Microdissection of Tissue Sections: Application to the Molecular Genetic Characterisation of Premalignant Lesions

Pathobiology ◽  
2000 ◽  
Vol 68 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Axel Walch ◽  
Paul Komminoth ◽  
Peter Hutzler ◽  
Michaela Aubele ◽  
Heinz Höfler ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Premalignant Lesions ◽  
Genetic Characterisation ◽  
Tissue Sections

Molecular genetic characterisation and expression profiling of calpain 3 transcripts in red sea bream (Pagrus major)

2020 ◽  
Vol 98 ◽  
pp. 19-24
Author(s):  
Seong Don Hwang ◽  
Kwang-Min Choi ◽  
Jee Youn Hwang ◽  
Mun-Gyeong Kwon ◽  
Ji-Min Jeong ◽  
...  
Keyword(s):  
Red Sea ◽  
Expression Profiling ◽  
Molecular Genetic ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Pagrus Major ◽  
Genetic Characterisation ◽  
Calpain 3

Molecular Genetic Characterisation of Frontotemporal Dementia on Chromosome 3

1999 ◽  
Vol 10 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Anthony Ashworth ◽  
Sarah Lloyd ◽  
Jeremy Brown ◽  
Susanne Gydesen ◽  
S. Asger Sorensen ◽  
...  
Keyword(s):  
Frontotemporal Dementia ◽  
Molecular Genetic ◽  
Chromosome 3 ◽  
Genetic Characterisation

scholarly journals Integrin αvβ6 as a Target for Tumor-Specific Imaging of Vulvar Squamous Cell Carcinoma and Adjacent Premalignant Lesions

Cancers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 6006
Author(s):  
Bertine W. Huisman ◽  
Merve Cankat ◽  
Tjalling Bosse ◽  
Alexander L. Vahrmeijer ◽  
Robert Rissmann ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Near Infrared ◽  
Premalignant Lesions ◽  
Vulvar Intraepithelial Neoplasia ◽  
Surgical Removal ◽  
Vulvar Squamous Cell Carcinoma ◽  
Tissue Sections ◽  
Integrin Αvβ6

Surgical removal of vulvar squamous cell carcinoma (VSCC) is associated with significant morbidity and high recurrence rates. This is at least partially related to the limited visual ability to distinguish (pre)malignant from normal vulvar tissue. Illumination of neoplastic tissue based on fluorescent tracers, known as fluorescence-guided surgery (FGS), could help resect involved tissue and decrease ancillary mutilation. To evaluate potential targets for FGS in VSCC, immunohistochemistry was performed on paraffin-embedded premalignant (high grade squamous intraepithelial lesion and differentiated vulvar intraepithelial neoplasia) and VSCC (human papillomavirus (HPV)-dependent and -independent) tissue sections with healthy vulvar skin as controls. Sections were stained for integrin αvβ6, CAIX, CD44v6, EGFR, EpCAM, FRα, MRP1, MUC1 and uPAR. The expression of each marker was quantified using digital image analysis. H-scores were calculated and percentages positive cells, expression pattern, and biomarker localization were assessed. In addition, tumor-to-background ratios were established, which were highest for (pre)malignant vulvar tissues stained for integrin αvβ6. In conclusion, integrin αvβ6 allowed for the most robust discrimination of VSCCs and adjacent premalignant lesions compared to surrounding healthy tissue in immunohistochemically stained tissue sections. The use of an αvβ6 targeted near-infrared fluorescent probe for FGS of vulvar (pre)malignancies should be evaluated in future studies.

Molecular genetic characterisation of intracerebrally transplanted brain tumours

1999 ◽  
Vol 51 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Jürgen Schlegel ◽  
Guido Piontek ◽  
Christian Kühne ◽  
Hans-Jürgen Bartels ◽  
Alison Kraus ◽  
...  
Keyword(s):  
Brain Tumours ◽  
Molecular Genetic ◽  
Genetic Characterisation
Sign in / Sign up

Export Citation Format

Share Document