Characterization of Isolates of Bartonella henselae by Restriction Endonuclease Analysis of the PCR-Amplified 16S-23S rRNA Gene Spacer Region

1998 ◽  
pp. 154-163
Author(s):  
A.M.C. Bergmans ◽  
L.M. Schouls
Keyword(s):  
Restriction Endonuclease ◽  
Bartonella Henselae ◽  
Restriction Endonuclease Analysis ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene ◽  
Endonuclease Analysis

scholarly journals Molecular characterization of a ceftriaxone-resistant Neisseria gonorrhoeae strain found in Switzerland: a case report

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Konrad Egli ◽  
Anna Roditscheff ◽  
Ursula Flückiger ◽  
Martin Risch ◽  
Lorenz Risch ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
23S Rrna ◽  
Rrna Gene ◽  
Limited Information ◽  
Specific Pcr ◽  
Appropriate Treatment ◽  
23S Rrna Gene ◽  
Allele Type ◽  
Ciprofloxacin Resistance

Abstract Background The resistance of Neisseria gonorrhoeae to ceftriaxone is unusual in Switzerland. The underlying genotype responsible for resistance is suspected to be novel. Generally, resistance in Neisseria gonorrhoeae (Ng) involves a comprehensive set of genes with many different mutations leading to resistance to different β-lactams and fluoroquinolones. Case presentation A patient had a positive result from specific PCR for Ng. We routinely culture all clinical specimens with a positive NG-PCR. In this particular case, we isolated a strain with resistance to ceftriaxone in Switzerland. A total of seven different genes (penA, ponA, porinB, mtr, gyrA, parC, 23S rRNA gene) in this strain were partially sequenced for comparison with phenotypic susceptibility testing. Interestingly, two different mutations in the porinB gene were observed, and data on this gene are limited. Information on the identified allele type of the penA gene is very limited as well. Three different mutations of parC and gyrA that correlate with ciprofloxacin resistance were found. The combination of ceftriaxone and ciprofloxacin resistance makes an appropriate treatment difficult to obtain due to multidrug resistance. Conclusion The combined results for all genes show the appearance of new mutations in central Europe either due to worldwide spread or the emergence of new genetic combinations of mutations.

scholarly journals Genotypic Characterization of Clarithromycin-Resistant and -Susceptible Helicobacter pylori Strains from the Same Patient Demonstrates Existence of Two Unrelated Isolates

1998 ◽  
Vol 36 (9) ◽  
pp. 2730-2731 ◽  
Author(s):  
Ge Wang ◽  
Qin Jiang ◽  
Diane E. Taylor
Keyword(s):  
Helicobacter Pylori ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Pulsed Field Gel Electrophoresis ◽  
23S Rrna ◽  
Rrna Gene ◽  
Clarithromycin Resistance ◽  
23S Rrna Gene ◽  
Mode Of Development

Clarithromycin-susceptible and clarithromycin-resistantHelicobacter pylori isolates from the same patient were investigated for the mode of development and mechanism of clarithromycin resistance. The clarithromycin-resistant strain UA1182 harbors homozygous A-to-G mutations at position 2143 in both copies of the 23S rRNA gene and has a phenotype of resistance to clarithromycin and clindamycin but no significant resistance to streptogramin B. Pulsed-field gel electrophoresis patterns of NruI- andNotI-digested genomic DNA from the Clas and Clar isolates demonstrated that they are genetically distinct, suggesting that the development of clarithromycin resistance is not from the mutation of the existing Clas strain but from a completely new strain.

scholarly journals Characterization of R-plasmids coding for ampicillin resistance fromSalmonellaspecies

1979 ◽  
Vol 83 (2) ◽  
pp. 371-376
Author(s):  
A. F. Jamieson ◽  
M. J. Green
Keyword(s):  
New Zealand ◽  
Restriction Endonuclease ◽  
Restriction Endonuclease Analysis ◽  
Sudden Increase ◽  
Ampicillin Resistance ◽  
Molecular Weights ◽  
Transmissible Plasmid ◽  
R Plasmids ◽  
Endonuclease Analysis

summaryA sudden increase in the incidence of ampicillin resistance was observed amongSalmonellaspecies isolated within New Zealand in 1973–4. This increase was due mainly to the appearance and proliferation ofSalmonella newingtonandSalmonella anatumserotypes resistant to ampicillin. The plasmid complements of 14 ampicillin-resistantS. newingtonandS. anatumisolates obtained from widely separated geographical areas within New Zealand between 1973 and 1974 were characterized by agarose gel electrophoresis. Each contained one or more plasmids ranging in molecular weight from 1·4 to 42 Mdal. Eleven isolates contained a self-transmissible plasmid of 33 Mdal which encoded resistance to ampicillin. After transfer toEscherichia coli, the 33 Mdal R-plasmids from each of these isolates were shown to be identical by restriction endonuclease analysis. The remaining three strains contained ampicillin R-plasmids having molecular weights of 35, 37·5 and 42 Mdal. These plasmids were shown by restriction endonuclease analysis to be related to the 33 Mdal R-plasmid. We conclude that the 33 Mdal plasmid and its derivatives were responsible for the increase in the incidence of ampicillin-resistantS. newingtonandS. anatumserotypes among the totalSalmonellapopulation.

scholarly journals Characterization of Dutch porcine Serpulina (Treponema) isolates by restriction endonuclease analysis and DNA hybridization

1992 ◽  
Vol 138 (9) ◽  
pp. 1929-1934 ◽  
Author(s):  
A. A. H. M. T. Huurne ◽  
M. Van Houten ◽  
M. B. H. Koopman ◽  
B. A. M. Van Der Zeijst ◽  
W. Gaastra
Keyword(s):  
Restriction Endonuclease ◽  
Dna Hybridization ◽  
Restriction Endonuclease Analysis ◽  
Endonuclease Analysis

scholarly journals Identification of Nocardia veterana/elegans by Restriction Endonuclease Analysis of an Amplified Portion of 16S rRNA Gene: A Case Report

2012 ◽  
Vol 138 (suppl 1) ◽  
pp. A302-A302 ◽  
Author(s):  
Anna T. Vischio ◽  
Diana Mosteanu ◽  
Kelly Aminti ◽  
Aminti Botta ◽  
Jessica Dodge ◽  
...  
Keyword(s):  
Case Report ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Restriction Endonuclease ◽  
Restriction Endonuclease Analysis ◽  
Rrna Gene ◽  
Endonuclease Analysis
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