The Increase in Glutamine Synthesis from Glucose Caused by Ammonium Chloride in Rabbit Kidney Tubules Does Not Involve an Increase in Citrate Synthesis

1997 ◽  
pp. 19-24
Author(s):  
M.-F. Chauvin ◽  
F. Megnin-Chanet ◽  
G. Martin ◽  
J.-M. Lhoste ◽  
G. Baverel
Keyword(s):  
Ammonium Chloride ◽  
Rabbit Kidney ◽  
Kidney Tubules ◽  
Glutamine Synthesis

Chloride flux in rabbit kidney tubules in vitro

1967 ◽  
Vol 213 (5) ◽  
pp. 1249-1253 ◽  
Author(s):  
M Abramow ◽  
MB Burg ◽  
J Orloff
Keyword(s):  
Rabbit Kidney ◽  
Kidney Tubules ◽  
Chloride Flux

scholarly journals Release and fixation of CO2 by guinea-pig kidney tubules metabolizing aspartate

1992 ◽  
Vol 284 (3) ◽  
pp. 697-703
Author(s):  
G Martin ◽  
C Michoudet ◽  
N Vincent ◽  
G Baverel
Keyword(s):  
Guinea Pig ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Co2 Fixation ◽  
Carbon Oxidation ◽  
Kidney Tubules ◽  
Pig Kidney ◽  
Glutamine Synthesis ◽  
Oxoglutarate Dehydrogenase

1. The metabolism of L-[U-14C]aspartate, L-[1-14C]aspartate and L-[4-14C]aspartate was studied in isolated guinea-pig kidney tubules. 2. Oxidation of C-1 plus that of C-4 of aspartate accounted for 90-92% of the CO2 released from aspartate, whereas oxidation of the inner carbon atoms of aspartate (which occurs beyond the 2-oxoglutarate dehydrogenase step) represented only 8-10% of aspartate carbon oxidation. 3. The formation of [1-14C]glutamine and [1-14C]glutamate from [1-14C]aspartate and [4-14C]aspartate indicated that about one-third of the oxaloacetate synthesized from aspartate underwent randomization at the level of fumarate. 4. With [U-14C]aspartate as substrate, the percentage of the C-1 of glutamate and glutamine found radiolabelled after 60 min of incubation was 92.7% and 47.5% in the absence and the presence of bicarbonate respectively. 5. That CO2 fixation occurred at high rates in the presence of bicarbonate was demonstrated by incubating tubules with aspartate plus [14C]bicarbonate; under this condition, the label fixed was found in C-1 of glutamate, glutamine and aspartate, as well as in C-4 of aspartate, demonstrating not only randomization of aspartate carbon but also aspartate resynthesis secondary to oxaloacetate cycling via phosphoenolpyruvate carboxykinase, pyruvate kinase and pyruvate carboxylase. 6. The importance of CO2 fixation in glutamine synthesis from aspartate is discussed in relation to the possible role of the guinea-pig kidney in systemic acid-base regulation in vivo.

Effect of vanadate on the renal accumulation of p-aminohippurate in the rabbit kidney tubules in vitro

1981 ◽  
Vol 30 (15) ◽  
pp. 2141-2146 ◽  
Author(s):  
M.Iqbal Sheikh ◽  
Jan Maxild ◽  
Jesper V. Møller
Keyword(s):  
Rabbit Kidney ◽  
Kidney Tubules

THE PROTECTIVE EFFECT OF HALOTHANE ON ISCHEMIC ISOLATED RABBIT KIDNEY TUBULES

1988 ◽  
Vol 69 (3A) ◽  
pp. A446-A446
Author(s):  
M. J. Rice ◽  
J. H. Southard ◽  
J. A. Hjelmhaug
Keyword(s):  
Protective Effect ◽  
Rabbit Kidney ◽  
Kidney Tubules

Distribution of an ammonia load in the normal rat

1962 ◽  
Vol 203 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Adolfo Rosado ◽  
Gustavo Flores ◽  
Jaime Mora ◽  
Guillermo Soberón
Keyword(s):  
Ammonium Chloride ◽  
Hepatic Vein ◽  
Highly Efficient ◽  
Renal Pedicle ◽  
Hepatic Pedicle ◽  
Normal Rat ◽  
Glutamine Synthesis

An intraportal load of ammonium chloride was administered to normal rats. Blood from the hepatic vein, liver, muscle, and brain was analyzed for its ammonia and glutamine content at various times after the load, to determine the distribution of the ammonia injected. The effect of the ligation of the renal pedicle, of the dose administered, of the size of the animal, and of the administration of a second dose on the rate of the ammonia disappearance from the blood, and the influence of the ligation of the hepatic pedicle on the distribution of the ammonia load were also investigated. It was observed that the muscle is highly efficient in removing ammonia from the circulation; the metabolite apparently is actively retained in this tissue. When the liver is excluded the capacity of the animal to handle the ammonia load is greatly impaired. Under this condition glutamine synthesis is depressed and the ability of muscle to fix ammonia is diminished.

Increases of cell ATP produced by exogenous adenine nucleotides in isolated rabbit kidney tubules

1986 ◽  
Vol 250 (4) ◽  
pp. F720-F733 ◽  
Author(s):  
J. M. Weinberg ◽  
H. D. Humes
Keyword(s):  
Cell Injury ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Recovery Period ◽  
Nucleotide Metabolism ◽  
Rabbit Kidney ◽  
Kidney Tubules ◽  
Atp Levels ◽  
Adenine Nucleotide Metabolism ◽  
Functional Consequences

The effects of exogenous nucleotides on adenine nucleotide metabolism and cell cation levels in normal and O2-deprived isolated rabbit kidney tubules were studied to gain insight into ways in which exogenous nucleotides could contribute to ameliorating O2 deprivation-induced injury. In control oxygenated tubules, 250 microM exogenous ATP, ADP, or AMP resulted in two- to threefold increases of cell ATP over 75-90 min of incubation and smaller relative increases of ADP and AMP. GTP was not increased. Exogenous adenosine, inosine, and hypoxanthine were substantially less effective at increasing intracellular nucleotides than equimolar concentrations of exogenous nucleotides. Nucleotide-treated cells had higher levels of Ca2+ and Mg2+ than untreated cells. Treatment of O2-deprived tubules with exogenous Mg-ATP improved recovery of ATP levels following O2 deprivation, and tubules with mild injury increased their ATP levels to supranormal values such as those seen in control oxygenated tubules treated with nucleotides. Increases of tubule cell ATP levels required ongoing oxidative metabolism and thus were not evident until the reoxygenation recovery period. Exogenous ATP produced some improvement of other injury-associated metabolic parameters but did not substantially alter the overall pattern of tubule susceptibility to lethal cell injury. Allopurinol did not affect the behavior of oxygenated or O2-deprived tubules irrespective of the presence of exogenous ATP. These data clarify the potential for manipulating intracellular ATP levels with exogenous nucleotides and the functional consequences of such manipulation in oxygenated and O2-deprived renal tubule cells.

scholarly journals The Anaplerotic Substrate Alanine Stimulates Acetate Incorporation into Glutamate and Glutamine in Rabbit Kidney Tubules

2002 ◽  
Vol 277 (33) ◽  
pp. 29444-29454 ◽  
Author(s):  
Agnès Conjard ◽  
Sylvie Dugelay ◽  
Marie-France Chauvin ◽  
Daniel Durozard ◽  
Gabriel Baverel ◽  
...  
Keyword(s):  
Rabbit Kidney ◽  
Acetate Incorporation ◽  
Kidney Tubules
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