Decreased Bilirubin-Binding Capacity in Uremic Serum Caused by an Accumulation of Furan Dicarboxylic Acid

Nephron ◽  
2000 ◽  
Vol 85 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Yasuhiro Tsutsumi ◽  
Toru Maruyama ◽  
Akira Takadate ◽  
Hidekata Shimada ◽  
Masaki Otagiri
Keyword(s):  
Dicarboxylic Acid ◽  
Binding Capacity ◽  
Uremic Serum ◽  
Bilirubin Binding

Unbound Bilirubin and Kernicterus in Low-Birth-Weight Infants

PEDIATRICS ◽  
1982 ◽  
Vol 69 (4) ◽  
pp. 481-485 ◽  
Author(s):  
William J. Cashore ◽  
William Oh
Keyword(s):  
Preterm Infants ◽  
Binding Capacity ◽  
Assisted Ventilation ◽  
Albumin Concentration ◽  
Low Birth Weight Infants ◽  
Indirect Bilirubin ◽  
Clinical Complications ◽  
Horseradish Peroxidase Method ◽  
Unbound Bilirubin ◽  
Bilirubin Binding

Unbound bilirubin, bilirubin binding capacity, and bilirubin binding affinity were determined by the horseradish peroxidase method at the time of maximum hyperbilirubinemia and/or before exchange transfusions in 13 preterm infants who later died and had autopsies performed. Five of the 13 infants had kernicterus at autopsy. There were no significant differences in weight, gestational age, highest indirect bilirubin level, albumin concentration, severity of acidosis, use of assisted ventilation, sepsis, or other major clinical complications between the five infants with kernicterus and the eight infants without kernicterus. Compared with the eight nonkernicteric infants, the five kernicteric infants had significantly higher unbound bilirubin concentrations (13 ± 10 vs 27 ± 9 nmoles/liter, respectively, P < .05) and significantly lower bilirubin binding capacity and affinity. The data suggest an association between low bilirubin binding capacity and affinity, increased unbound bilirubin, and kernicterus in preterm infants with severe clinical complications.

A multi-center evaluation of a device for measurement of bilirubin binding capacity in neonates: the effects of gestational age, Intralipid exposure and illness severity

2019 ◽  
Vol 39 (6) ◽  
pp. 883-888
Author(s):  
David L. Schutzman ◽  
Vinod K. Bhutani ◽  
Martin E Castillo Cuadrado ◽  
Angelo A Lamola ◽  
Ivan Frantz ◽  
...  
Keyword(s):  
Gestational Age ◽  
Binding Capacity ◽  
Illness Severity ◽  
Bilirubin Binding

Faster estimation of reserve bilirubin binding capacity of serum from the neonate by thin-layer chromatography on sephadex.

1977 ◽  
Vol 23 (3) ◽  
pp. 541-545 ◽  
Author(s):  
F Trivin ◽  
M Odièvre ◽  
A Lemonnier
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Binding Capacity ◽  
Gel Chromatography ◽  
Standard Solution ◽  
Bilirubin Binding ◽  
Layer Chromatography

Abstract Knowledge of the reserve bilirubin-binding capacity is useful in management of hyperbilirubinemia in the newborn. We describe thin-layer gel chromatography that permits measurement of this variable, with a 250-mul sample of serum, within 50 min. Single samples from four different infants can be analyzed on the same plate. The capacity of 40 sera from jaundiced and of 19 sera from nonjaundiced infants was measured. Results by thin-layer Sephadex gel chromatography and column Sephadex chromatography correlated well. Dithiothreitol, added to the standard bilirubin solution, protected bilirubin from oxidation for at least six days, which obviated the time consuming preparation of standard solution before such determination.

Fluorescent dye method for determination of the bilirubin-binding capacity of serum albumin

1975 ◽  
Vol 86 (2) ◽  
pp. 280-285 ◽  
Author(s):  
Kwang-sun Lee ◽  
Lawrence M. Gartner ◽  
Ilana Zarafu
Keyword(s):  
Serum Albumin ◽  
Binding Capacity ◽  
Fluorescent Dye ◽  
Bilirubin Binding
Sign in / Sign up

Export Citation Format

Share Document