Effect of a Solution with High Fluoride Concentration on Remineralization of Shallow and Deep Root Surface Caries in vitro

2001 ◽  
Vol 35 (5) ◽  
pp. 317-324 ◽  
Author(s):  
Y. Mukai ◽  
M.D. Lagerweij ◽  
J.M. ten Cate
1988 ◽  
Vol 67 (10) ◽  
pp. 1338-1341 ◽  
Author(s):  
R.L. Ettinger ◽  
D. Manderson ◽  
J.S. Wefel ◽  
M.E. Jensen

Teeth prepared as overdenture abutments are susceptible to caries, and it has been shown that brushing by itself is not sufficient to prevent this process. This study evaluated the preventive effect of a remineralization gel which has a low fluoride concentration and compared its effects with those of a phosphate fluoride gel (Karigel), which has a much higher concentration of fluoride. Twenty extracted anterior teeth from patients aged 50 to 70 years were prepared as for overdenture abutments. Each tooth was sectioned into three fragments. An acidified gel system was used to produce artificial caries lesions on the occlusal and root surfaces of each fragment. One fragment of each tooth was treated with the remineralizing gel, the second fragment with a high-fluoride gel, and the third fragment served as the control. Ten teeth were removed at two weeks and again at four weeks, and were sectioned and prepared for histological examination. The depth of the lesions was measured from standardized photomicrographs by means of a sonic digitizer. The conclusions were: (1) Lesions on the occlusal tended to be deeper than those on the root surfaces at four weeks but not at two weeks; and (2) the high-fluoride gel was more protective than the low-fluoride remineralizing solution at both time periods on the occlusal but not on the root surface.


2020 ◽  
Vol Volume 15 ◽  
pp. 7469-7479
Author(s):  
Aila Maria Cipriano Leal ◽  
Marcus Vinícius Beserra dos Santos ◽  
Edson Cavalcanti da Silva Filho ◽  
André Luis Menezes Carvalho ◽  
Cinthia Pereira Machado Tabchoury ◽  
...  

1992 ◽  
Vol 71 (3_suppl) ◽  
pp. 913-919 ◽  
Author(s):  
J.R. Mellberg

Hard-tissue substrates include primarily human and bovine enamel and human dentin. They have been used for in situ studies in a natural or sound condition, as well as flattened or containing an in vitro-formed caries-like lesion. Human enamel and dentin are generally the substrates of choice for studies of coronal and root-surface caries, respectively, but bovine enamel appears to offer a suitable alternative for many studies of enamel caries. Substrates with caries-like lesions will respond more rapidly to changes in the intra-oral mineral equilibrium and will allow both demineralization and remineralization to be determined. Findings from some studies suggest that caries-like lesions may respond somewhat differently, depending upon the degree of mineralization of the surface layer. Because in vitro findings with dentin show it to be significantly more soluble in acid than enamel, results from a study that used dentin may not be directly applicable to enamel. Both enamel and dentin substrates can be used in thin-section models. Hard-tissue substrates can also differ, depending upon their intra-oral location. Locations that result in the accumulation of plaque will behave differently from those that are plaque-free. So that plaque would accumulate, substrates have been placed approximally, beneath a fabric or steel mesh, in a protected trough, beneath a metal band or within a depression on the buccal surface. For studies requiring a determination of both demineralization and remineralization, human enamel or dentin containing a surface-softened caries-like lesion and covered with a uniform natural plaque are the substrates of choice.


2021 ◽  
Vol 18 (1) ◽  
pp. 107-112
Author(s):  
Thirumala Mothe ◽  
Patnam Umashankar ◽  
Vishnuvardhan Reddy Sultanpuram

Consuming of water contaminated with high fluoride concentration for a very long time causes health problems such as, dental and skeletal fluorosis. Hence, defluoridation of water is essentially required before consumption, when water is contaminated with high fluoride concentration. In this present study, research was focussed on to isolate bacteria which are showing fluoride resistantance from samples of ground water from high fluoride affected regions of Nalgonda. After analysis of 10 samples from different areas of Nalgonda district, two samples of ground water from Narketpally and Nampally showed the high fluoride concentrations of 9.18 ppm and 7.55 ppm respectively. Hence, in the present study, Narketpally ground water sample with highest ppm was considered to isolate fluoride resistant bacteria. A total of eight fluoride resistant organisms were purified from this sample with varying fluoride resistance on Luria Bertani agar with varying fluoride concentraion from 25mg/L to 600mg/L at pH 7. Among the eight strains isolated, three strains MB1, F and G were showing high fluoride resistance (up to 500mg/L), which were further explored for their role in bioremediation of fluoride. In batch mode study, MB1 strain showed high fluoride degradation of 68%, whereas, F and G strains showed 57% and 44%fluoride removal, respectively, when fluoride concentration was present at 20 mgL-1 at 30 °C temperature and pH 7, with dextrose (10 g) utilised as source of carbon per 100 mL media after incubation of 8 days. Results indicate that, MB1 possibly a potential fluoride resistant bacterium with high fluoride bioremediation capacity.


1989 ◽  
Vol 3 (2) ◽  
pp. 203-210 ◽  
Author(s):  
T. Yanagisawa ◽  
S. Takuma ◽  
O. Fejerskov

Materials used in this work were 13 permanent molars exhibiting dental fluorosis (between 5 and 9 on the Thylstrup-Fejerskov scale, 1978) obtained from adults (aged 20-40 years) living in regions with 3.5 ppm fluoride in the water supplies. Small but deep occlusal caries lesions necessitated extraction. Light and polarized microscopic, microradiographic, electron microscopic, and electron-probe- and ion-micro-analytical studies were made. Enamel surfaces were generally cloudy to opaque, with several pits or defects of various sizes and degrees of brown-staining. An extensively hypomineralized area extended from the inner enamel to the surface layer, which was mineralized to a high degree. The hypomineralized area contained sparsely arranged, flattened, hexagonal crystals with either perforated centers or defects extending from the perimeter and indicating either no or low fluoride content. The highly mineralized surface layer, however, was composed of many large, flattened, hexagonal crystals and extremely small, irregularly shaped crystals. Both types were free of central perforations and defects. A high fluoride concentration was determined in the highly mineralized surface layer. These findings suggest that the hypomineralized area undergoes caries-like changes in terms of crystal dissolution and that the highly mineralized surface layer contains hydroxyapatite and fluoridated-hydroxyapatite, or fluorapatite, or both.


2006 ◽  
Vol 40 (2) ◽  
pp. 148-153 ◽  
Author(s):  
M.D. Lagerweij ◽  
W. Buchalla ◽  
S. Kohnke ◽  
K. Becker ◽  
Á.M. Lennon ◽  
...  

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