Augmentation of Methotrexate Resistance with Coexpression of Metabolically Related Genes

1999 ◽  
pp. 95-106 ◽  
Author(s):  
S. Mineishi
Keyword(s):  
Methotrexate Resistance

scholarly journals Decreased folylpolyglutamate synthetase activity as a mechanism of methotrexate resistance in CCRF-CEM human leukemia sublines.

1991 ◽  
Vol 266 (10) ◽  
pp. 6181-6187 ◽  
Author(s):  
D E McCloskey ◽  
J J McGuire ◽  
C A Russell ◽  
B G Rowan ◽  
J R Bertino ◽  
...  
Keyword(s):  
Synthetase Activity ◽  
Human Leukemia ◽  
Folylpolyglutamate Synthetase ◽  
Methotrexate Resistance

Construction and use of a dominant, selectable marker: a Harvey sarcoma virus-dihydrofolate reductase chimera

1983 ◽  
Vol 3 (1) ◽  
pp. 32-43
Author(s):  
M J Murray ◽  
R J Kaufman ◽  
S A Latt ◽  
R A Weinberg
Keyword(s):  
Long Terminal Repeat ◽  
Dihydrofolate Reductase ◽  
Human Tumor ◽  
Terminal Repeat ◽  
Biologically Active ◽  
Wild Type ◽  
Nih3t3 Cells ◽  
Exogenous Dna ◽  
Methotrexate Resistance ◽  
Sarcoma Virus

The transcriptional promoter of the Harvey sarcoma virus long terminal repeat has been used to construct a biologically active dihydrofolate reductase chimera. The construction placed the long terminal repeat at the 5' end of a dihydrofolate reductase cDNA. This chimera mediated methotrexate resistance when introduced into wild-type NIH3T3 mouse cells by transfection. The chimeric sequences were expressed in the form of polyadenylated RNA and dihydrofolate reductase protein and were amplified when the methotrexate-resistant transfectants were selected to grow in increasing methotrexate concentrations. This chimera was dominant acting and able to confer a methotrexate-resistant phenotype on wild-type NIH3T3 cells. It has been used in cotransfection experiments with DNA from human tumor cells to obtain foci of methotrexate-resistant transformed NIH3T3 cells resulting from uptake of exogenous DNA. The transfected methotrexate-resistant cells carried double minute chromosomes that appeared to contain DNA acquired during transfection.

Correlation of altered tyrosine phosphorylation with methotrexate resistance in a cisplatin-resistant subline of L1210 cells

1996 ◽  
Vol 51 (4) ◽  
pp. 477-482 ◽  
Author(s):  
Alok Bhushan ◽  
Diana Wroblewski ◽  
Yongzhi Xuan ◽  
Thomas R. Tritton ◽  
Miles P. Hacker
Keyword(s):  
Tyrosine Phosphorylation ◽  
L1210 Cells ◽  
Methotrexate Resistance

Mechanisms of Methotrexate Resistance in Acute Leukemia

1999 ◽  
pp. 543-550 ◽  
Author(s):  
Richard Gorlick ◽  
Peter Cole ◽  
Debabrata Banerjee ◽  
Giuseppe Longo ◽  
Wei Wei Li ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Methotrexate Resistance

scholarly journals The form of folate affects the mechanisms of methotrexate resistance in Enterococcus faecium

Microbiology ◽  
1998 ◽  
Vol 144 (2) ◽  
pp. 593-593
Author(s):  
T. Tamura ◽  
J. E. Baggott ◽  
K. E. Johnston ◽  
Q.-J. Li ◽  
A. C. Antony
Keyword(s):  
Enterococcus Faecium ◽  
Methotrexate Resistance

Acquired genomic aberrations associated with methotrexate resistance vary with background genomic instability

2008 ◽  
Vol 47 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Antoine M. Snijders ◽  
Mario A. Hermsen ◽  
Joshua Baughman ◽  
Tineke E. Buffart ◽  
Bing Huey ◽  
...  
Keyword(s):  
Genomic Instability ◽  
Methotrexate Resistance ◽  
Genomic Aberrations

Proteomic Analysis for the Identification of Proteins Related to Methotrexate Resistance

2006 ◽  
Vol 33 (5) ◽  
pp. 391-396 ◽  
Author(s):  
Yuan-Xi FENG ◽  
Jing BAI ◽  
Chun-Yu ZHANG ◽  
Song-Bin FU
Keyword(s):  
Proteomic Analysis ◽  
Methotrexate Resistance
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