Effects of Vitamin K2 on Bone of Ovariectomized Rats and on a Rat Osteoblastic Cell Line

2002 ◽  
Vol 53 (3) ◽  
pp. 144-148 ◽  
Author(s):  
Ichiro Iwamoto ◽  
Shoichiro Kosha ◽  
Toshinori Fujino ◽  
Yukihiro Nagata
Keyword(s):  
Cell Line ◽  
Vitamin K ◽  
Ovariectomized Rats ◽  
Osteoblastic Cell ◽  
Osteoblastic Cell Line

scholarly journals Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio

2021 ◽  
Vol 9 (4) ◽  
pp. 673
Author(s):  
Jiah Yeom ◽  
Seongho Ma ◽  
Young-Hee Lim
Keyword(s):  
Signaling Pathway ◽  
Osteoblast Differentiation ◽  
Surface Proteins ◽  
Bone Morphogenetic Protein 2 ◽  
Ovariectomized Rats ◽  
Osteoblastic Cell ◽  
Enhancement Effect ◽  
Propionibacterium Freudenreichii ◽  
Alizarin Red ◽  
Osteoblastic Cell Line

Osteoblast differentiation is important for the development of bone and the maintenance of bone density. Propionibacterium freudenreichii is a probiotic with an anti-inflammatory property. The aim of this study was to investigate the enhancement effect of P. freudenreichii MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real-time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast mineralization. Heat-killed MJ2 (hkMJ2)-treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt-related transcription factor 2. Additionally, oral administration of live or heat-killed MJ2 to ovariectomized rats inhibited osteoporosis-induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and mineralization. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.

scholarly journals Prostaglandin E receptor subtypes in mouse osteoblastic cell line.

Endocrinology ◽  
1996 ◽  
Vol 137 (5) ◽  
pp. 1698-1705 ◽  
Author(s):  
M Suda ◽  
K Tanaka ◽  
K Natsui ◽  
T Usui ◽  
I Tanaka ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteoblastic Cell ◽  
Prostaglandin E ◽  
Receptor Subtypes ◽  
Osteoblastic Cell Line

scholarly journals EMMPRIN Inhibits bFGF-Induced IL-6 Secretion in an Osteoblastic Cell Line, MC3T3-E1

2017 ◽  
Vol 14 (12) ◽  
pp. 1173-1180 ◽  
Author(s):  
Akari Saiki ◽  
Mitsuru Motoyoshi ◽  
Keiko Motozawa ◽  
Teinosuke Okamura ◽  
Kousuke Ueki ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteoblastic Cell ◽  
Osteoblastic Cell Line

An Electrochemical Assay for Monitoring Differentiation of the Osteoblastic Cell Line (MBA-15) on the Sensor Chip

2015 ◽  
Vol 27 (6) ◽  
pp. 1350-1358 ◽  
Author(s):  
Cigdem Yildirim-Semerci ◽  
Dafna Benayahu ◽  
Miriam Adamovski ◽  
Ulla Wollenberger
Keyword(s):  
Cell Line ◽  
Sensor Chip ◽  
Osteoblastic Cell ◽  
Electrochemical Assay ◽  
Osteoblastic Cell Line

Phenotypic stability of the human MG-63 osteoblastic cell line at different passages

2018 ◽  
Vol 43 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Susanne Staehlke ◽  
Henrike Rebl ◽  
Barbara Nebe
Keyword(s):  
Cell Line ◽  
Osteoblastic Cell ◽  
Phenotypic Stability ◽  
Osteoblastic Cell Line

Experimental Study of Disodium Etidronate for the Growth of MC3T3-E1 Osteoblastic Cell Line

1990 ◽  
Vol 25 (2) ◽  
pp. 553
Author(s):  
Myung Chul Yoo ◽  
Jin Hwan Ahn ◽  
Jung Soo Han ◽  
Koong Hee Cho ◽  
Byung Soon Kim
Keyword(s):  
Experimental Study ◽  
Cell Line ◽  
Osteoblastic Cell ◽  
Osteoblastic Cell Line
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