Testicular Sperm Extraction Combined with Cryopreservation of Testicular Tissue in the Treatment of Azoospermia

2000 ◽  
Vol 65 (1) ◽  
pp. 15-20 ◽  
Author(s):  
C. Trombetta ◽  
G. Liguori ◽  
L. Gianaroli ◽  
M.C. Magli ◽  
H.A. Selman ◽  
...  
Keyword(s):  
Testicular Tissue ◽  
Testicular Sperm Extraction ◽  
Testicular Sperm

Ultrastructural And Histopathological Investigation Of Seminiferous Tubules Obtained From Azoospermia Cases In The IVF Center

2020 ◽  
pp. 1-3
Author(s):  
◽  
◽  
◽  
Keyword(s):  
Testicular Biopsy ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Testicular Sperm Extraction ◽  
Interstitial Tissue ◽  
Testicular Sperm ◽  
Electron Microscopic ◽  
Important Indicator ◽  
Serum Hormone ◽  
Histopathological Results

Objective: In this study we purposed to explore seminiferous tubules via histopathological and electron microscopic methods in testicular biopsy samples obtained TESE and the relationship between the findings and levels of serum FSH, LH, testosterone hormones. Methods: Azoospermia patients were divided into two groups, a positive testicular sperm extraction (TESE(+)) and a negative testicular sperm extraction (TESE(-)). Testicular tissue from biopsy samples were subjected to the light and electron microscopic tissue preparations. Serum hormone levels of patients were determined and analyzed statistically between the groups. Results: Compared the groups, more remarkable damages were detected in the seminiferous tubulus of no sperm group in the light and electron microscopic examinations. Although inflammation and partly tubule degeneration was observed, spermatogenesis and sperm cells were determined in the tubules of sperm pozitive group. In the light and ultrastructural analysis of negative sperm group, macrophages and mast cells in the interstitial tissue, vacuolization of seminiferous tubules, lipid inclusions and Sertoli cell only syndrome were the significant findings. When analyzed serum FSH, LH and testosterone hormones between the groups, FSH and LH hormones were statistically significant while Testosteron hormone was not significant. Conclusion: As a result in the seminiferous tubules of individuals histopathological results revealed that FSH and LH are important indicator of testicular function but Testosteron has not any effect. It was concluded that high levels of these hormones impair spermatogenesis and cause testicular failure.

scholarly journals Two Decades from the Introduction of Microdissection Testicular Sperm Extraction: How This Surgical Technique Has Improved the Management of NOA

2021 ◽  
Vol 10 (7) ◽  
pp. 1374
Author(s):  
Nahid Punjani ◽  
Caroline Kang ◽  
Peter N. Schlegel
Keyword(s):  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Testicular Sperm Extraction ◽  
Obstructive Azoospermia ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Vitro Fertilization ◽  
Surgeon Experience ◽  
Patient Optimization

The treatment of men with non-obstructive azoospermia (NOA) has improved greatly over the past two decades. This is in part due to the discovery of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), but also significantly due to improvements in surgical sperm retrieval methods, namely the development of microdissection testicular sperm extraction (mTESE). This procedure has revolutionized the field by allowing for identification of favorable seminiferous tubules while simultaneously limiting the amount of testicular tissue removed. Improving sperm retrieval rates is imperative in this cohort of infertile men as there are a limited number of factors that are predictive of successful sperm retrieval. Currently, sperm retrieval in NOA men remains dependent on surgeon experience, preoperative patient optimization and teamwork with laboratory personnel. In this review, we discuss the evolution of surgical sperm retrieval methods, review predictors of sperm retrieval success, compare and contrast the data of conventional versus mTESE, share tips for optimizing sperm retrieval outcomes, and discuss the future of sperm retrieval in men with NOA.

DAAM1 and PREP are involved in human spermatogenesis

2020 ◽  
Vol 32 (5) ◽  
pp. 484 ◽  
Author(s):  
Massimo Venditti ◽  
Chiara Fasano ◽  
Sergio Minucci ◽  
Ismene Serino ◽  
Antonio Agostino Sinisi ◽  
...  
Keyword(s):  
Male Gamete ◽  
Testicular Tissue ◽  
Testicular Sperm Extraction ◽  
Testicular Sperm ◽  
Infertile Men ◽  
New Knowledge ◽  
Cytoskeletal Dynamics ◽  
Associated Proteins ◽  
Immunofluorescent Analysis ◽  
Human Spermatogenesis

During differentiation of the male gamete, there is a massive remodelling in the shape and architecture of all the cells in the seminiferous epithelium. The cytoskeleton, as well as many associated proteins, plays a pivotal role in this process. To better characterise the factors involved, we analysed two proteins: the formin, dishevelled-associated activator of morphogenesis 1 (DAAM1), which participates in the regulation of actin polymerisation, and the protease, prolyl endopeptidase (PREP), engaged in microtubule-associated processes. In our previous studies we demonstrated their involvement in cytoskeletal dynamics necessary for correct postnatal development of the rat testis. Here, we used samples of testicular tissue obtained from infertile men by testicular sperm extraction and the spermatozoa of asthenoteratozoospermic patients. By western blot and immunofluorescent analysis, we found that DAAM1 and PREP expression and localisation were impaired in both the testis and spermatozoa, and in particular in the midpiece as well as in the principal and end-pieces of the flagella, as compared with spermatozoa of normospermic men. Our results provide new knowledge of the dynamics of spermatogenesis, raising the possibility of using DAAM1 and PREP as new markers of normal fertility.

1406: Testicular Sperm Extraction Combined with Intracytoplasmic Sperm Injection in Men with Postchemotherapy Azoospermia

2006 ◽  
Vol 175 (4S) ◽  
pp. 453-454
Author(s):  
Hiroshi Okada ◽  
Osamu Maruyama ◽  
Kojiro Nishio ◽  
Keisuke Saito ◽  
Takashi Yoshii ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Testicular Sperm Extraction ◽  
Testicular Sperm

1567: Inhibin B as A Predictor Before Testicular Sperm Extraction - Valuable Completion or Cost Factor?

2004 ◽  
Vol 171 (4S) ◽  
pp. 413-413
Author(s):  
Frank Reiher ◽  
Olrik Rau ◽  
Ingrid Nickel ◽  
Tobias Lindenmeir ◽  
Juergen Kleinstein ◽  
...  
Keyword(s):  
Inhibin B ◽  
Testicular Sperm Extraction ◽  
Testicular Sperm ◽  
Cost Factor
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