Central Role of the Transcription Factor Nuclear Factor-κB in Mesangial Cell Production of Chemokines

1997 ◽  
pp. 210-218 ◽  
Author(s):  
C. Guijarro ◽  
Y. Kim ◽  
B.L. Kasiske ◽  
Z.A. Massy ◽  
M.P. O�Donnell ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nuclear Factor Kappa B ◽  
Mesangial Cell ◽  
Nuclear Factor ◽  
Cell Production ◽  
Nuclear Factor Kappa ◽  
Transcription Factor Nuclear Factor

scholarly journals Challenges with Methods for Detecting and Studying the Transcription Factor Nuclear Factor Kappa B (NF-κB) in the Central Nervous System

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1335
Author(s):  
Marina Mostafizar ◽  
Claudia Cortes-Pérez ◽  
Wanda Snow ◽  
Jelena Djordjevic ◽  
Aida Adlimoghaddam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Signaling Pathways ◽  
Quantitative Methods ◽  
Nuclear Factor Kappa B ◽  
Inflammatory Responses ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Regulated Gene Expression ◽  
Transcription Factor Nuclear Factor

The transcription factor nuclear factor kappa B (NF-κB) is highly expressed in almost all types of cells. NF-κB is involved in many complex biological processes, in particular in immunity. The activation of the NF-κB signaling pathways is also associated with cancer, diabetes, neurological disorders and even memory. Hence, NF-κB is a central factor for understanding not only fundamental biological presence but also pathogenesis, and has been the subject of intense study in these contexts. Under healthy physiological conditions, the NF-κB pathway promotes synapse growth and synaptic plasticity in neurons, while in glia, NF-κB signaling can promote pro-inflammatory responses to injury. In addition, NF-κB promotes the maintenance and maturation of B cells regulating gene expression in a majority of diverse signaling pathways. Given this, the protein plays a predominant role in activating the mammalian immune system, where NF-κB-regulated gene expression targets processes of inflammation and host defense. Thus, an understanding of the methodological issues around its detection for localization, quantification, and mechanistic insights should have a broad interest across the molecular neuroscience community. In this review, we summarize the available methods for the proper detection and analysis of NF-κB among various brain tissues, cell types, and subcellular compartments, using both qualitative and quantitative methods. We also summarize the flexibility and performance of these experimental methods for the detection of the protein, accurate quantification in different samples, and the experimental challenges in this regard, as well as suggestions to overcome common challenges.

scholarly journals Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages

1997 ◽  
Vol 6 (3) ◽  
pp. 225-232 ◽  
Author(s):  
E. E. Mannick ◽  
J. Mishra ◽  
J. Marque ◽  
M. Clavell ◽  
M. J. S. Miller ◽  
...  
Keyword(s):  
Nuclear Factor Kappa B ◽  
Pyrrolidine Dithiocarbamate ◽  
Nuclear Factor ◽  
Chloromethyl Ketone ◽  
Nuclear Factor Kappa ◽  
Pharmacologic Inhibitors ◽  
Precise Role ◽  
Lethal Doses ◽  
Transcription Factor Nuclear Factor

The precise role of the transcription factor nuclear factor kappa B (NF- κB) in the regulation of cell survival and cell death is still unresolved and may depend on cell type and position in the cell cycle. The aim of this study was to determine if three pharmacologic inhibitors of NF-κB, pyrrolidine dithiocarbamate, N-tosyl-L-lysl chloromethyl ketone and calpain I inhibitor, induce apoptosis in a murine macrophage cell line (RAW 264.7) at doses similar to those required for NF-κB inhibition. We found that each of the three inhibitors resulted in a dose- and time-dependent increase in morphologic indices of apoptosis in unstimulated, LPS-stimulated and TNF-stimulated cells. Lethal doses were consistent with those required for NF- κB inhibition. We conclude that nuclear NF-κB activation may represent an important survival mechanism in macrophages.

scholarly journals Potential Role of Nuclear FactorκB in Diabetic Cardiomyopathy

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
O. Lorenzo ◽  
B. Picatoste ◽  
S. Ares-Carrasco ◽  
E. Ramírez ◽  
J. Egido ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Diabetic Cardiomyopathy ◽  
Nuclear Factor Kappa B ◽  
Potential Role ◽  
Heart Diseases ◽  
Cardiac Injury ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa

Diabetic cardiomyopathy entails the cardiac injury induced by diabetes independently of any vascular disease or hypertension. Some transcription factors have been proposed to control the gene program involved in the setting and development of related processes. Nuclear factor-kappa B is a pleiotropic transcription factor associated to the regulation of many heart diseases. However, the nuclear factor-kappa B role in diabetic cardiomyopathy is under investigation. In this paper, we review the nuclear factor-kappa B pathway and its role in several processes that have been linked to diabetic cardiomyopathy, such as oxidative stress, inflammation, endothelial dysfunction, fibrosis, hypertrophy and apoptosis.

The Transcription Factor Nuclear Factor-kappa B and Cancer

2007 ◽  
Vol 19 (2) ◽  
pp. 154-161 ◽  
Author(s):  
R.O. Escárcega ◽  
S. Fuentes-Alexandro ◽  
M. García-Carrasco ◽  
A. Gatica ◽  
A. Zamora
Keyword(s):  
Transcription Factor ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Transcription Factor Nuclear Factor
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