Oncogenic Transformation of T Cells by Herpesvirus saimiri

2001 ◽  
pp. 140-158 ◽  
Author(s):  
C.M. Collins ◽  
P.G. Medveczky
Keyword(s):  
T Cells ◽  
Herpesvirus Saimiri ◽  
Oncogenic Transformation

scholarly journals Selection following isolation of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and herpesvirus saimiri-transformed T cells is comparable

2002 ◽  
Vol 83 (6) ◽  
pp. 1343-1352 ◽  
Author(s):  
Natalie N. Zheng ◽  
Cherelyn Vella ◽  
Philippa J. Easterbrook ◽  
Rod S. Daniels
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Cell Types ◽  
Herpesvirus Saimiri ◽  
Peripheral Blood Mononuclear ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells ◽  
Hiv 1

In attempts to improve isolation rates and virus yields for human immunodeficiency virus (HIV), the use of herpesvirus saimiri-immortalized T cells (HVS T cells) has been investigated as an alternative to/improvement over peripheral blood mononuclear cells (PBMCs). Here we characterize isolates rescued, in the two cell types, from two asymptomatic, long-term non-progressing HIV-1-infected individuals. All rescued viruses replicated in PBMCs and HVS T cells only, displaying a non-syncytium inducing (NSI) phenotype, and using CCR5 as co-receptor. Furthemore, PBMC/HVS T cell virus pairs displayed similar neutralization profiles. Full-length, expression-competent env genes were rescued from all virus isolates and directly from the patient samples using proviral DNA and viral RNA as templates. Compared with the sequences retrieved directly from the patient samples, both cell types showed similar selection characteristics. Whilst the selections were distinct for individual patient samples, they shared a common characteristic in selecting for viruses with increased negative charge across the V2 domain of the viral glycoproteins. The latter was observed at the env gene sequencing level for three other patients whose HIV strains were isolated in PBMCs only. This further supports a common selection for viral sequences that display a macrophage-tropic/NSI phenotype and shows that HVS T cells are a viable alternative to PBMCs for HIV-1 isolation.

scholarly journals Induction of a Novel Cellular Homolog of Interleukin-10, AK155, by Transformation of T Lymphocytes with Herpesvirus Saimiri

2000 ◽  
Vol 74 (8) ◽  
pp. 3881-3887 ◽  
Author(s):  
Andrea Knappe ◽  
Simon Hör ◽  
Sabine Wittmann ◽  
Helmut Fickenscher
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Subtractive Hybridization ◽  
Interleukin 10 ◽  
Herpesvirus Saimiri ◽  
Cell Functions ◽  
Human T Cells ◽  
Cellular Homolog ◽  
T Cell Lines

ABSTRACT Although herpesvirus saimiri-transformed T lymphocytes retain multiple normal T-cell functions, only a few changes have been described. By subtractive hybridization, we have isolated a novel cellular gene, ak155, a sequence homolog of the interleukin-10 gene. Specifically herpesvirus saimiri-transformed T cells overexpress ak155 and secrete the protein into the supernatant. In other T-cell lines and in native peripheral blood cells, but not in B cells, ak155 is transcribed at low levels. AK155 forms homodimers similarly to interleukin-10. As a lymphokine, AK155 may contribute to the transformed phenotype of human T cells after infection by herpesvirus saimiri.

scholarly journals Transformation of owl monkey T cells in vitro with Herpesvirus saimiri.

1984 ◽  
Vol 81 (14) ◽  
pp. 4563-4567 ◽  
Author(s):  
H. Rabin ◽  
R. F. Hopkins ◽  
R. C. Desrosiers ◽  
J. R. Ortaldo ◽  
J. Y. Djeu ◽  
...  
Keyword(s):  
T Cells ◽  
Herpesvirus Saimiri ◽  
Owl Monkey

scholarly journals Herpesvirus saimiri-transformed CD8+T cells as a tool to study Chediak-Higashi syndrome cytolytic lymphocytes

2005 ◽  
Vol 77 (5) ◽  
pp. 661-668 ◽  
Author(s):  
José M. Martín-Fernández ◽  
Juan A. Cabanillas ◽  
Miguel Rivero-Carmena ◽  
Esther Lacasa ◽  
Julián Pardo ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Herpesvirus Saimiri ◽  
Chediak Higashi Syndrome

scholarly journals Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells.

1997 ◽  
Vol 71 (1) ◽  
pp. 378-382 ◽  
Author(s):  
T Lund ◽  
M M Medveczky ◽  
P G Medveczky
Keyword(s):  
T Cells ◽  
Membrane Protein ◽  
Herpesvirus Saimiri

scholarly journals The superantigen-homologous viral immediate-early gene ie14/vsag in herpesvirus saimiri-transformed human T cells.

1997 ◽  
Vol 71 (12) ◽  
pp. 9124-9133 ◽  
Author(s):  
A Knappe ◽  
C Hiller ◽  
M Thurau ◽  
S Wittmann ◽  
H Hofmann ◽  
...  
Keyword(s):  
T Cells ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Immediate Early ◽  
Herpesvirus Saimiri ◽  
Human T Cells
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