Characterization of Murine Anti-Glycoprotein Ib Monoclonal Antibodies That Differentiate between Shear-Induced and Ristocetin/Botrocetin-Induced Glycoprotein Ib-von Willebrand Factor Interaction

2000 ◽  
Vol 30 (3) ◽  
pp. 139-148 ◽  
Author(s):  
N. Cauwenberghs ◽  
N. Ajzenberg ◽  
S. Vauterin ◽  
M.F. Hoylaerts ◽  
P.J. Declerck ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Von Willebrand Factor ◽  
Glycoprotein Ib ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Factor Interaction

scholarly journals Fibrinogen competes with von Willebrand factor for binding to the glycoprotein IIb/IIIa complex when platelets are stimulated with thrombin

Blood ◽  
1984 ◽  
Vol 64 (4) ◽  
pp. 797-800 ◽  
Author(s):  
HR Gralnick ◽  
SB Williams ◽  
BS Coller
Keyword(s):  
Monoclonal Antibodies ◽  
Von Willebrand Factor ◽  
Adenosine Diphosphate ◽  
Glycoprotein Ib ◽  
Human Fibrinogen ◽  
Factor Binding ◽  
Normal Plasma ◽  
Platelet Binding ◽  
Von Willebrand ◽  
Willebrand Factor

Two monoclonal antibodies--one that blocks ristocetin-induced platelet binding of von Willebrand factor to glycoprotein Ib and one that blocks adenosine diphosphate-induced binding of fibrinogen to the glycoprotein IIb/IIIa complex--were used to assess the binding site(s) for von Willebrand factor when platelets are stimulated with thrombin or adenosine diphosphate (ADP). Neither agonist induced binding of von Willebrand factor to glycoprotein Ib. ADP and thrombin induced von Willebrand factor binding exclusively to the glycoprotein IIb/IIIa complex. The results of the site of binding of von Willebrand factor with thrombasthenic platelets were consistent with the data obtained with the monoclonal antibodies and normal platelets. Human fibrinogen caused complete inhibition of thrombin-induced von Willebrand factor binding to normal platelets at concentrations considerably below that found in normal plasma. We conclude that thrombin induces very little binding of exogenous von Willebrand factor to platelets at normal plasma fibrinogen levels.

scholarly journals Production and characterization of monoclonal antibodies against von Willebrand factor (vWF).

1985 ◽  
Vol 16 (2) ◽  
pp. 205-207
Author(s):  
Midori SHIMA ◽  
Junji MORIMOTO ◽  
Shunsuke IMAI ◽  
Yoshihiko TSUBURA ◽  
Akira YOSHIOKA ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Von Willebrand Factor ◽  
Von Willebrand ◽  
Willebrand Factor

Characterization of a panel of monoclonal antibodies to von Willebrand Factor (vWF) and their use in a sensitive ELISA technique

1991 ◽  
Vol 61 ◽  
pp. 16
Author(s):  
M.L. Colibretti ◽  
A.B. Federici ◽  
S. Tombesi ◽  
R. Coppola ◽  
N. Di Rocco ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Von Willebrand Factor ◽  
Elisa Technique ◽  
Von Willebrand ◽  
Willebrand Factor
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