Eosinophil Infiltration and Degranulation in Normal Human Tissues: Evidence for Eosinophil Degranulation in Normal Gastrointestinal Tract

2001 ◽  
Vol 125 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Masahiko Kato ◽  
Gail M. Kephart ◽  
Akihiro Morikawa ◽  
Gerald J. Gleich
Keyword(s):  
Gastrointestinal Tract ◽  
Eosinophil Infiltration ◽  
Human Tissues ◽  
Normal Human ◽  
Eosinophil Degranulation

scholarly journals Expression of TAL-1 proteins in human tissues

Blood ◽  
1995 ◽  
Vol 85 (3) ◽  
pp. 675-684 ◽  
Author(s):  
K Pulford ◽  
N Lecointe ◽  
K Leroy-Viard ◽  
M Jones ◽  
D Mathieu-Mahul ◽  
...  
Keyword(s):  
Molecular Weight ◽  
T Cell ◽  
Cell Lines ◽  
Fetal Liver ◽  
Cell Types ◽  
Human Tissues ◽  
Aberrant Expression ◽  
Erythroid Precursor ◽  
Normal Human

Rearrangement of the tal-1 gene (also known as SCL or TCL-5) occurs in at least 25% of T-cell acute lymphoblastic leukemias (T-ALLs) and results in the aberrant expression of tal-1 mRNA in the neoplastic cells. Also, tal-1 mRNA is constitutively expressed in erythroid precursors and megakaryocytes. This report describes a direct immunocytochemical study of the distribution and localization of TAL-1 protein in normal human tissues and cell lines using four monoclonal antibodies raised against recombinant TAL-1 proteins. One of these reagents recognizes a protein of 41 kD molecular weight in in vitro- translated TAL-1 proteins, two others recognize proteins of 39 and 41 kD molecular weight, and the fourth antibody also recognizes a TAL-1 protein of 22 kD in addition to the 39- and 41-kD proteins. These anti- TAL-1 antibodies label the nuclei of erythroid precursor cells and megakaryocytes in fetal liver and adult bone marrow. The punctate pattern of nuclear labeling suggests that TAL-1 may comprise part of a novel nuclear structure, similar to that recently found for the PML protein. The nuclei of T cell lines known to express mRNA encoding the full-length TAL-1 protein (eg, CCRF-CEM, RPMI 8402, and Jurkat) are also labeled. A study of normal human tissues (including thymus) showed labeling of smooth muscle, some tissue macrophages, and endothelial cells. TAL-1 protein is undetectable in other cell types. These reagents may play an important role in the diagnosis of T-ALL and could also be used in the context of lymphoma diagnosis on routinely fixed material.

scholarly journals Quantification of prolactin receptor mRNA in multiple human tissues and cancer cell lines by real time RT-PCR

2001 ◽  
Vol 171 (1) ◽  
pp. R1-R4 ◽  
Author(s):  
SK Peirce ◽  
WY Chen ◽  
WY Chen
Keyword(s):  
Breast Cancer ◽  
Prostate Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Normal Breast ◽  
Breast Cancer Cell Lines ◽  
Human Tissues ◽  
Normal Human

Human prolactin (hPRL) has been reported to be involved in breast and prostate cancer development. The hPRL receptor (hPRLR) is expressed in a wide variety of tissues in at least three isoforms. In this study, a one-step real time reverse transcription PCR technique was used to determine relative expression levels of hPRLR mRNA in eleven human breast cancer cell lines, HeLa cells, three prostate cancer cell lines and nine normal human tissues. The housekeeping gene beta-actin was used for internal normalization. We demonstrate that hPRLR mRNA is up-regulated in six of the eleven breast cancer cell lines tested when compared with normal breast tissue. Of the cancer cell lines tested, we found that T-47D cells have the highest level of hPRLR mRNA, followed by MDA-MB-134, BT-483, BT-474, MCF-7 and MDA-MB-453 cells. In two breast cancer cell lines (MDA-MB-468 and BT-549), the hPRLR levels were found to be comparable to that of normal breast tissue. Three breast cancer cell lines (MDA-MB-436, MDA-MB-157 and MDA-MB-231) expressed hPRLR mRNA at levels lower than that of normal tissue. In contrast, in all three commonly used prostate cancer cell lines (LNCaP, PC-3 and DU 145), the levels of hPRLR mRNA were found to be down-regulated relative to that of normal prostate tissue. Of nine normal human tissues tested, we found that the uterus and the breast have the highest levels of hPRLR mRNA, followed by the kidney, the liver, the prostate and the ovary. The levels of hPRLR mRNA were the lowest among the trachea, the brain and the lung.

Protein phosphatase 2Cα expression in normal human tissues: an immunohistochemical study

2001 ◽  
Vol 116 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Beatriz Lifschitz-Mercer ◽  
Yuri Sheinin ◽  
Daniella Ben-Meir ◽  
Letizia Bramante-Schreiber ◽  
Leonor Leider-Trejo ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Immunohistochemical Study ◽  
Human Tissues ◽  
Normal Human

Widespread expression of perilipin 5 in normal human tissues and in diseases is restricted to distinct lipid droplet subpopulations

2018 ◽  
Vol 374 (1) ◽  
pp. 121-136 ◽  
Author(s):  
Merita Hashani ◽  
Hagen Roland Witzel ◽  
Lena Maria Pawella ◽  
Judith Lehmann-Koch ◽  
Jens Schumacher ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Human Tissues ◽  
Normal Human ◽  
Perilipin 5
Sign in / Sign up

Export Citation Format

Share Document