Swelling-Activated Chloride Currents in a Drug-Sensitive Cell Line and a P Glycoprotein-Expressing Derivative Are Underlied by Channels with the Same Pharmacological Properties

1998 ◽  
Vol 8 (5) ◽  
pp. 246-260 ◽  
Author(s):  
Julie K. Horton ◽  
Carlos G. Vanoye ◽  
Luis Reuss
Keyword(s):  
Cell Line ◽  
Pharmacological Properties ◽  
Sensitive Cell ◽  
Sensitive Cell Line ◽  
Chloride Currents ◽  
P Glycoprotein

scholarly journals Phytochemical Analysis and In Vitro Effects of Allium fistulosum L. and Allium sativum L. Extracts on Human Normal and Tumor Cell Lines: A Comparative Study

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 574
Author(s):  
Adrian Bogdan Țigu ◽  
Cristian Silviu Moldovan ◽  
Vlad-Alexandru Toma ◽  
Anca Daniela Farcaș ◽  
Augustin Cătălin Moț ◽  
...  
Keyword(s):  
Cell Line ◽  
Allium Sativum ◽  
Allium Fistulosum ◽  
Tumor Cell Lines ◽  
Sensitive Cell ◽  
Sensitive Cell Line ◽  
Welsh Onion ◽  
Normal Cells ◽  
Ic50 Value ◽  
High Concentrations

Allium sativum L. (garlic bulbs) and Allium fistulosum L. (Welsh onion leaves) showed quantitative differences of identified compounds: allicin and alliin (380 µg/mL and 1410 µg/mL in garlic; 20 µg/mL and 145 µg/mL in Welsh onion), and the phenolic compounds (chlorogenic acid, p-coumaric acid, ferulic acid, gentisic acid, 4-hydroxybenzoic acid, kaempferol, isoquercitrin, quercitrin, quercetin, and rutin). The chemical composition determined the inhibitory activity of Allium extracts in a dose-dependent manner, on human normal cells (BJ-IC50 0.8841% garlic/0.2433% Welsh onion and HaCaT-IC50 1.086% garlic/0.6197% Welsh onion) and tumor cells (DLD-1-IC50 5.482%/2.124%; MDA-MB-231-IC50 6.375%/2.464%; MCF-7-IC50 6.131%/3.353%; and SK-MES-1-IC50 4.651%/5.819%). At high concentrations, the cytotoxic activity of each extract, on normal cells, was confirmed by: the 50% of the growth inhibition concentration (IC50) value, the cell death induced by necrosis, and biochemical determination of LDH, catalase, and Caspase-3. The four tumor cell lines treated with high concentrations (10%, 5%, 2.5%, and 1.25%) of garlic extract showed different sensibility, appreciated on the base of IC50 value for the most sensitive cell line (SK-MES-1), and the less sensitive (MDA-MB-231) cell line. The high concentrations of Welsh onion extract (5%, 2.5%, and 1.25%) induced pH changes in the culture medium and SK-MES-1 being the less sensitive cell line.

scholarly journals Functional IsK/KvLQT1 Potassium Channel in a New Corticosteroid-sensitive Cell Line Derived from the Inner Ear

2006 ◽  
Vol 281 (15) ◽  
pp. 10496-10507 ◽  
Author(s):  
Marie Teixeira ◽  
Say Viengchareun ◽  
Daniel Butlen ◽  
Chrystophe Ferreira ◽  
Françoise Cluzeaud ◽  
...  
Keyword(s):  
Potassium Channel ◽  
Inner Ear ◽  
Cell Line ◽  
Sensitive Cell ◽  
Sensitive Cell Line

NO is not sufficient to explain maximal cytotoxicity of tumoricidal macrophages against an NO-sensitive cell line

1996 ◽  
Vol 60 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Romeo B. Mateo ◽  
Jonathan S. Reichner ◽  
Jorge E. Albina
Keyword(s):  
Cell Line ◽  
Sensitive Cell ◽  
Sensitive Cell Line

Difference of Drug-Resistance between Single-Factor Resistance Cell Line K562/MDR1 Transfected with mdr1 Gene and Multi-Factor Resistance Cell Line K562/A02 Induced by Doxorubicin.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4179-4179
Author(s):  
Yuping Gong ◽  
Xueshi Ye ◽  
Ting Liu
Keyword(s):  
Drug Resistance ◽  
Cell Line ◽  
Cell Lines ◽  
Anticancer Drug ◽  
Leukemia Cell Line ◽  
Mdr1 Gene ◽  
Sensitive Cell ◽  
Single Factor ◽  
Efflux Rate ◽  
Sensitive Cell Line

Abstract Objective To study difference of drug-resistance between single-factor resistance cell line K562/MDR1 transfected with mdr1 gene and multi-factor resistance cell line K562/A02 induced by doxorubicin. Methods Retroviral virions carrying the complete sequence of mdr1 gene cDNA were produced and infected drug-sensitive leukemia cell line K562 and mdr1 single-factor resistance cell line K562/MDR1 was established. The difference of drug-resistance between K562/MDR1 and K562/A02, a kind of multi-factor resistance cell line induced by doxorubicin, was studied by checking the expression of mdr1 gene and Pgp, daunorubicin efflux rate, MTT drug sensitivity to chemotherapeutic drug. Lentiviral vector encoding shRNA which targeted MDR1 gene was transfected into two kinds of cell lines and effect of RNAi on reversing drug resistance was detected. Results The results of Q-PCR and flow cytometry demonstrated that there were high expression of mdr1 mRNA and Pgp in both kinds of drug-resistance cell lines and no difference between them. The function of Pgp detected by daunorubicin efflux rate is higher in K562/MDR1 (90.93%) than K562/A02 (78.67%). The results of MTT test showed that IC50 of K562/MDR1 and K562/A02 is 0.55 and 1.22μmol/L respectively and this confirmed that drug-resistance in K562/A02 is higher than that in K562/MDR1. After RNA interference, the expression of the mdr1 gene and Pgp in K562/MDR1 markedly was down-regulated and the drug resistance was restored and IC50 is 0.16μmol/L, similar to K562 sensitive cell line. The expression of the mdr1 gene and Pgp in K562/A02 markedly was downregulated too, and drug resistance to anticancer drug is reduced to some extent but IC50 is 0.56μmol/L, it is still higher than that in sensitive cell line. Conclusion Drug-resistance in K562/A02 induced by anticancer-drug was made of many factors and it is more resistance to anticancer-drug than that in K562/MDR1 caused by mdr1 gene. Due to only mdr1 resistance, K562/MDR1 is better cell model to make mdr1/Pgp research.

Chromosomal alterations associated with transformation of a tamoxifen-sensitive cell line into its drug resistant clone

2001 ◽  
Vol 37 ◽  
pp. 29-30
Author(s):  
R. Achuthan ◽  
S.M. Bell ◽  
P. Roberts ◽  
K. Horgan ◽  
A.F. Markham ◽  
...  
Keyword(s):  
Cell Line ◽  
Drug Resistant ◽  
Sensitive Cell ◽  
Resistant Clone ◽  
Sensitive Cell Line ◽  
Chromosomal Alterations
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