DNA Topoisomerase II-Alpha Immunoreactivity as a Marker of Tumor Aggressiveness in Invasive Breast Cancer

Pathobiology ◽  
2000 ◽  
Vol 68 (3) ◽  
pp. 137-143 ◽  
Author(s):  
Lydia Nakopoulou ◽  
Andreas C. Lazaris ◽  
Nikolaos Kavantzas ◽  
Paraskevi Alexandrou ◽  
Pauline Athanassiadou ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Invasive Breast Cancer ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Tumor Aggressiveness ◽  
Topoisomerase Ii Alpha ◽  
Dna Topoisomerase

The Prognostic Significance of DNA Topoisomerase II-alpha (Ki-S1), p21/Cip-1, and p27/Kip-1 Protein Immunoexpression in Oligodendrogliomas

2001 ◽  
Vol 125 (7) ◽  
pp. 892-898 ◽  
Author(s):  
Andrey Korshunov ◽  
Andrey Golanov
Keyword(s):  
Topoisomerase Ii ◽  
World Health ◽  
Low Grade ◽  
Image Analyzer ◽  
High Grade ◽  
Dna Topoisomerase Ii ◽  
Survival Times ◽  
Topoisomerase Ii Alpha ◽  
Dna Topoisomerase ◽  
The Mean

Abstract Objective.—To evaluate a possible association between clinical outcome of patients with oligodendroglioma and expression of 2 cyclin-dependent kinase inhibitors, p21/Cip-1 (p21) and p27/Kip-1 (p27), and of DNA topoisomerase II-alpha (Ki-S1), which has been recently used as a marker of cellular proliferation. Design.—Ninety-one specially selected patients with cerebral oligodendrogliomas treated with surgery and radiotherapy were studied retrospectively. Tumor specimens were immunohistochemically examined with antibodies to p21, p27, and Ki-S1. A computerized color image analyzer was used to count immunostained nuclei. Results.—The mean Ki-S1 labeling index (LI) was found to be significantly prominent for World Health Organization (WHO) high-grade tumors (9.5% vs 3.2% for WHO low-grade tumors). In contrast, the mean p27 LI was significantly higher for low-grade tumors (43.3% vs 25.7% for high-grade tumors). The number of p21-positive cases and the mean p21 LI were found to be relatively equal for low- and high-grade tumors. For low-grade oligodendrogliomas, the progression-free and overall survival times were found to be significantly shorter for tumors with p27 LIs less than 20%. For high-grade oligodendrogliomas, survival times were significantly reduced for tumors with Ki-S1 LIs greater than 10%. Regression-tree analysis identified 4 groups of oligodendrogliomas with distinctly different outcomes: (1) 32 patients with low-grade tumors and p27 LIs greater than 20%; (2) 14 patients with low-grade tumors and p27 LIs less than 20%; (3) 25 patients with high-grade tumors and Ki-S1 LIs less than 10%; and (4) 20 patients with high-grade tumors and Ki-S1 LIs greater than 10%. Conclusions.—Immunoreactivity for Ki-S1 and p27 was found to be useful for further subdividing oligodendroglioma prognoses among low-grade and high-grade tumors. It seems unlikely that p21 immunohistochemistry will be of value for determining clinical outcomes for patients with oligodendrogliomas.

Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis

2011 ◽  
Vol 363 (1-2) ◽  
pp. 301-307 ◽  
Author(s):  
Miroslav Vařecha ◽  
Michaela Potěšilová ◽  
Pavel Matula ◽  
Michal Kozubek
Keyword(s):  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Topoisomerase Ii Alpha ◽  
Dna Topoisomerase ◽  
Histone H2b ◽  
Endonuclease G
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