectric focusing and Western blotting. The total IgG and IgA antibodies in the MEF and NW samples of 137 children were compared. The ratio of IgG to IgA in the MEF was significantly different (P< 0.008) compared to NW because IgA levels were higher and IgG levels lower in NW. The IgG/IgA ratio of MEF resembled serum consistent with transudation to the MEF. Small amounts of secretory IgA were detected in MEF but the electrophoresis patterns of the entire array of IgA antibodies in the MEF and NW were virtually identical in each child evaluated; thus, IgA in MEF derived predominantly from serum and the nasopharynx by reflux via the Eustachian tube. The IgG/IgA antibody levels in the MEF and the same composition of IgA antibody in the MEF and NW identifies the predominant source of antibody in the MEF as a transudate of serum combined with nasal secretions refluxed from the nasopharynx in children.

scholarly journals Detection of cross-reactive IgA against SARS-CoV-2 spike 1 subunit in saliva

2021 ◽  
Author(s):  
Keiichi Tsukinoki ◽  
Tatsuo Yamamoto ◽  
Keisuke Handa ◽  
Mariko Iwamiya ◽  
Juri Saruta ◽  
...  
Keyword(s):  
Breast Milk ◽  
Recombinant Protein ◽  
University Hospital ◽  
Spike Protein ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Receptor Binding Domain ◽  
Blood Samples ◽  
Milk Samples ◽  
Mucosal Surfaces

AbstractAbundant secretory IgA (sIgA) in mucus, breast milk, and saliva provides immunity that prevents infection of mucosal surfaces. sIgA in pre-pandemic breast milk samples have been reported to cross-react with SARS-CoV-2, but whether it also occurs in saliva and, if so, whether it cross-reacts with SARS-CoV-2, has remained unknown. We aimed to clarify whether sIgA in saliva cross-reacts with SARS-CoV-2 spike 1 subunit in individuals who have not been infected with this virus. The study included 137 (male, n = 101; female, n = 36; mean age, 38.7 [24–65] years) of dentists and doctors in the Kanagawa Dental University Hospital. Saliva and blood samples were analyzed by PCR and immunochromatography for IgG and IgM, respectively. We then identified patients with saliva samples that were confirmed as PCR- and IgM-negative for COVID-19. Proportions of SARS-CoV-2 cross-reactive IgA-positive individuals were determined by enzyme-linked immunosorbent assay using a biotin-labeled spike S1-mFc recombinant protein covering the receptor-binding domain of SARS-CoV-2. The proportion of SARS-CoV-2 cross-reactive IgA-positive individuals was 46.7%, and this correlated negatively with age (r = −0.218, p = 0.01). The proportion of IgA-positive individuals ≥ 50 y was significantly lower than that of patients aged ≤ 49 y (p = 0.008). sIgA was purified from the saliva of all patients, and the salivary sIgA was found to suppress the binding of SARS-CoV-2 spike protein to the ACE-2 receptor. We found SARS-CoV-2 cross-reactive sIgA in the saliva of some participants who had never been infected with the virus, suggesting that sIgA helps prevent SARS-CoV-2 infection.

scholarly journals Ontogenesis of the formation of secretory antibodies to respiratory syncytial (RS) virus

1988 ◽  
Vol 101 (3) ◽  
pp. 565-575 ◽  
Author(s):  
N. P Leschinskaya ◽  
E. E Pokrovskaya ◽  
E. A Kantorovitch ◽  
S.K Grigorjeva ◽  
YA. S Shvartsman
Keyword(s):  
Old Age ◽  
Serum Antibody ◽  
Close Correlation ◽  
Secretory Iga ◽  
Igg Antibodies ◽  
Virus Infections ◽  
Neutralizing Activity ◽  
Iga Antibodies ◽  
Antibody Levels ◽  
Secretory Antibodies

SUMMARYExamination of sera from 184 children aged between 0 and 12 years and 161 adults revealed a close correlation between age and the level of humoral anti-RS virus immunity. Secretory IgG antibodies were found in children in their first months of life. Evidence for their release into secretions from the serum was obtained. This might explain the positive correlation between serum antibody levels in women recently confined with the morbidity due to RS virus in children during their first months of life. Secretory IgA antibodies were found from 4 months untill old age. The secretions of children and adults contained virus-neutralizing activity which was non-immunoglobulin in nature, as well as antibodies. However, in contrast to secretory antibody this material did not prevent development of severe RS virus infections.

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