Chromosomal localization1 of the human cytoplasmic dynein heavy chain gene DNCH2 to 11q21→q22.1

1998 ◽  
Vol 82 (1-2) ◽  
pp. 123-125
Author(s):  
M.R. Koehler ◽  
M. Schmid ◽  
J. Neesen
Keyword(s):  
Heavy Chain ◽  
Chromosomal Localization ◽  
Cytoplasmic Dynein ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Dynein Heavy Chain

Cytoplasmic Dynein Function Is Essential in Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 865-878 ◽  
Author(s):  
Janice Gepner ◽  
Min-gang Li ◽  
Susan Ludmann ◽  
Cynthia Kortas ◽  
Kristin Boylan ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Intracellular Transport ◽  
Mutational Analysis ◽  
Cytoplasmic Dynein ◽  
Complementation Group ◽  
Transport Processes ◽  
Female Sterility ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Dynein Heavy Chain

Abstract The microtubule motor cytoplasmic dynein has been implicated in a variety of intracellular transport processes. We previously identified and characterized the Drosophila gene Dhc64C, which encodes a cytoplasmic dynein heavy chain. To investigate the function of the cytoplasmic dynein motor, we initiated a mutational analysis of the Dhc64C dynein gene. A small deletion that removes the chromosomal region containing the heavy chain gene was used to isolate EMS-induced lethal mutations that define at least eight essential genes in the region. Germline transformation with a Dhc64C transgene rescued 16 mutant alleles in the single complementation group that identifies the dynein heavy chain gene. All 16 alleles were hemizygous lethal, which demonstrates that the cytoplasmic dynein heavy chain gene Dhc64C is essential for Drosophila development. Furthermore, our failure to recover somatic clones of cells homozygous for a Dhc64C mutation indicates that cytoplasmic dynein function is required for cell viability in several Drosophila tissues. The intragenic complementation of dynein alleles reveals multiple mutant phenotypes including male and/or female sterility, bristle defects, and defects in eye development.

Identification and molecular evolution of new dynein-like protein sequences in rat brain

1995 ◽  
Vol 108 (5) ◽  
pp. 1883-1893 ◽  
Author(s):  
Y. Tanaka ◽  
Z. Zhang ◽  
N. Hirokawa
Keyword(s):  
Rat Brain ◽  
Heavy Chain ◽  
Phylogenetic Trees ◽  
Cytoplasmic Dynein ◽  
Amino Acid Sequences ◽  
Adult Brain ◽  
Chain Gene ◽  
Evolutionary Analysis ◽  
Degenerate Primers ◽  
Dynein Heavy Chain

RT-PCR cloning was performed to find unknown members of the dynein superfamily expressed in rat brain. Six kinds of degenerate primers designed for the dynein catalytic domain consensuses were used for extensive PCR amplifications. We have sequenced 550 plasmid clones which turned out to include 13 kinds of new dynein-like sequences (DLP1-8, 9A/B, 10–12) and cytoplasmic dynein heavy chain. In these clones, alternative splicing was detected for a 105 nt-domain containing the CFDEFNRI consensus just downstream of the most N-terminal P-loop (DLP9A and 9B). By using these obtained sequences, initial hybridization studies were performed. Genomic Southern blotting showed each sequence corresponds to a single copy of the gene, while northern blotting of adult brain presented more than one band for some subtypes. We further accomplished molecular evolutionary analysis to recognize their phylogenetic origins for the axonemal and non-axonemal (cytoplasmic) functions. Different methods (UPGMA, NJ and MP) presented well coincident phylogenetic trees from 44 partial amino acid sequences of dynein heavy chain from various eukaryotes. The trunk for all the cytoplasmic dynein heavy chain homologues diverged directly from the root of the phylogenetic tree, suggesting that the first dynein gene duplication defined two distinct functions as respective subfamilies. Of particular interest, we found a duplication event of the cytoplasmic dynein heavy chain gene giving rise to another subtype, DLP4, located between the divergence of yeast and that of Dictyostelium. Such evolutionary topology builds up an inceptive hypothesis that there are at least two non-axonemal dynein heavy chains in mammals.

Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena

2003 ◽  
pp. 017-027
Author(s):  
Gangadhara Sailaja ◽  
Leslie M. Lincoln ◽  
Jifan Chen ◽  
David J. Asai
Keyword(s):  
Gene Family ◽  
Heavy Chain ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Dynein Heavy Chain

scholarly journals A mutation in the dynein heavy chain gene compensates for energy deficit of mutant SOD1 mice and increases potentially neuroprotective IGF-1

2011 ◽  
Vol 6 (1) ◽  
pp. 26 ◽  
Author(s):  
Anissa Fergani ◽  
Judith Eschbach ◽  
Hugues Oudart ◽  
Yves Larmet ◽  
Birgit Schwalenstocker ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Energy Deficit ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Mutant Sod1 ◽  
Dynein Heavy Chain
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