Current Serological and Molecular Methods in the Diagnosis of Systemic Infections with Candida sp. and Aspergillus sp. in Immunocompromised Patients with Hematological Malignancies

1999 ◽  
pp. 59-68
Author(s):  
D. Buchheidt ◽  
H. Skladny ◽  
C. Baust ◽  
R. Hehlmann
Keyword(s):  
Hematological Malignancies ◽  
Molecular Methods ◽  
Immunocompromised Patients ◽  
Candida Sp ◽  
Aspergillus Sp ◽  
Systemic Infections

scholarly journals Pyrrolocin A, a 3-Decalinoyltetramic Acid with Selective Biological Activity, Isolated from Amazonian Cultures of the Novel Endophyte Diaporthales sp. E6927E

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001
Author(s):  
Eric V. Patridge ◽  
Alicia Darnell ◽  
Kaury Kucera ◽  
Gillian M. Phillips ◽  
Heidi R. Bokesch ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Inhibitory Concentration ◽  
Cd Spectroscopy ◽  
Fungal Endophyte ◽  
Immunocompromised Patients ◽  
Drug Resistant ◽  
The Novel ◽  
Tetramic Acid ◽  
Candida Sp ◽  
Aspergillus Sp

Natural products remain an important source of new therapeutics for emerging drug-resistant pathogens like Candida albicans, which particularly affects immunocompromised patients. A bioactive 3-decalinoyltetramic acid, pyrrolocin A, was isolated from extracts of a novel Amazonian fungal endophyte, E6927E, of the Diaporthales family. The structure of the natural product was solved using NMR and CD spectroscopy and it is structurally related to the fungal setins, equisetin and phomasetin, which are well-characterized tetramic acid antibiotics specific for Gram-positive organisms. We show that the compound inhibits growth of Staphylococcus aureus and Enterococcus faecalis. It shows selective and potent bioactivity against fungal strains, with an MIC of 4 μg/mL for C. albicans, 100 μg/mL for Aspergillus sp. and greater than 100 μg/mL for Saccharomyces cerevisiae. Further, the compound is less toxic to mammalian cells (IC50 = 150 μg/mL), with an inhibitory concentration greater than forty times that for C. albicans. Pyrrolocin A retained potent activity against eight out of seventeen strains of clinical Candida sp. isolates tested.

scholarly journals Air Quality Assessment of Port Harcourt Urban Slums and Health Implications

2021 ◽  
pp. 50-57
Author(s):  
H. O. Stanley ◽  
M. E. Amesi
Keyword(s):  
Air Quality ◽  
Fungal Species ◽  
Urban Slums ◽  
Candida Sp ◽  
Suspended Particulate ◽  
Port Harcourt ◽  
Microbiological Parameters ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

This study was conducted to assess the outdoor air quality of some urban slums in Port Harcourt. Six sampling sites were selected, from the Port Harcourt urban slums; two sites from each slum represented with a suffix 1 or 2.  The slums are designated Marine base (#1 and #2), RSU BG, Obudu 2, Bundu (#1 and #2). The air quality was analyzed using portable handheld air quality analyzer and the microbiological parameters were determined by standard cultural method. The study revealed that the sampled sites were laden with bacterial and fungal species. namely; Klebsiella sp., Micrococcus sp., Escherichia sp., Pseudomonas sp., Baccilus sp., Aeromonas sp., Streptococus sp., Serratia sp., Aerococcus sp., Proteus sp. Penicillium sp., Fusarium sp., Candida sp., Aspergillus sp., Mucor sp., Rhizopus sp. and Tricorderma sp. Highest obtained noise level was at Marine base 1 which was  66 db, highest relative humidity of 54.8% at RSU BG, CO2  (ppm) values of 4.8, 80, 796, 850, 638, 698 for Marine base 2, Marine base 1, Obudu 2, RSU BG, Bundu 1 and Bundu 2 respectively. The values for NO2 (ppm) was (0.05, 0.053, 0.071, 0.022, 0.035, 0.023), suspended particulate matter (ppm) was (7.1, 8.7, 9.5, 9.5, 6.2, 6.2), SO2 (ppm) was (0.42, 0.15, 0.50, 0.34, 1.26, 0.41) CO (ppm) was (4.8, 1.7, 2.2, 3.0, 3.9, 3.6) and volatile organic compound (ppm) was (1.0, 1.1, 0.9, 75 and 1.2). This study has shown that Port Harcourt urban slums are experiencing some degree of contamination not acceptable for healthy living that requires attention to curb. These areas require all-round improvement in sanitation.   M Give one sentence on methodology.

Grupos de fungos e bactérias isolados no trato respiratório de aves marinhas em reabilitação na região costeira da Baixada Santista

2014 ◽  
Vol 3 (1) ◽  
pp. 14-25
Author(s):  
Bruna Del Busso Zampieri ◽  
Andrea Maranho ◽  
Ana Julia Fernandes Cardoso de Oliveira
Keyword(s):  
Candida Sp ◽  
Penicillium Sp ◽  
Aspergillus Sp

O Brasil apresenta diversidade representativa de aves marinhas residentes e migratórias. Muitas dessas aves são encontradas debilitadas nas praias e são levadas para centros de reabilitação para tratamento e posterior soltura. Algumas dessas aves marinhas, devido ao aumento da poluição costeira e próprio estresse do manejo e do cativeiro, podem ser acometidas por doenças bacterianas e fungicas. Dessa maneira, o presente trabalho tem como objetivo caracterizar as principais espécies de fungos e bactérias presentes no trato respiratório de aves marinhas encontradas debilitadas na região costeira da Baixada Santista. Através das analises microbiológicas foram encontrados seis gêneros principais de bactérias: Staphylococcus sp, Streptococcus sp, Salmonella sp, Enterococcus sp e Clostridium sp e quatro de fungos: Candida sp, Aspergillus sp, Penicillium sp, Trichophyton sp. Grande parte desses microrganismos faz parte da microbiota desses animais, mas quando existe uma queda na resistência devido ao estresse causado pela migração, falta de alimento, ou mesmo durante o manejo, podem se tornar doenças graves que se não tratadas podem levar a morte das aves. Por isso, é necessário um diagnóstico prévio, para um tratamento eficaz, fazendo que o sucesso na reintrodução ou realocação seja maior contribuindo assim para conservação das espécies.

Microbiological Evaluation of Ready-to-Drink Tigernut Drinks Sold within Port Harcourt Metropolis, Rivers State, Nigeria

2020 ◽  
pp. 45-58
Author(s):  
Francis Sopuruchukwu Ire ◽  
Goziem Kim Benneth ◽  
Ndukwe Maduka
Keyword(s):  
Bacterial Count ◽  
Human Consumption ◽  
National Agency ◽  
Candida Sp ◽  
Penicillium Sp ◽  
Port Harcourt ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
And Control ◽  
Corynebacterium Sp

Aims: Tigernut drink are made from tigernut tubers (Cyperus esculentus L.) and rich in nutrients. This drink is locally produced and widely consumed in Nigeria irrespective of social status. This study is aimed at evaluating the microbial quality and physicochemical property of tigernut drinks sold within Port Harcourt metropolis. Methodology: Thirty (30) samples of freshly prepared and packaged tigernut drinks were randomly purchased from different vendors in five locations of Port Harcourt metropolis (Agip Estate, Abuja Campus (Uniport), Choba, Mile 1 and Mile 2 Markets). The samples were analyzed using standard microbiological and physicochemical methods. SPSS (Statistical Package for the Social Sciences) was used to analyze the data. Results: Results obtained showed that the pH of the samples ranged from 4.2 to 4.6 while the total heterotrophic bacterial count ranged from 6. 54-6.74 log10 CFU/mL. Total fungal count of tigernut drinks ranged from 6.0-6.2 log10 CFU/mL. A total of nine (9) bacterial genera namely Staphylococcus sp. (37.3%), Escherichia sp. (21.3%), Salmonella sp. (12%), Pseudomonas sp. (12%), Klebsiella sp. (4%), Bacillus sp. (4%), Micrococcus sp. (4%), Enterobacter sp. (2.7%) and Corynebacterium sp. (2.7%) were isolated from the samples. Six (6) fungal genera were also encountered in the drink sampled which include Rhizopus sp. (1.4%), Saccharomyces sp. (4.4%), Aspergillus sp. (30.9%), Fusarium sp. (26.5%), Penicillium sp. (30.9%) and Candida sp. (5.9%). The result revealed that Staphylococcus sp. had the highest percentage of occurrence (37.3%) followed by E. coli (21.3%), while Enterobacter sp. (2.7%) and Corynebacterium sp. (2.7%) recorded the least. Among the fungal isolates, Aspergillus sp. and Penicillium sp. had the highest percentage of occurrence (30.9%) whereas Rhizopus sp. had the least (1.4%). The results of this study revealed that all the samples from the five (5) locations were heavily contaminated with pathogenic microorganisms and found not suitable for human consumption based on the standard recommended by National Agency for Food and Drug Administration and Control (NAFDAC). NAFDAC stipulated that mesophilic aerobic count of locally prepared beverages should be < 5.0 log10 CFU/mL. Conclusion: The huge contamination recorded in all the samples irrespective of the location could be linked to poor hygienic levels during processing. Therefore, good manufacturing practices, public health enlightenment campaign and strict regulations from relevant agencies are recommended to avoid foodborne infections, diseases and possible deaths which could result from consumption of such contaminated tigernut drinks.

scholarly journals Anasarca, Fever, Thrombocytopenia, Organomegaly, and Multiorgan Failure in a 24-Year-Old Pregnant Woman

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Guillaume Morel ◽  
Joy Mootien ◽  
Philippe Guiot ◽  
Khaldoun Kuteifan
Keyword(s):  
Mechanical Ventilation ◽  
Pregnant Woman ◽  
Cesarean Section ◽  
Hematological Malignancies ◽  
Multiorgan Failure ◽  
Castleman Disease ◽  
Tafro Syndrome ◽  
Multicentric Castleman Disease ◽  
First Case ◽  
Systemic Infections

TAFRO syndrome is a distinct idiopathic multicentric Castleman disease characterized by the association of thrombocytopenia, anasarca, fever, reticulin fibrosis, and organomegaly. We report the first case occurring in a Caucasian pregnant woman. At 34 weeks of gestation, our patient presented with all clinical and biological symptoms compatible with a TAFRO syndrome. Tough quick cesarean section was performed as symptoms got worse with onset of multiorgan failure requiring mechanical ventilation for acute respiratory distress, continuous renal replacement, and vasopressors. Nine days after ICU admission, steroid boluses were started and allowed spectacular clinical and biological improvement. As systemic inflammatory manifestations are important, TAFRO syndrome can be mistaken with severe autoimmune diseases, systemic infections, hematological malignancies, or hemophagocytic lymphohistiocytosis.

scholarly journals Growth of Candida sp and Aspergillus sp from Bronchoscopy Pulmonary Tuberculosis Patients on Bran Media

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Mujahidah Basarang ◽  
Muhammad Rifo Rianto
Keyword(s):  
Pulmonary Tuberculosis ◽  
Fungal Growth ◽  
Raw Material ◽  
Growth Media ◽  
Carbohydrate Source ◽  
Tuberculosis Patients ◽  
Culture Techniques ◽  
Candida Sp ◽  
Untreated Culture ◽  
Aspergillus Sp

Pulmonary tuberculosis caused by Mycobacterium tuberculosis become ahealth problem in Indonesia. The chronic nature of this disease is further exacerbated ifit is accompanied by fungal infection such as Candida albicans and Aspergillus sp.,which is usually remains undiagnosed and thus untreated. Culture techniques can beused to identify Candida sp and Aspergillus sp from bronchoscopy. Fungal culturemedia in laboratory containing high carbohydrate source, nitrogen source are requiredfor the growth. This nutrient can be found in bran that contains high carbohydrates,proteins, fats, vitamins, and crude fiber. So that bran can be used as raw material for alternative fungal growth media. The purpose of this study was to increase bran as amedium for the growth of Candida sp and Aspergillus sp isolated from bronchoscopy ofpulmonary TB patients. This study included bran collection, preparation of bran media,inoculation bronchoscopy on bran media, observation of fungal growth. Colonies ofCandida sp and Aspergillus sp were confirmed microscopically. The results showed thatCandida sp and Aspergillus sp grew on both media, Bekatul Dextrose Agar and PotatoDextrose Agar. The conclusion of this study is that bran can be used as a medium forfungal growth. Bran media can be used as an alternative media to replace syntheticmedia to grow Candida sp and Aspergillus sp isolated from bronchial rinses.

scholarly journals Qualidade microbiológica do ar em ambiente de um instituto de oncologia e radioterapia do município de Porto Velho

2017 ◽  
Vol 6 (2) ◽  
pp. 54
Author(s):  
Renata Maiara Afonso Cunha ◽  
Elton Bill Amaral de Souza ◽  
Helen Queite Guterres Barros Gazola
Keyword(s):  
Candida Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Porto Velho

Levando em consideração a relevância para a saúde de pacientes em internação hospitalar e profissionais presentes nessas instituições de saúde que ocupam esses ambientes climatizados por meio de ar condicionado, este trabalho tem como objetivo identificar os fungos encontrados e indicar os riscos que eles podem causar em pacientes em tratamento de câncer por meio da qualidade microbiológica do ar do Instituto de Oncologia e Radioterapia do município de Porto Velho/RO. Foi utilizado para a coleta de amostras a técnica de sedimentação espontânea realizando quatro coletas a cada 20 dias (ano de 2015) nos 4 setores, na qual a Placa 1 foi exposta por 15 minutos; a Placa 2 por 25 minutos e a Placa 3 por 30 minutos em pontos estratégicos a 1,5 metros do piso, no centro destes determinados setores, em horários que apresentavam um grande número de pessoas circulando. Foram identificados a presença de nove gêneros de fungos como o Acremonium sp., Aspergillus sp., Candida sp., Cladosporium sp., Curvularia sp., Fusarium sp., Geotrichum sp., Trichosporon sp. e Rhizopus sp. que representam grande potencialidade de infecções em pacientes imunocomprometidos, como os pacientes em tratamento de câncer, sendo o Arpergillus sp. presente em todos os setores do instituto, com isto é necessário o monitoramento contínuo do ar interior e aparelhos de ar condicionado para impedir infecções nosocomiais principalmente de pacientes em tratamento quimioterápico e de radioterapia.

scholarly journals MYCOBIOTA OF SOILS OF URBAN TERRITORIES WITH DIFFERENT LEVELS OF ANTHROPOGENIC LOAD

2019 ◽  
Vol 96 (6) ◽  
pp. 505-508
Author(s):  
Elena V. Khaldeeva ◽  
A. A. Bayazitova ◽  
S. A. Lisovskaya ◽  
N. I. Glushko ◽  
V. R. Parshakov
Keyword(s):  
Urban Environment ◽  
Trichoderma Viride ◽  
Hazard Index ◽  
Anthropogenic Load ◽  
Average Value ◽  
Candida Sp ◽  
Transport Zone ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Different Levels

Anthropogenic forcing has a huge impact on the formation of the ecosystem of modern cities, including the composition of the mycobiota of the urban environment. The paper presents the results of mycological studies of soil samples taken in the city of Kazan in areas divided into recreational and transport functional areas with different levels of anthropogenic load. More than 60 species of fungi belonging to 15 genera, including 20 species common to soils in both zones were identified. Penicillium sp., Fusarium sp., Rhizomucor sp., Trichoderma viride were shown to dominate in undisturbed natural areas, and in soils of the urban environment there was much more often found the presence of Alternaria sp., Aspergillus sp., Candida sp., Rhizopus sp., Trichophyton sp. In areas with good natural light the percentage of samples with low contamination by fungi was established to be significantly higher than in shaded areas: 16.7% and 7.1% in the recreational area; and 12.5% and 0% in the transport zone correspondingly. Potentially pathogenic species of fungi were detected in 80% of samples of recreational and in 95% of samples of transport areas. Dark-colored species (including Aspergillus, Alternaria, etc.) were shown to be more common in soils of transport zone, and Trichophyton sp. and Scopulariopsis sp. - in soils of recreational zone. The evaluation of mycological hazard index allowed to estimate the condition of the soil of Kazan as satisfactory for 80% of sites of recreational and 65% - of the transport area. The average value of the mycological hazard index for recreational areas was 2.7 and for the transport areas - 4.2. Results indicate to regular mycological monitoring would be desirable for the normalization of the mycological environment, significantly reducing the risk of mycosis, mycotoxicosis and mycogenic allergies, and should help to ensure sanitary-epidemiological welfare of the population.

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