Nonmalignant Oral Keratinocytes from Patients with Head and Neck Squamous Cell Carcinoma Show Enhanced Metabolism of Retinoic Acid

Oncology ◽  
2002 ◽  
Vol 63 (1) ◽  
pp. 56-63 ◽  
Author(s):  
Ingeborg Klaassen ◽  
Jacqueline Cloos ◽  
Serge J. Smeets ◽  
Ruud H. Brakenhoff ◽  
Maarten P. Tabor ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Retinoic Acid ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Oral Keratinocytes

scholarly journals Differences in Extracellular Vesicle Protein Cargo Are Dependent on Head and Neck Squamous Cell Carcinoma Cell of Origin and Human Papillomavirus Status

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3714
Author(s):  
Christine Goudsmit ◽  
Felipe da Veiga Leprevost ◽  
Venkatesha Basrur ◽  
Lila Peters ◽  
Alexey Nesvizhskii ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Extracellular Vesicle ◽  
Neck Squamous Cell Carcinoma ◽  
Cytokeratin 19 ◽  
Oral Keratinocytes ◽  
Transformed Cell Lines

To identify potential extracellular vesicle (EV) biomarkers in head and neck squamous cell carcinoma (HNSCC), we evaluated EV protein cargo and whole cell lysates (WCL) from HPV-positive and -negative HNSCC cell lines, as well as normal oral keratinocytes and HPV16-transformed cells. EVs were isolated from serum-depleted, conditioned cell culture media by polyethylene glycol (PEG) precipitation/ultracentrifugation. EV and WCL preparations were analyzed by LC-MS/MS. Candidate proteins detected at significantly higher levels in EV compared with WCL, or compared with EV from normal oral keratinocytes, were identified and confirmed by Wes Simple Western protein analysis. Our findings suggest that these proteins may be potential HNSCC EV markers as proteins that may be (1) selectively included in EV cargo for export from the cell as a strategy for metastasis, tumor cell survival, or modification of tumor microenvironment, or (2) representative of originating cell composition, which may be developed for diagnostic or prognostic use in clinical liquid biopsy applications. This work demonstrates that our method can be used to reliably detect EV proteins from HNSCC, normal keratinocyte, and transformed cell lines. Furthermore, this work has identified HNSCC EV protein candidates for continued evaluation, specifically tenascin-C, HLA-A, E-cadherin, EGFR, EPHA2, and cytokeratin 19.

Retinoic Acid as a Radiosensitizer on the Head and Neck Squamous Cell Carcinoma Cell Lines

2001 ◽  
Vol 33 (4) ◽  
pp. 335-342 ◽  
Author(s):  
Jae Hong Seo ◽  
Kap No Lee ◽  
Sun Hee Park ◽  
Chul Won Choi ◽  
Byung Soo Kim ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Retinoic Acid ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Carcinoma Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Squamous Cell Carcinoma Cell ◽  
Carcinoma Cell Lines

scholarly journals Early effects of cigarette smoke extract on human oral keratinocytes and carcinogenesis in head and neck squamous cell carcinoma

Head & Neck ◽  
2020 ◽  
Vol 42 (9) ◽  
pp. 2348-2354
Author(s):  
Elisabeth Foki ◽  
Katharina Gangl ◽  
Veronika Kranebitter ◽  
Verena Niederberger‐Leppin ◽  
Julia Eckl‐Dorna ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cigarette Smoke ◽  
Squamous Cell ◽  
Cigarette Smoke Extract ◽  
Neck Squamous Cell Carcinoma ◽  
Oral Keratinocytes ◽  
Early Effects
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