Assignment1 of maltase glucoamylase (MGAM) to pig chromosome 2 (2q21) by fluorescence in situ hybridization and confirmation by genetic mapping

2000 ◽  
Vol 90 (3-4) ◽  
pp. 236-237 ◽  
Author(s):  
J.H. Calvo ◽  
N.L. Lopez-Corrales ◽  
R. Osta ◽  
T.M. Skinner ◽  
S.I. Anderson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome 2

Refined physical mapping of the Sec-1 locus on the satellite of chromosome 1R of rye (Secale cereale)

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 889-893 ◽  
Author(s):  
W. Busch ◽  
R. G. Herrmann ◽  
R. Martin
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Fluorescence In Situ Hybridization ◽  
Physical Mapping ◽  
Secale Cereale ◽  
Secondary Constriction ◽  
Heterologous Hybridization ◽  
Secale Cereale L ◽  
Chromosome 1R

The Sec-1 locus (ω-secalin) of rye (Secale cereale L.) was mapped in the satellite of the short arm of chromosome 1R using fluorescence in situ hybridization and a genomic probe called pSec2B. Sec-1 is located in the middle of the satellite at the junction of the proximal euchromatic and the distal heterochromatic regions. Double hybridization experiments using rDNA and pSec2B showed that the NOR spans over the secondary constriction of the short arm of chromosome 1R and that there is a clearly visible gap between the NOR and Sec-1. Heterologous hybridization of pSec2B to barley visualized the B-hordein locus on chromosome 1H.Key words: fluorescence in situ hybridization, physical mapping, genetic mapping, secalin, rye, B-hordein, rDNA.

Direct and sensitive fluorescence in situ hybridization of 45S rDNA on tomato chromosomes

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1062-1065 ◽  
Author(s):  
Jie Xu ◽  
E. D. Earle
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome 2 ◽  
45S Rdna ◽  
Plant Chromosomes ◽  
Signal Sensitivity ◽  
Direct Fluorescence ◽  
Hybridization Protocol ◽  
Tomato Genotypes

We describe a direct and sensitive fluorescence in situ hybridization protocol for plant chromosomes. We labelled 45S rDNA with fluorescein-12-dUTP and hybridized to somatic chromosomes of four tomato genotypes. This technique does not require posthybridization immunocytochemical amplifications. The improved signal sensitivity with this technique allowed identification of new rDNA loci on three pairs of chromosomes, in addition to the previously known locus on chromosome 2. We discuss favorable features of direct fluorescence in situ hybridization for chromosomes fixed on a slide and chromosomes or cells in suspension.Key words: direct fluorescence in situ hybridization, 45S rDNA, tomato chromosomes.

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