Intracellular Application of Calmidazolium Increases Ca2+ Current through Activation of Protein Kinase A in Cultured Vascular Smooth Muscle Cells

1998 ◽  
Vol 35 (5) ◽  
pp. 303-309 ◽  
Author(s):  
Masanori Sunagawa ◽  
Hisashi Yokoshiki ◽  
Takashi Seki ◽  
Nicholas Sperelakis
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Protein Kinase A ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Kinase A

scholarly journals Activation of Inward Rectifier K+ Channel 2.1 by PDGF-BB in Rat Vascular Smooth Muscle Cells through Protein Kinase A

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Chengchun Tang ◽  
Dong Wang ◽  
Erfei Luo ◽  
Gaoliang Yan ◽  
Bo Liu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Protein Kinase A ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Inward Rectifier ◽  
Kinase A ◽  
Stimulation Of

Platelet-derived growth factor-BB (PDGF-BB) can induce the proliferation, migration, and phenotypic modulation of vascular smooth muscle cells (VSMCs). We used patch clamp methods to study the effects of PDGF-BB on inward rectifier K+ channel 2.1 (Kir2.1) channels in rat thoracic aorta VSMCs (RASMCs). PDGF-BB (25 ng/mL) increased Kir2.x currents (−11.81±2.47 pA/pF, P<0.05 vs. CON, n=10). Ba2+(50 μM) decreased Kir2.x currents (−2.13±0.23 pA/pF, P<0.05 vs. CON, n=10), which were promoted by PDGF-BB (−6.98±1.03 pA/pF). PDGF-BB specifically activates Kir2.1 but not Kir2.2 and Kir2.3 channels in HEK-293 cells. The PDGF-BB-induced stimulation of Kir2.1 currents was blocked by the PDGF-BB receptor β (PDGF-BBRβ) inhibitor AG1295 and was not affected by the PDGF-BBRα inhibitor AG1296. The PDGF-BB-induced stimulation of Kir2.1 currents was blocked by the protein kinase A inhibitor Rp-8-CPT-cAMPs; however, the antagonist of protein kinase B (GSK690693) had marginal effects on current activity. The PDGF-BB-induced stimulation of Kir2.1 currents was enhanced by forskolin, an adenylyl cyclase (AC) activator, and was blocked by the AC inhibitor SQ22536. We conclude that PDGF-BB increases Kir2.1 currents via PDGF-BBRβ through activation of cAMP-PKA signaling in RASMCs.

scholarly journals Protein Kinase A-regulated Assembly of a MEF2·HDAC4 Repressor Complex Controls c-Jun Expression in Vascular Smooth Muscle Cells

2009 ◽  
Vol 284 (28) ◽  
pp. 19027-19042 ◽  
Author(s):  
Joseph W. Gordon ◽  
Christina Pagiatakis ◽  
Jahan Salma ◽  
Min Du ◽  
John J. Andreucci ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Protein Kinase A ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Repressor Complex ◽  
Kinase A
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