Low-Protein Diet Suppresses Serum Insulin-Like Growth Factor-1 and Decelerates the Progression of Growth Hormone-Induced Glomerulosclerosis

2001 ◽  
Vol 21 (4) ◽  
pp. 331-339 ◽  
Author(s):  
Sonia Q. Doi ◽  
Suvi Rasaiah ◽  
Ivan Tack ◽  
Jagannatha Mysore ◽  
John J. Kopchick ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Serum Insulin ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Insulin Like Growth Factor ◽  
Low Protein

scholarly journals Insulin-like growth factor-I, but not growth hormone, is dependent on a high protein intake to increase nitrogen balance in the rat

1999 ◽  
Vol 81 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Myriam Sanchez-Gomez ◽  
Kjell Malmlöf ◽  
Wilson Mejia ◽  
Antonio Bermudez ◽  
Maria Teresa Ochoa ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Dietary Protein ◽  
Protein Diet ◽  
N Balance ◽  
Low Protein Diet ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Low Protein ◽  
Igf I

The aim of the present study was to investigate the influence of dietary protein level on the protein anabolic effects of growth hormone (GH) and insulin-like growth factor-I (IGF-I). Female growing rats were fed on either a high- or a low-protein diet with crude protein contents of 222 and 83 g/kg respectively. The diets contained the same amount of metabolizable energy (15·1 MJ/kg) and were given during a 14 d period. During the same time, three groups of rats (n 8) on each diet received subcutaneous infusions of either saline, recombinant human GH (rhGH) or recombinant human IGF-I (rhIGF-I). rhGH and rhIGF-I were given in doses of 360 and 500 μg/d respectively. The low-protein diet alone reduced significantly (P < 0·05) IGF-I concentrations in serum and in tissue taken from the gastrocnemius muscle as well as IGF-I mRNA from the same muscle. The responses to rhGH and rhIGF-I in terms of muscle IGF-I and its mRNA were variable. However, when rhIGF-I was infused into rats on the high-protein diet, significantly elevated levels of IGF-I in muscle tissues could be observed. This was associated with a significantly (P < 0·05) increased N balance, whereas rhGH significantly (P < 0·05) enhanced the N balance in rats on the low-protein diet. Thus, it can be concluded that the level of dietary protein ingested regulates not only the effect of IGF-I on whole-body N economy but also the regulation of IGF-I gene expression in muscles. The exact mechanism by which GH exerts its protein anabolic effect, however, remains to be elucidated.

Low-Protein Diet Regulates a Proximal Nephron Insulin-like Growth Factor Binding Protein

1994 ◽  
Vol 23 (6) ◽  
pp. 849-855 ◽  
Author(s):  
Michael K. Hise ◽  
Nicki M. Mantzouris ◽  
Joel S. Lahn ◽  
M. Saeed Sheikh ◽  
Zhi-Ming Shao ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Low Protein

scholarly journals Insulin-like growth factor-1 and its binding proteins during a low-protein diet in chronic renal failure.

1995 ◽  
Vol 6 (5) ◽  
pp. 1427-1433
Author(s):  
D Fouque ◽  
Y Le Bouc ◽  
M Laville ◽  
F Combarnous ◽  
M O Joly ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Growth Factor ◽  
Protein Intake ◽  
Binding Proteins ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Insulin Like Growth Factor ◽  
Low Protein ◽  
Keto Acids

The effects of a low-protein diet on the serum insulin-like growth factor (IGF)-1 and IGF binding proteins (IGFBP) were investigated during a 3-month controlled study in 12 adult chronic renal failure patients. Six patients were randomly supplemented with keto acids (Cetolog, Clintec, Velizy, France). Protein intake was prescribed so that both groups were isonitrogenous. Dietary survey included a monthly 3-day food record and a 24-h urinary urea measurement. After a 4- to 6-wk equilibrium period (1.11 g of protein, 32 kcal/kg body wt per day), patients reduced their protein intake to 0.71 g protein/kg body wt per day. Energy intake was kept constant (31 kcal/kg body wt per day) during the 3-month period. Serum IGF-1 levels were in normal range and, for 11 of the 12 patients, were correlated with the GFR (P = 0.01). These serum IGF-1 values did not decrease after reducing the protein intake. By Western ligand blotting, serum IGFBP1, IGFBP2, and IGFBP4 levels were significantly higher than normal adults, whereas the IGFBP3 level was not increased. IGFBP were not modified when protein intake was reduced. The IGFBP1 level was elevated despite a normal insulin level. IGFBP4 changes were inversely correlated with IGF-1 variations. There was no difference between groups receiving or not receiving the keto acids. Thus, in adult chronic renal failure, reducing protein intake by 40% did not modify the growth hormone/IGF-1/IGFBP axis.

scholarly journals Physiological concentration of amino acids regulates insulin-like-growth-factor-binding protein 1 expression

1998 ◽  
Vol 334 (1) ◽  
pp. 147-153 ◽  
Author(s):  
Céline JOUSSE ◽  
Alain BRUHAT ◽  
Marc FERRARA ◽  
Pierre FAFOURNOUX
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Growth Factor ◽  
Hepg2 Cells ◽  
Binding Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Insulin Like Growth Factor ◽  
Low Protein ◽  
Igf I

Protein undernutrition is characterized by growth failure in young growing animals. Current evidence suggests that biosynthesis of insulin-like growth factor (IGF)-I and IGF-binding protein 1 (IGFBP-1) are key control points for nutritional regulation of growth. Here we examined the role of amino acid limitation in regulating the IGFBP-1 expression in the hepatic cell line. Our data show that leucine limitation strongly induces IGFBP-1 without affecting IGF-I and IGF-II expression in human HepG2 cells and in isolated rat hepatocytes. Depletion of arginine, cystine and all essential amino acids leads to induction of IGFBP-1 mRNA and protein expression in a dose-dependent manner. IGFBP-1 expression is significantly induced by leucine concentration in the range of that observed in the blood of rats fed a low-protein diet or in humans affected by kwashiorkor. Moreover, treatment of HepG2 cells with amino acids at a concentration reproducing the amino acid concentration found in portal blood of rats fed a low-protein diet leads to a significantly higher expression of IGFBP-1. These data represent the first demonstration that an amino acid limitation, as occurs during dietary protein deficiency, induces IGFBP-1 expression in hepatic cells. Therefore, amino acids by themselves can play, in concert with hormones, an important role in the control of gene expression.

Administration of growth hormone to pigs alters the relative amount of insulin-like growth factor-I mRNA in liver and skeletal muscle

1991 ◽  
Vol 130 (3) ◽  
pp. 331-NP ◽  
Author(s):  
A. L. Grant ◽  
W. G. Helfericht ◽  
S. A. Kramer ◽  
R. A. Merkel ◽  
W. G. Bergen
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Low Protein ◽  
Igf I

ABSTRACT The relative amount of insulin-like growth factor-I (IGF-I) mRNA was determined in the liver and skeletal muscle of market weight crossbred barrows (castrated male pigs) using a solution hybridization–nuclease protection assay. Pigs were given either 50 μg recombinant porcine GH per kg body weight or vehicle daily for 24 days i.m. They were fed corn–soybean meal diets containing either 140 or 200 g crude protein/kg (low or high protein). The percentage of muscle in the carcasses of pigs given GH was greater (P <0·01) than that of controls. Relative to controls, GH increased (P <0·05) the amount of liver IGF-I mRNA by 2·7-fold in pigs fed the low protein diet and 3·0-fold in pigs fed the high protein diet. The amount of IGF-I mRNA in the muscles of GH-treated pigs was 77% and 84% of control pigs in those fed the low and high protein diets respectively (P <0·08). GH increased (P <0·001) the serum concentration of IGF-I 1·6-fold in pigs fed the low protein diet and 2·0-fold in those fed the high protein diet. These results indicate that the administration of GH to pigs influences the relative amount of liver IGF-I mRNA. The increased amount of liver IGF-I mRNA and the increased serum IGF-I concentrations suggest that IGF-I plays an endocrine role in mediating GH-induced muscle hypertrophy in pigs. Journal of Endocrinology (1991) 130, 331–338

Sign in / Sign up

Export Citation Format

Share Document