Regulation of Apoptosis of Synovial Fibroblasts

2000 ◽  
pp. 216-239 ◽  
Author(s):  
J.D. Mountz ◽  
H.-G. Zhang
Keyword(s):  
Synovial Fibroblasts

The Role of Synovial Fibroblasts in Mediating Joint Destruction in Rheumatoid Arthritis

2005 ◽  
Vol 11 (5) ◽  
pp. 563-568 ◽  
Author(s):  
Ingmar Meinecke ◽  
Edita Rutkauskaite ◽  
Steffen Gay ◽  
Thomas Pap
Keyword(s):  
Rheumatoid Arthritis ◽  
Joint Destruction ◽  
Synovial Fibroblasts

FRI0020 BMP-2 and TGF-B Inhibit IL-34 Expression in Rheumatoid Arthritis Synovial Fibroblasts

2016 ◽  
Vol 75 (Suppl 2) ◽  
pp. 432.3-433
Author(s):  
M. Chemel-Mary ◽  
B. Legoff ◽  
Y. Maugars ◽  
D. Heymann ◽  
F. Verrechia
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fibroblasts

scholarly journals The activin-follistatin anti-inflammatory cycle is deregulated in synovial fibroblasts

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Magnus Diller ◽  
Klaus Frommer ◽  
Berno Dankbar ◽  
Ingo Tarner ◽  
Marie-Lisa Hülser ◽  
...  
Keyword(s):  
Synovial Fibroblasts ◽  
Anti Inflammatory

Notch‐1 and Notch‐3 Mediates Hypoxia‐Induced Synovial Fibroblasts Activation in Rheumatoid Arthritis

2021 ◽  
Author(s):  
Jianhai Chen ◽  
Wenxiang Cheng ◽  
Jian Li ◽  
Yan Wang ◽  
Jingqin Chen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fibroblasts ◽  
Notch 1 ◽  
Notch 3

scholarly journals MiR-140-3p inhibits the cell viability and promotes apoptosis of synovial fibroblasts in rheumatoid arthritis through targeting sirtuin 3

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Beibei Zu ◽  
Lin Liu ◽  
Jingya Wang ◽  
Meirong Li ◽  
Junxia Yang
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Viability ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Pearson Correlation ◽  
Fibrous Tissue ◽  
Synovial Fibroblasts ◽  
Cell Counting ◽  
Sirtuin 3 ◽  
Fibrous Tissues

Abstract Background Synovial fibroblasts (SFs) with the abnormal expressions of miRNAs are the key regulator in rheumatoid arthritis (RA). Low-expressed miR-140-3p was found in RA tissues. Therefore, we attempted to investigate the effect of miR-140-3p on SFs of RA. Methods RA and normal synovial fibrous tissue were gathered. The targets of miR-140-3p were found by bioinformatics and luciferase analysis. Correlation between the expressions of miR-140-3p with sirtuin 3 (SIRT3) was analyzed by Pearson correlation analysis. After transfection, cell viability and apoptosis were detected by cell counting kit-8 and flow cytometry. The expressions of miR-140-3p, SIRT3, Ki67, Bcl-2, Bax, and cleaved Caspase-3 were detected by RT-qPCR or western blot. Results Low expression of miR-140-3p and high expression of SIRT3 were found in RA synovial fibrous tissues. SIRT3 was a target of miR-140-3p. SIRT3 expression was negatively correlated to the expression of miR-140-3p. MiR-140-3p mimic inhibited the MH7A cell viability and the expressions of SIRT3, Ki67, and Bcl-2 and promoted the cell apoptosis and the expressions of Bax and cleaved Caspase-3; miR-140-3p inhibitor showed an opposite effect to miR-140-3p mimic on MH7A cells. SIRT3 overexpression not only promoted the cell viability and inhibited cell apoptosis of MH7A cells but also reversed the effect of miR-140-3p mimic had on MH7A cells. Conclusions The results in this study revealed that miR-140-3p could inhibit cell viability and promote apoptosis of SFs in RA through targeting SIRT3.

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