Improved Specificity of NRBC Detection in Chorionic Villus Sample Supernatant Fluids Using Anti-Zeta and Anti-Epsilon Monoclonal Antibodies

1999 ◽  
Vol 14 (5) ◽  
pp. 291-295 ◽  
Author(s):  
Ariadni Mavrou ◽  
Angeliki Kolialexi ◽  
Yun-Ling Zheng ◽  
Catherine Metaxotou ◽  
Diana W. Bianchi
Keyword(s):  
Monoclonal Antibodies ◽  
Chorionic Villus ◽  
Chorionic Villus Sample

DNA-based prenatal carrier detection for group a xeroderma pigmentosum in a chorionic villus sample

1995 ◽  
Vol 15 (7) ◽  
pp. 675-677 ◽  
Author(s):  
Naomichi Matsumoto ◽  
Nakamichi Saito ◽  
Naoki Harada ◽  
Kiyoji Tanaka ◽  
Norio Niikawa
Keyword(s):  
Xeroderma Pigmentosum ◽  
Chorionic Villus ◽  
Carrier Detection ◽  
Chorionic Villus Sample ◽  
Group A

Immunocytochemical identification of cytotrophoblast from other mononuclear cell populations isolated from first-trimester human chorionic villi

1985 ◽  
Vol 76 (1) ◽  
pp. 189-197
Author(s):  
B.H. Butterworth ◽  
Y.W. Loke
Keyword(s):  
Monoclonal Antibodies ◽  
Mononuclear Cell ◽  
Chorionic Villus ◽  
Cell Types ◽  
First Trimester ◽  
Cell Populations ◽  
Double Labelling ◽  
Chorionic Villi ◽  
Isolation Methods ◽  
Human Placental Cells

Trophoblast biologists are often uncertain as to what cell types they are investigating because the mononuclear cell populations prepared from trypsinization of human first-trimester chorionic villi are morphologically very similar. In the present study, immunocytochemical and phagocytic markers have been used to distinguish cytotrophoblast populations from cell types derived from the mesenchyme of the chorionic villus. Two anti-trophoblast monoclonal antibodies generated in our own laboratory (18B/A5 and 18A/C4) were found to be very efficient in identifying cytotrophoblast, which made up 35–40% of the cells in a smear. Most cytotrophoblast cells did not stain with a monoclonal anti-HLA-A,B,C antibody but a few cells (5%) were found to express both trophoblast and HLA-A,B,C antigens by a double-labelling technique. Endothelial cells from villous capillaries could be identified by a rabbit anti-factor VIII antibody. These cells formed 28% of the population in a cytospin smear. Macrophages from the villous mesenchyme were less readily separable as neither specific monoclonal antibodies nor localization of enzymes were found to be effective. However, these cells could be identified by their ability to phagocytose carmine. About 15% of the cells in a smear consisted of macrophages. The procedure described should prove useful in judging the efficiency of isolation methods from human placental cells.

Fetal RhD typing by DNA amplification in chorionic villus sample

The Lancet ◽  
1994 ◽  
Vol 344 (8927) ◽  
pp. 959-960 ◽  
Author(s):  
Bruno Brambati ◽  
Maria Chiara Anelli ◽  
Lucia Tului ◽  
Gabriele Colombo
Keyword(s):  
Chorionic Villus ◽  
Dna Amplification ◽  
Chorionic Villus Sample

Prenatal diagnosis of marfan syndrome: Identification of a fibrillin-1 mutation in chorionic villus sample

1995 ◽  
Vol 15 (12) ◽  
pp. 1176-1181 ◽  
Author(s):  
T. Rantamäki ◽  
M. Raghunath ◽  
L. Karttunen ◽  
L. Lönnqvist ◽  
A. Child ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Marfan Syndrome ◽  
Chorionic Villus ◽  
Chorionic Villus Sample ◽  
Fibrillin 1

scholarly journals Detection of Nucleated Erythrocytes (NRBC) In Chorionic Villus Sample Supernatant Fluid Using an anti-ε Monoclonal Antibody

1999 ◽  
Vol 45 (6) ◽  
pp. 924-924
Author(s):  
A Mavrou ◽  
A Kolialexi ◽  
E Kanavakis ◽  
Ch Vretou ◽  
C Metaxotou ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chorionic Villus ◽  
Supernatant Fluid ◽  
Chorionic Villus Sample

Ultrastructural analysis of unique glycoconjugates in the rat olfactory system

1992 ◽  
Vol 50 (1) ◽  
pp. 890-891
Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Olfactory System ◽  
Olfactory Bulbs ◽  
Temporal And Spatial Patterns ◽  
Carbohydrate Epitopes ◽  
Olfactory Systems ◽  
Temporal And Spatial ◽  
Cell Surface Glycoconjugates

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.

Structural and Chemical Studies of Pathological Fibers in Human Neurofibrillary Degeneration

1985 ◽  
Vol 43 ◽  
pp. 726-729
Author(s):  
K.S. Kosik ◽  
L.K. Duffy ◽  
S. Bakalis ◽  
C. Abraham ◽  
D.J. Selkoe
Keyword(s):  
Monoclonal Antibodies ◽  
Alzheimer Disease ◽  
Human Brain ◽  
Neurofibrillary Tangles ◽  
Morphological Analysis ◽  
High Specificity ◽  
Cytoskeletal Proteins ◽  
Neuritic Plaque ◽  
Protein Chemistry ◽  
Chemical Studies

The major structural lesions of the human brain during aging and in Alzheimer disease (AD) are the neurofibrillary tangles (NFT) and the senile (neuritic) plaque. Although these fibrous alterations have been recognized by light microscopists for almost a century, detailed biochemical and morphological analysis of the lesions has been undertaken only recently. Because the intraneuronal deposits in the NFT and the plaque neurites and the extraneuronal amyloid cores of the plaques have a filamentous ultrastructure, the neuronal cytoskeleton has played a prominent role in most pathogenetic hypotheses.The approach of our laboratory toward elucidating the origin of plaques and tangles in AD has been two-fold: the use of analytical protein chemistry to purify and then characterize the pathological fibers comprising the tangles and plaques, and the use of certain monoclonal antibodies to neuronal cytoskeletal proteins that, despite high specificity, cross-react with NFT and thus implicate epitopes of these proteins as constituents of the tangles.

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