Ex vivo Expansion of Umbilical Cord Blood Cells Generated from CD34+ Progenitors by Using a Megakaryocyte-Active Cytokine Combination

2001 ◽  
Vol 28 (6) ◽  
pp. 318-325
Author(s):  
H. Eichler ◽  
W. Zieger ◽  
H. Klüter
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells ◽  
Cytokine Combination

Purification of CD34+ Cells Is Essential for Optimal Ex Vivo Expansion of Umbilical Cord Blood Cells

1997 ◽  
Vol 6 (2) ◽  
pp. 145-150 ◽  
Author(s):  
ROBERT A. BRIDDELL ◽  
BRENT P. KERN ◽  
KERRY L. ZILM ◽  
GREGORY B. STONEY ◽  
IAN K. McNIECE
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Cd34 Cells ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

scholarly journals Extensive Ex Vivo Expansion of Functional Human Erythroid Precursors Established From Umbilical Cord Blood Cells by Defined Factors

2014 ◽  
Vol 22 (2) ◽  
pp. 451-463 ◽  
Author(s):  
Xiaosong Huang ◽  
Siddharth Shah ◽  
Jing Wang ◽  
Zhaohui Ye ◽  
Sarah N Dowey ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Erythroid Precursors ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

scholarly journals Noninvasive Bioluminescent Imaging Demonstrates Long-Term Multilineage Engraftment of Ex Vivo-Expanded CD34-Selected Umbilical Cord Blood Cells

Stem Cells ◽  
2009 ◽  
Vol 27 (8) ◽  
pp. 1932-1940 ◽  
Author(s):  
David Steiner ◽  
Juri Gelovani ◽  
Barbara Savoldo ◽  
Simon N. Robinson ◽  
William K. Decker ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Bioluminescent Imaging ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood

Acta Naturae ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 6-16 ◽  
Author(s):  
E. V. Sotnezova ◽  
E. R. Andreeva ◽  
A. I. Grigoriev ◽  
L. B. Buravkova
Keyword(s):  
Cord Blood ◽  
Progenitor Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
Cord Blood Cells

Transplantation of umbilical cord blood cells is currently widely used in modern cell therapy. However, the limited number of hematopoietic stem and progenitor cells (HSPCs) and prolonged time of recovery after the transplantation are significant limitations in the use of cord blood. Ex vivo expansion with various cytokine combinations is one of the most common approaches for increasing the number of HSPCs from one cord blood unit. In addition, there are protocols that enable ex vivo amplification of cord blood cells based on native hematopoietic microenvironmental cues, including stromal components and the tissue-relevant oxygen level. The newest techniques for ex vivo expansion of HSPCs are based on data from the elucidation of the molecular mechanisms governing the hematopoietic niche function. Application of these methods has provided an improvement of several important clinical outcomes. Alternative methods of cord blood transplantation enhancement based on optimization of HPSC homing and engraftment in patient tissues have also been successful. The goal of the present review is to analyze recent methodological approaches to cord blood HSPC ex vivo amplification.

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