High-Density Oligonucleotide Array Analysis of mRNA Transcripts in Peripheral Blood Cells of Severe Atopic Dermatitis Patients

2002 ◽  
Vol 129 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Masayuki Heishi ◽  
Shinji Kagaya ◽  
Toshio Katsunuma ◽  
Toshiharu Nakajima ◽  
Keisuke Yuki ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
High Density ◽  
Oligonucleotide Array ◽  
Severe Atopic Dermatitis ◽  
Peripheral Blood Cells ◽  
Array Analysis ◽  
Mrna Transcripts ◽  
High Density Oligonucleotide Array

scholarly journals Determination of lymphocytes subpopulations in peripheral blood cells of dogs with atopic dermatitis

2013 ◽  
Vol 4 ◽  
Author(s):  
Majewska Alicja ◽  
Dembele Kourou ◽  
Gajewska Malgorzata ◽  
Motyl Tomasz
Keyword(s):  
Atopic Dermatitis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells

High-density oligonucleotide array analysis reveals extensive differences between freshly isolated blood and hepatic natural killer cells

2004 ◽  
Vol 34 (9) ◽  
pp. 2529-2540 ◽  
Author(s):  
David Vermijlen ◽  
Carine Seynaeve ◽  
Dianzhong Luo ◽  
Mogens Kruhøffer ◽  
Decio L. Eizirik ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
High Density ◽  
Oligonucleotide Array ◽  
Array Analysis ◽  
Killer Cells ◽  
High Density Oligonucleotide Array

Expression of heat shock proteins HSP90 on neutrophils peripheral blood in patients with atopic dermatitis

2016 ◽  
Vol 19 (3) ◽  
pp. 151-154
Author(s):  
I. V Elistratova ◽  
Sergey G. Morozov ◽  
I. A Zakharova
Keyword(s):  
Atopic Dermatitis ◽  
Plasma Membrane ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Cells ◽  
Significant Elevation ◽  
Hsp90 Expression ◽  
Healthy Donors ◽  
Shock Proteins

HSP90 expression on the neutrophil plasma membrane has been investigated in patients with atopic dermatitis. Methods. Human peripheral blood cells were stained with monoclonal antibodies to the HSP90 and were analyzed using the flow cytometer. Results. Neutrophils obtained from patients with atopic dermatitis have been found to express the more significant level of HSP90 on plasma membrane, and percentage of these cells has been shown to be higherfor atopic patients compared with healthy donors. Both, neutrophil count and HSP90 fluorescence were increased according elevation of index SCORAD for atopic patients. The most significant elevation in percentage of neutrophils expressing HSP90 on plasma membrane has been found during the first and second weeks of acute phase of the disease. We did not found the significant differences for HSP90 expression on neutrophils dependent on IgE level in the blood serum. Conclusion. HSP90 expression is elevated on the neutrophil plasma membrane from atopic dermatitis patients compared with healthy donors. Percentage of these cells were positive correlated with SCORAD index.

Receptor Mediated Binding of the Fibrinolytic Components, Plasminogen and Urokinase, to Peripheral Blood Cells

1987 ◽  
Vol 58 (03) ◽  
pp. 936-942 ◽  
Author(s):  
Lindsey A Miles ◽  
Edward F Plow
Keyword(s):  
T Cells ◽  
B Cell ◽  
Peripheral Blood ◽  
Binding Sites ◽  
Blood Cells ◽  
High Capacity ◽  
Red Cells ◽  
Peripheral Blood Cells ◽  
Cellular Functions ◽  
Fibrinolytic Proteins

SummaryGlu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound saturably and reversibly to monocytes, granulocytes and lymphocytes with apparent Kd values of 0.9-1.4 μM. The interactions were of high capacity with 1.6 to 49 × 105 sites/cell and involved the lysine binding sites of plasminogen. Both T cells and non-rosetting lymphocytes and two B cell lines saturably bound plasminogen. Urokinase bound saturably to gianulocytes, monocytes, non-rosetting lymphocytes and a B cell line, but minimally to T cells, platelets and red cells. Therefore, plasminogen binding sites of high capacity, of similar affinities, and with common recognition specificities are expressed by many peripheral blood cells. Urokinase receptors are also widely distributed, but less so than plasminogen binding sites. The binding ol plasminogen and/ or urokinase to these cells may lead to generation of cell- associated proteolytic activity which contributes to a variety of cellular functions.

scholarly journals RNA Sequencing of Human Peripheral Blood Cells Indicates Upregulation of Immune-Related Genes in Huntington's Disease

2020 ◽  
Vol 11 ◽  
Author(s):  
Miguel A. Andrade-Navarro ◽  
Katja Mühlenberg ◽  
Eike J. Spruth ◽  
Nancy Mah ◽  
Adrián González-López ◽  
...  
Keyword(s):  
Gene Expression ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Trinucleotide Repeat ◽  
Neurodegenerative Disorder ◽  
Gene Expression Signature ◽  
Interferon Response ◽  
Peripheral Blood Cells

Huntington's disease (HD) is an autosomal dominantly inherited neurodegenerative disorder caused by a trinucleotide repeat expansion in the Huntingtin gene. As disease-modifying therapies for HD are being developed, peripheral blood cells may be used to indicate disease progression and to monitor treatment response. In order to investigate whether gene expression changes can be found in the blood of individuals with HD that distinguish them from healthy controls, we performed transcriptome analysis by next-generation sequencing (RNA-seq). We detected a gene expression signature consistent with dysregulation of immune-related functions and inflammatory response in peripheral blood from HD cases vs. controls, including induction of the interferon response genes, IFITM3, IFI6 and IRF7. Our results suggest that it is possible to detect gene expression changes in blood samples from individuals with HD, which may reflect the immune pathology associated with the disease.

Transplantation of Bone Marrow as Compared with Peripheral-Blood Cells from HLA-Identical Relatives in Patients with Hematologic Cancers

2001 ◽  
Vol 344 (3) ◽  
pp. 175-181 ◽  
Author(s):  
William I. Bensinger ◽  
Paul J. Martin ◽  
Barry Storer ◽  
Reginald Clift ◽  
Steven J. Forman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells
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