Evidence for a Birch Pollen–Specific Cutaneous T–Cell Response in Food–Responsive Atopic Dermatitis

1999 ◽  
Vol 118 (2-4) ◽  
pp. 230-231 ◽  
Author(s):  
Thomas Werfel ◽  
Renate Reekers ◽  
Marc Busche ◽  
Petra Schmidt ◽  
Anja Constien ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
T Cell ◽  
Cell Response ◽  
Birch Pollen ◽  
T Cell Response

Heterogeneity in the Polyclonal T Cell Response to Birch Pollen Allergens

1997 ◽  
Vol 114 (3) ◽  
pp. 272-277 ◽  
Author(s):  
D. Dormann ◽  
E. Montermann ◽  
L. Klimek ◽  
B. Weber ◽  
C. Ebner ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
Birch Pollen ◽  
T Cell Response ◽  
Pollen Allergens

scholarly journals Staphylococcal enterotoxins modulate the effector CD4+ T cell response by reshaping the gene expression profile in adults with atopic dermatitis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Raquel Leao Orfali ◽  
Fabio Seiti Yamada Yoshikawa ◽  
Luanda Mara da Silva Oliveira ◽  
Natalli Zanete Pereira ◽  
Josenilson Feitosa de Lima ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Atopic Dermatitis ◽  
T Cell ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Cell Response ◽  
T Cell Response ◽  
T Cell Anergy ◽  
Cell Anergy

Abstract Staphylococcus aureus colonizes the skin of atopic dermatitis (AD) individuals, but the impact of its enterotoxins on the chronic activation of CD4+ T cells demands further analysis. We aimed to analyze the CD4+ T cell anergy profile and their phenotypic and functional features through differential expression of cellular activation markers, cytokine production and response to staphylococcal enterotoxin A (SEA). A panel of 84 genes relevant to T cell anergy was assessed by PCR array in FACS-sorted CD4+ T cells, and the most prominent genes were validated by RT-qPCR. We evaluated frequencies of circulating CD4+ T cells secreting single or multiple (polyfunctional) cytokines (IL-17A, IL-22, TNF, IFN-γ, and MIP-1β) and expression of activation marker CD38 in response to SEA stimulation by flow cytometry. Our main findings indicated upregulation of anergy-related genes (EGR2 and IL13) promoted by SEA in AD patients, associated to a compromised polyfunctional response particularly in CD4+CD38+ T cells in response to antigen stimulation. The pathogenic role of staphylococcal enterotoxins in adult AD can be explained by their ability to downmodulate the activated effector T cell response, altering gene expression profile such as EGR2 induction, and may contribute to negative regulation of polyfunctional CD4+ T cells in these patients.

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