Phenotypic Modifications of Human Mesangial Cells by Extracellular Matrix: The Importance of Matrix in the Contractile Response to Reactive Oxygen Species

2000 ◽  
Vol 8 (2) ◽  
pp. 97-103 ◽  
Author(s):  
M. Carmen Iglesias-de la Cruz ◽  
M. Piedad Ruiz-Torres ◽  
F. Javier De Lucio-Cazaña ◽  
Manuel Rodríguez-Puyol ◽  
Diego Rodríguez-Puyol
Keyword(s):  
Reactive Oxygen Species ◽  
Extracellular Matrix ◽  
Contractile Response ◽  
Mesangial Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Mesangial Cells

scholarly journals Effect of reactive oxygen species on endothelin-1 production by human mesangial cells

1996 ◽  
Vol 49 (1) ◽  
pp. 181-189 ◽  
Author(s):  
Alisa K. Hughes ◽  
Peter K. Stricklett ◽  
Eva Padilla ◽  
Donald E. Kohan
Keyword(s):  
Reactive Oxygen Species ◽  
Mesangial Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Mesangial Cells ◽  
Endothelin 1

scholarly journals Reactive oxygen species induce apoptosis in cultured human mesangial cells.

1996 ◽  
Vol 7 (11) ◽  
pp. 2357-2363
Author(s):  
H Sugiyama ◽  
N Kashihara ◽  
H Makino ◽  
Y Yamasaki ◽  
Z Ota
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Lactate Dehydrogenase ◽  
Xanthine Oxidase ◽  
Mesangial Cells ◽  
Reactive Oxygen ◽  
Radical Scavenger ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Human Mesangial Cells

Apoptosis is a distinct form of cell death that is observed under various physiologic and pathologic conditions, and it is thought to be important in regulating the number of glomerular cells. This study investigated the possible role of reactive oxygen species in the induction of apoptosis in cultured human mesangial cells. Fragmented nuclei with condensed chromatin, a morphologic characteristic of apoptosis, were observed by electron microscopy in mesangial cells exposed to 0.02 mM hydrogen peroxide for 4 h. Nuclear DNA extracted from mesangial cells that had been incubated with hydrogen peroxide (2 to 20 mM) or with xanthine (0.05 mM) and xanthine oxidase (5 to 100 mU/mL) showed the ladder pattern on electrophoresis that is a biochemical marker for apoptosis. Hydrogen peroxide (0.02 to 20 mM) decreased the number of viable cells, as determined by trypan blue exclusion, in a dose-dependent manner. Hydrogen peroxide or xanthine and xanthine oxidase increased the lactate dehydrogenase release from mesangial cells in a dose- and time-dependent manners. The release of lactate dehydrogenase was prevented by treatment with a free radical scavenger, catalase. Hydrogen peroxide (2 mM) also significantly increased the number of mesangial cells with fragmented DNA as detected by in situ nick end-labeling Results indicate that reactive oxygen species induce apoptosis in cultured human mesangial cells. Furthermore, apoptosis of mesangial cells induced by reactive oxygen species may contribute to the loss of such cells observed in glomerular disease.

scholarly journals Involvement of reactive oxygen species in proliferation of the cultured mouse mesangial cells

2000 ◽  
Vol 82 ◽  
pp. 249
Author(s):  
Hirofumi Yukumoto ◽  
Masakazu Osakada ◽  
Yoshiko Kawai ◽  
Munekazu Gemba
Keyword(s):  
Reactive Oxygen Species ◽  
Mesangial Cells ◽  
Reactive Oxygen ◽  
Oxygen Species
Sign in / Sign up

Export Citation Format

Share Document