Use of Diffusion-Weighted MRI and Neurological Deficit Scores to Demonstrate Beneficial Effects of Isradipine in a Rat Model of Focal Ischemia

Pharmacology ◽  
1999 ◽  
Vol 58 (6) ◽  
pp. 292-299 ◽  
Author(s):  
Sudeep Chandra ◽  
Ray F. White ◽  
Daryl Everding ◽  
Giora Z. Feuerstein ◽  
Robert W. Coatney ◽  
...  
Keyword(s):  
Rat Model ◽  
Neurological Deficit ◽  
Focal Ischemia ◽  
Diffusion Weighted Mri ◽  
Beneficial Effects ◽  
Diffusion Weighted

scholarly journals Response Monitoring with [18F]FLT PET and Diffusion-Weighted MRI After Cytotoxic 5-FU Treatment in an Experimental Rat Model for Colorectal Liver Metastases

2016 ◽  
Vol 19 (4) ◽  
pp. 540-549 ◽  
Author(s):  
Sandra Heskamp ◽  
Linda Heijmen ◽  
Danny Gerrits ◽  
Janneke D. M. Molkenboer-Kuenen ◽  
Edwin G. W. ter Voert ◽  
...  
Keyword(s):  
Liver Metastases ◽  
Rat Model ◽  
Colorectal Liver Metastases ◽  
Response Monitoring ◽  
Diffusion Weighted Mri ◽  
Flt Pet ◽  
Diffusion Weighted ◽  
Experimental Rat Model ◽  
And Diffusion

scholarly journals The KCa3.1 Blocker TRAM-34 Reduces Infarction and Neurological Deficit in a Rat Model of Ischemia/Reperfusion Stroke

2011 ◽  
Vol 31 (12) ◽  
pp. 2363-2374 ◽  
Author(s):  
Yi-Je Chen ◽  
Girija Raman ◽  
Silke Bodendiek ◽  
Martha E O'Donnell ◽  
Heike Wulff
Keyword(s):  
Ischemic Stroke ◽  
Rat Model ◽  
Neurological Deficit ◽  
Ischemia Reperfusion ◽  
Artery Occlusion ◽  
Beneficial Effects ◽  
Inflammatory Damage ◽  
Male Wistar Rats ◽  
Cerebral Artery Occlusion ◽  
The Brain

Microglia and brain infiltrating macrophages significantly contribute to the secondary inflammatory damage in the wake of ischemic stroke. Here, we investigated whether inhibition of KCa3.1 (IKCa1/KCNN4), a calcium-activated K+ channel that is involved in microglia and macrophage activation and expression of which increases on microglia in the infarcted area, has beneficial effects in a rat model of ischemic stroke. Using an HPLC/MS assay, we first confirmed that our small molecule KCa3.1 blocker TRAM-34 effectively penetrates into the brain and achieves micromolar plasma and brain concentrations after intraperitoneal injection. Then, we subjected male Wistar rats to 90 minutes of middle cerebral artery occlusion (MCAO) and administered either vehicle or TRAM-34 (10 or 40 mg/kg intraperitoneally twice daily) for 7 days starting 12 hours after reperfusion. Both compound doses reduced infarct area by ∼50% as determined by hematoxylin & eosin staining on day 7 and the higher dose also significantly improved neurological deficit. We further observed a significant reduction in ED1+-activated microglia and TUNEL-positive neurons as well as increases in NeuN+ neurons in the infarcted hemisphere. Our findings suggest that KCa3.1 blockade constitutes an attractive approach for the treatment of ischemic stroke because it is still effective when initiated 12 hours after the insult.

Diffusion-weighted MRI does not reflect kidney fibrosis in a rat model of fibrosis

2015 ◽  
Vol 42 (4) ◽  
pp. 990-998 ◽  
Author(s):  
Peter Boor ◽  
Michael Perkuhn ◽  
Martin Weibrecht ◽  
Stephanie Zok ◽  
Ina V. Martin ◽  
...  
Keyword(s):  
Rat Model ◽  
Diffusion Weighted Mri ◽  
Kidney Fibrosis ◽  
Diffusion Weighted

Comparison of diffusion-weighted MRI with changes in cell volume in a rat model of brain injury

1994 ◽  
Vol 7 (1-2) ◽  
pp. 96-100 ◽  
Author(s):  
H. B. Verheul ◽  
R. Balazs ◽  
J. W. Berkelbach van der Sprenkel ◽  
C. A. F. Tulleken ◽  
K. Nicolay ◽  
...  
Keyword(s):  
Brain Injury ◽  
Cell Volume ◽  
Rat Model ◽  
Diffusion Weighted Mri ◽  
Diffusion Weighted

scholarly journals The Effects of Delayed Reduction of Tonic Inhibition on Ischemic Lesion and Sensorimotor Function

2015 ◽  
Vol 35 (10) ◽  
pp. 1601-1609 ◽  
Author(s):  
Evelyn MR Lake ◽  
Joydeep Chaudhuri ◽  
Lynsie Thomason ◽  
Rafal Janik ◽  
Milan Ganguly ◽  
...  
Keyword(s):  
Rat Model ◽  
Focal Ischemia ◽  
Stroke Recovery ◽  
Gamma Aminobutyric Acid ◽  
Ischemic Lesion ◽  
Chronic Stage ◽  
Beneficial Effects ◽  
Sensorimotor Deficits ◽  
Skilled Reaching ◽  
Better Than

To aid in development of chronic stage treatments for sensorimotor deficits induced by ischemic stroke, we investigated the effects of GABA antagonism on brain structure and fine skilled reaching in a rat model of focal ischemia induced via cortical microinjections of endothelin-1 (ET-1). Beginning 7 days after stroke, animals were administered a gamma-aminobutyric acid (GABAA) inverse agonist, L-655,708, at a dose low enough to afford α5-GABAA receptor specificity. A week after stroke, the ischemic lesion comprised a small hypointense necrotic core (6 ± 1 mm3) surrounded by a large (62 ± 11 mm3) hyperintense perilesional region; the skilled reaching ability on the Montoya staircase test was decreased to 34% ± 2% of the animals' prestroke performance level. On L-655,708 treatment, animals showed a progressive decrease in total stroke volume (13 ± 4 mm3 per week), with no change in animals receiving placebo. Concomitantly, treated animals' skilled reaching progressively improved by 9% ± 1% per week, so that after 2 weeks of treatment, these animals performed at 65% ± 6% of their baseline ability, which was 25% ± 11% better than animals given placebo. These data indicate beneficial effects of delayed, sustained low-dose GABAA antagonism on neuroanatomic injury and skilled reaching in the chronic stage of stroke recovery in an ET-1 rat model of focal ischemia.

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