Identification and Characterisation of the IgE-Binding Proteins 2S Albumin and Conglutin γ in Almond (Prunus dulcis) Seeds

2002 ◽  
Vol 128 (2) ◽  
pp. 97-104 ◽  
Author(s):  
P. Poltronieri ◽  
M.S. Cappello ◽  
N. Dohmae ◽  
A. Conti ◽  
D. Fortunato ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Prunus Dulcis ◽  
2S Albumin ◽  
Ige Binding ◽  
Conglutin Γ

Sub-proteome analysis of novel IgE-binding proteins from Bermuda grass pollen

PROTEOMICS ◽  
2005 ◽  
Vol 5 (14) ◽  
pp. 3805-3813 ◽  
Author(s):  
Shao-Hsuan Kao ◽  
Song-Nan Su ◽  
Shih-Wen Huang ◽  
Jaw-Ji Tsai ◽  
Lu-Ping Chow
Keyword(s):  
Grass Pollen ◽  
Binding Proteins ◽  
Proteome Analysis ◽  
Bermuda Grass ◽  
Ige Binding ◽  
Bermuda Grass Pollen

Cross-Reactivity between IgE-Binding Proteins from Anisakis Simplex and Dermatophagoides Pteronyssinus

2005 ◽  
Vol 18 (4) ◽  
pp. 671-675 ◽  
Author(s):  
R. Bernardini ◽  
G. Mistrello ◽  
E. Novembre ◽  
D. Roncarolo ◽  
S. Zanotta ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Binding Proteins ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Cross Reaction ◽  
Ige Binding ◽  
Sds Page ◽  
The Cross

An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr) is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patient's serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > 100 kD, 2) against various metabolic As antigens with a mw > 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patient's serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patient's serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.

scholarly journals Goji berries superfood – contributions for the characterisation of proteome and IgE-binding proteins

2019 ◽  
Vol 30 (1) ◽  
pp. 262-280 ◽  
Author(s):  
Sandrina Teixeira ◽  
Inês M. Luís ◽  
M. Margarida Oliveira ◽  
Isabel A. Abreu ◽  
Rita Batista
Keyword(s):  
Binding Proteins ◽  
Ige Binding ◽  
Goji Berries

scholarly journals Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens

Allergy ◽  
2004 ◽  
Vol 59 (12) ◽  
pp. 1277-1284 ◽  
Author(s):  
A. L. Karlsson ◽  
R. Alm ◽  
B. Ekstrand ◽  
S. Fjelkner-Modig ◽  
A. Schiott ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Bet V 1 ◽  
Ige Binding ◽  
Bet V 1 Homologues

Comparative Analysis of IgE Binding Proteins in GM and Non-GM Rice Varieties Using Atopic Patients Sera

2013 ◽  
Vol 131 (2) ◽  
pp. AB19 ◽  
Author(s):  
Chandni Mathur ◽  
P.C. Kathuria ◽  
Shakuntala Lavasa ◽  
Pushpa Dahiya ◽  
Anand Singh
Keyword(s):  
Comparative Analysis ◽  
Binding Proteins ◽  
Rice Varieties ◽  
Ige Binding ◽  
Gm Rice
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