Optimizing Ribozymes for Somatic Cell Gene Therapy

1998 ◽  
Vol 6 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Andrea D. Branch ◽  
Paul E. Klotman
Keyword(s):  
Gene Therapy ◽  
Somatic Cell ◽  
Somatic Cell Gene Therapy ◽  
Cell Gene

scholarly journals A murine model for B-lymphocyte somatic cell gene therapy.

1994 ◽  
Vol 91 (19) ◽  
pp. 8875-8879 ◽  
Author(s):  
N. Sutkowski ◽  
M. L. Kuo ◽  
A. Varela-Echavarria ◽  
J. P. Dougherty ◽  
Y. Ron
Keyword(s):  
Gene Therapy ◽  
Somatic Cell ◽  
Murine Model ◽  
B Lymphocyte ◽  
Somatic Cell Gene Therapy ◽  
Cell Gene

Transkaryotic implantation and the somatic cell gene therapy of diabetes

1990 ◽  
Vol 3 (1) ◽  
pp. 93
Author(s):  
Richard F. Selden ◽  
Mary Ellen Rowe ◽  
Howard M. Goodman
Keyword(s):  
Gene Therapy ◽  
Somatic Cell ◽  
Somatic Cell Gene Therapy ◽  
Cell Gene

Insulin delivery by somatic cell gene therapy

1993 ◽  
Vol 11 (3) ◽  
pp. 335-341 ◽  
Author(s):  
C Stewart ◽  
N A Taylor ◽  
K Docherty ◽  
C J Bailey
Keyword(s):  
Gene Therapy ◽  
Somatic Cell ◽  
Insulin Release ◽  
Human Insulin ◽  
Outer Region ◽  
Insulin Delivery ◽  
Control Group ◽  
C Peptide ◽  
Somatic Cell Gene Therapy ◽  
Cell Gene

ABSTRACT The feasibility of somatic cell gene therapy as a method of insulin delivery has been studied in mice. Murine pituitary AtT20 cells were transfected with a human preproinsulin DNA in a plasmid containing a metallothionein promoter and a gene conferring resistance to the antibiotic G418. The AtT20MtIns-1·4 clone of cells was selected because of its higher insulin-releasing activity compared with other clones. After culturing for 24 h in Dulbecco's medium containing 10 mM glucose, the AtT20MtIns-1·4 cells released human insulin at about 5 ng/106 cells per 24 h. Insulin release was not significantly altered by raised concentrations of glucose, potassium or calcium, but insulin release was increased by 20 mm arginine, 5 mm isomethylbutylxanthine and 90 μm zinc. AtT20MtIns-1·4 cells (2 × 106) were implanted intraperitoneally into non-diabetic athymic nude (nu/nu) mice, and the mice were made diabetic by injection of streptozotocin after 7 days. Release of human insulin in vivo was assessed using a specific plasma human C-peptide assay. Human C-peptide concentrations were maintained at about 01 pmol/ml throughout the 29 days of the study. The development of streptozotocin-induced hyperglycaemia was delayed in recipients of the cells releasing human insulin, compared with a control group receiving an implant of non-transfected cells. At autopsy the implanted AtT20MtIns-1·4 cells in each recipient had formed a tumour-like aggregation, with an outer region of insulin-containing cells. The study suggests that somatic cell gene therapy offers a feasible approach to insulin delivery.

Sign in / Sign up

Export Citation Format

Share Document