Superantigen-Induced T Cell Death by Apoptosis: Analysis on a Single Cell Level and Effect of IFN-γ and IL-4 Treatment

2000 ◽  
Vol 121 (3) ◽  
pp. 215-223 ◽  
Author(s):  
Anne K. Weber ◽  
Ulrich Wahn ◽  
Harald Renz
Keyword(s):  
Cell Death ◽  
T Cell ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Ifn Γ

Abstract 4041: Coupling neoantigen specific T cell clonotypes and their molecular phenotypes at the single cell level in resectable anti-PD-1 treated NSCLC

2019 ◽  
Author(s):  
Justina X. Caushi ◽  
Zhicheng Ji ◽  
Jiajia Zhang ◽  
Margueritta El Asmar ◽  
Valsamo Anagnostou ◽  
...  
Keyword(s):  
T Cell ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Molecular Phenotypes

scholarly journals Research Resource: Monitoring Endoplasmic Reticulum Membrane Integrity in β-Cells at the Single-Cell Level

2015 ◽  
Vol 29 (3) ◽  
pp. 473-480 ◽  
Author(s):  
Kohsuke Kanekura ◽  
Jianhong Ou ◽  
Takashi Hara ◽  
Lihua J. Zhu ◽  
Fumihiko Urano
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Er Stress ◽  
Single Cell ◽  
Membrane Integrity ◽  
Membrane Permeabilization ◽  
Single Cell Level ◽  
Cell Level ◽  
Β Cell ◽  
Er Membrane

Abstract Endoplasmic reticulum (ER) membrane integrity is an emerging target for human chronic diseases associated with ER stress. Despite the underlying importance of compromised ER membrane integrity in disease states, the entire process leading to ER membrane permeabilization and cell death is still not clear due to technical limitations. Here we describe a novel method for monitoring ER membrane integrity at the single-cell level in real time. Using a β-cell line expressing ER-targeted redox sensitive green fluorescent protein, we could identify a β-cell population undergoing ER membrane permeabilization induced by palmitate and could monitor cell fate and ER stress of these cells at the single-cell level. Our method could be used to develop a novel therapeutic modality targeting the ER membrane for ER-associated disorders, including β-cell death in diabetes, neurodegeneration, and Wolfram syndrome.

T-cell cytokine profile evaluated at the single cell level in BAL and blood in allergic asthma.

1996 ◽  
Vol 14 (4) ◽  
pp. 319-326 ◽  
Author(s):  
N Krug ◽  
J Madden ◽  
A E Redington ◽  
P Lackie ◽  
R Djukanovic ◽  
...  
Keyword(s):  
T Cell ◽  
Single Cell ◽  
Allergic Asthma ◽  
Cytokine Profile ◽  
Single Cell Level ◽  
Cell Level

Enhanced IFN-γ Production in Vitro by CD8+ T Cells in Hemophiliacs with AIDS as Demonstrated on the Single-Cell Level

1999 ◽  
Vol 92 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Y. Pae ◽  
H. Minagawa ◽  
J. Hayashi ◽  
S. Kashiwagi ◽  
Y. Yanagi
Keyword(s):  
T Cells ◽  
Single Cell ◽  
Cd8 T Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Ifn Γ

scholarly journals Single-cell RNA-Seq reveals the link between CD45 isoforms and tumor-infiltrating T cells heterogeneity in liver cancer

2020 ◽  
Author(s):  
Biaofeng Zhou ◽  
Shang Liu ◽  
Liang Wu ◽  
Yan Sun ◽  
Jie Chen ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Single Cell ◽  
Cell Function ◽  
Effector Memory ◽  
Single Cell Level ◽  
Cell Level ◽  
Cd45 Isoforms ◽  
And Function

AbstractCD45 isoforms play a major role in characterizing T cell function, phenotype, and development. However, there is lacking comprehensive interrogation about the relationship between CD45 isoforms and T lymphocytes from cancer patients at the single-cell level yet. Here, we investigated the CD45 isoforms component of published 5,063 T cells of hepatocellular carcinoma (HCC), which has been assigned functional states. We found that the distribution of CD45 isoforms in T lymphocytes cells depended on tissue resource, cell type, and functional state. Further, we demonstrated that CD45RO and CD45RA dominate in characterizing the phenotype and function of T cell though multiple CD45 isoforms coexist in T cells, through a novel alternative splicing pattern analysis. We identified a novel development trajectory of tumor-infiltrating T cells from Tcm to Temra (effector memory T cells re-expresses CD45RA) after detecting two subpopulations in state of transition, Tcm (central memory T) and Tem (effector memory T). Temra, capable of high cytotoxic characteristics, was discovered to be associated with the stage of HCC and may be a target of immunotherapy. Our study presents a comprehension of the connection between CD45 isoforms and the function, states, sources of T lymphocytes cells in HCC patients at the single-cell level, providing novel insight for the effect of CD45 isoforms on T cell heterogeneity.

T cell activation on a single-cell level in dielectrophoresis-based microfluidic devices

2008 ◽  
Vol 1202 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Michael Kirschbaum ◽  
Magnus Sebastian Jaeger ◽  
Tim Schenkel ◽  
Tanja Breinig ◽  
Andreas Meyerhans ◽  
...  
Keyword(s):  
T Cell ◽  
Single Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Microfluidic Devices ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Validation of a Combined Transcriptome and T Cell Receptor Alpha/Beta (TRA/TRB) Repertoire Assay at the Single Cell Level for Paucicellular Samples

2020 ◽  
Vol 11 ◽  
Author(s):  
Nicolle H. R. Litjens ◽  
Anton W. Langerak ◽  
Amy C. J. van der List ◽  
Mariska Klepper ◽  
Maaike de Bie ◽  
...  
Keyword(s):  
T Cell ◽  
Single Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Single Cell Level ◽  
Cell Level ◽  
Receptor Alpha ◽  
Alpha Beta ◽  
Cell Receptor Alpha

The CFSE Distribution Assay is a Powerful Technique for the Analysis of Radiation–Induced Cell Death and Survival on a Single–Cell Level

2005 ◽  
Vol 181 (7) ◽  
pp. 456-462 ◽  
Author(s):  
Franz Rödel* ◽  
Sandra Franz* ◽  
Ahmed Sheriff ◽  
Udo Gaipl ◽  
Petra Heyder ◽  
...  
Keyword(s):  
Cell Death ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Powerful Technique ◽  
Radiation Induced
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