Effects of Diisopropylamine Dichloroacetate on Proliferation and Differentiation of Normal Human Keratinocytes in vitro

1999 ◽  
Vol 12 (6) ◽  
pp. 317-325 ◽  
Author(s):  
Nobuo Kitamura ◽  
Yukiko Ota ◽  
Kunio Mimura
Keyword(s):  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Proliferation And Differentiation ◽  
Normal Human

scholarly journals Expression of C4.4A in an In Vitro Human Tissue-Engineered Skin Model

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Benedikte Jacobsen ◽  
Danielle Larouche ◽  
Olivier Rochette-Drouin ◽  
Michael Ploug ◽  
Lucie Germain
Keyword(s):  
Self Assembly ◽  
Human Keratinocytes ◽  
Skin Substitutes ◽  
Pathological Conditions ◽  
Monolayer Cultures ◽  
Normal Human Keratinocytes ◽  
Stratum Spinosum ◽  
Normal Human ◽  
Suprabasal Keratinocytes

A multi-LU-domain-containing protein denoted C4.4A exhibits a tightly regulated membrane-associated expression in the suprabasal layers of stratified squamous epithelia such as skin and the esophagus, and the expression of C4.4A is dysregulated in various pathological conditions. However, the biological function of C4.4A remains unknown. To enable further studies, we evaluated the expression of C4.4A in monolayer cultures of normal human keratinocytes and in tissue-engineered skin substitutes (TESs) produced by the self-assembly approach, which allow the formation of a fully differentiated epidermis tissue. Results showed that, in monolayer, C4.4A was highly expressed in the centre of keratinocyte colonies at cell-cell contacts areas, while some cells located at the periphery presented little C4.4A expression. In TES, emergence of C4.4A expression coincided with the formation of the stratum spinosum. After the creation of a wound within the TES, C4.4A expression was observed in the suprabasal keratinocytes of the migrating epithelium, with the exception of the foremost leading keratinocytes, which were negative for C4.4A. Our results are consistent with previous data in mouse embryogenesis and wound healing. Based on these findings, we conclude that this human TES model provides an excellent surrogate for studies of C4.4A and Haldisin expressions in human stratified epithelia.

scholarly journals Ascorbic Acid, Ultraviolet C Rays, and Glucose but not Hyperthermia Are Elicitors of Humanβ-Defensin 1 mRNA in Normal Keratinocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Luis Antonio Cruz Díaz ◽  
María Guadalupe Flores Miramontes ◽  
Paulina Chávez Hurtado ◽  
Kirk Allen ◽  
Marisela Gonzalez Ávila ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ascorbic Acid ◽  
Human Keratinocytes ◽  
Independent Manner ◽  
Innate Defense ◽  
Normal Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Ultraviolet C ◽  
Normal Human

Hosts’ innate defense systems are upregulated by antimicrobial peptide elicitors (APEs). Our aim was to investigate the effects of hyperthermia, ultraviolet A rays (UVA), and ultraviolet C rays (UVC) as well as glucose and ascorbic acid (AA) on the regulation of humanβ-defensin 1 (DEFB1), cathelicidin (CAMP), and interferon-γ(IFNG) genes in normal human keratinocytes (NHK). The indirectin vitroantimicrobial activity againstStaphylococcus aureusandListeria monocytogenesof these potential APEs was tested. We found that AA is a more potent APE forDEFB1than glucose in NHK. Glucose but not AA is an APE forCAMP. Mild hypo- (35°C) and hyperthermia (39°C) are not APEs in NHK. AA-dependentDEFB1upregulation below 20 mM predictsin vitroantimicrobial activity as well as glucose- and AA-dependentCAMPandIFNGupregulation. UVC upregulatesCAMPandDEFB1genes but UVA only upregulates theDEFB1gene. UVC is a previously unrecognized APE in human cells. Our results suggest that glucose upregulatesCAMPin an IFN-γ-independent manner. AA is an elicitor of innate immunity that will challenge the current concept of late activation of adaptive immunity of this vitamin. These results could be useful in designing new potential drugs and devices to combat skin infections.

Lack of evidence for TARC/CCL17 production by normal human keratinocytes in vitro

2003 ◽  
Vol 31 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Teruko Tsuda ◽  
Mikiko Tohyama ◽  
Kenshi Yamasaki ◽  
Yuji Shirakata ◽  
Yoko Yahata ◽  
...  
Keyword(s):  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Normal Human

Tranilast inhibits the cell growth of normal human keratinocytes in vitro

2012 ◽  
Vol 304 (9) ◽  
pp. 745-753 ◽  
Author(s):  
Miyoko Kubo ◽  
Ying Zhao ◽  
Takahiko Moriguchi
Keyword(s):  
Cell Growth ◽  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Normal Human
Sign in / Sign up

Export Citation Format

Share Document